Biphasic vesicle: instability induced by adsorptionof protein s

The recent discovery of a lateral organization in cell membranes due to small structures called ‘rafts’ has motivated a lot of biological and physico-chemical studies. A new experiment on a model system has showna spectacular buddin g process with the expulsionof on e or two rafts when one introduces proteins on the membrane. In this paper, we give a physical interpretation of the budding of the raft phase. An approach based on the energy of the system including the presence of proteins is used to derive a shape equation and to study possible instabilities. This model shows two di4erent situations which are strongly dependent on the nature of the proteins: a regime of easy budding when the proteins are strongly coupled to the membrane and a regime of di5cult budding. c

[1]  S. Singer,et al.  The Fluid Mosaic Model of the Structure of Cell Membranes , 1972, Science.

[2]  Deborah A. Brown,et al.  Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface , 1992, Cell.

[3]  G. Temple.  Static and Dynamic Electricity , 1940, Nature.

[4]  Sarah L Veatch,et al.  Organization in lipid membranes containing cholesterol. , 2002, Physical review letters.

[5]  P. Gennes,et al.  Transient pores in stretched vesicles: role of leak-out , 2000, Physica A: Statistical Mechanics and its Applications.

[6]  Seifert,et al.  Shape transformations of vesicles: Phase diagram for spontaneous- curvature and bilayer-coupling models. , 1991, Physical review. A, Atomic, molecular, and optical physics.

[7]  Sphingolipid Metabolism and Signaling Minireview Series* , 2002, The Journal of Biological Chemistry.

[8]  Tian-yun Wang,et al.  Sphingolipid partitioning into ordered domains in cholesterol-free and cholesterol-containing lipid bilayers. , 2003, Biophysical journal.

[9]  Seifert,et al.  Curvature-induced lateral phase segregation in two-component vesicles. , 1993, Physical review letters.

[10]  C. Marques,et al.  Pressure patches for membranes: the induced pinch of a grafted polymer. , 2000, Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics.

[11]  Seifert,et al.  Vesicular instabilities: The prolate-to-oblate transition and other shape instabilities of fluid bilayer membranes. , 1995, Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics.

[12]  Akihiro Kusumi,et al.  Relationship of lipid rafts to transient confinement zones detected by single particle tracking. , 2002, Biophysical journal.

[13]  A. Love.  A treatise on the mathematical theory of elasticity , 1892 .

[14]  P. Canham.  The minimum energy of bending as a possible explanation of the biconcave shape of the human red blood cell. , 1970, Journal of theoretical biology.

[15]  Watt W. Webb,et al.  Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension , 2003, Nature.

[16]  U. Seifert,et al.  Mapping vesicle shapes into the phase diagram: A comparison of experiment and theory , 1996, cond-mat/9612151.

[17]  Udo Seifert,et al.  Configurations of fluid membranes and vesicles , 1997 .

[18]  G. Meer,et al.  The Different Hues of Lipid Rafts , 2002 .

[19]  G. V. Haines.  Spherical cap harmonic analysis , 1985 .

[20]  G. van Meer,et al.  Sphingolipid Transport: Rafts and Translocators* , 2002, The Journal of Biological Chemistry.

[21]  Nicolas Destainville,et al.  Confined diffusion without fences of a g-protein-coupled receptor as revealed by single particle tracking. , 2003, Biophysical journal.

[22]  R. Lipowsky,et al.  Shape transformations of vesicles with intramembrane domains. , 1996, Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics.

[23]  A Ajdari,et al.  Effective-area elasticity and tension of micromanipulated membranes. , 2001, Physical review letters.

[24]  P. Higgs,et al.  Theory of fission for two-component lipid vesicles , 1997 .

[25]  E Gratton,et al.  Lipid rafts reconstituted in model membranes. , 2001, Biophysical journal.

[26]  E. Ikonen,et al.  Functional rafts in cell membranes , 1997, Nature.

[27]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[28]  Deborah A. Brown,et al.  Structure and Function of Sphingolipid- and Cholesterol-rich Membrane Rafts* , 2000, The Journal of Biological Chemistry.

[29]  Rao,et al.  Equilibrium budding and vesiculation in the curvature model of fluid lipid vesicles. , 1991, Physical review. A, Atomic, molecular, and optical physics.

[30]  Curvature Instability Induced by Diffusing Particles , 1993 .

[31]  S. Leibler,et al.  Curvature instability in membranes , 1986 .