Development of biomimetic thermoplastic polyurethane/fibroin small-diameter vascular grafts via a novel electrospinning approach.

A new electrospinning approach for fabricating vascular grafts with a layered, circumferentially aligned, and micro-wavy fibrous structure similar to natural elastic tissues has been developed. The customized electrospinning collector was able to generate wavy fibers using the dynamic "jump rope" collecting process, which also solved the sample removal problem for mandrel-type collectors. In this study, natural silk fibroin and synthetic thermoplastic polyurethane (TPU) were combined at different weight ratios to produce hybrid small-diameter vascular grafts. The purpose of combining these two materials was to leverage the bioactivity and tunable mechanical properties of these natural and synthetic materials. Results showed that the electrospun fiber morphology was highly influenced by the material compositions and solvents employed. All of the TPU/fibroin hybrid grafts had mechanical properties comparable to natural blood vessels. The circumferentially aligned and wavy biomimetic configuration provided the grafts with a sufficient toe region and the capacity for long-term usage under repeated dilatation and contraction. Cell culture tests with human endothelial cells (EC) also revealed high cell viability and good biocompatibility for these grafts. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 985-996, 2018.

[1]  Lih-Sheng Turng,et al.  Biocompatible, degradable thermoplastic polyurethane based on polycaprolactone-block-polytetrahydrofuran-block-polycaprolactone copolymers for soft tissue engineering. , 2017, Journal of materials chemistry. B.

[2]  S. Margulies,et al.  Repeated Loading Behavior of Pediatric Porcine Common Carotid Arteries. , 2016, Journal of biomechanical engineering.

[3]  J. Thomson,et al.  Fabrication and Characterization of Electrospun Thermoplastic Polyurethane/Fibroin Small-Diameter Vascular Grafts for Vascular Tissue Engineering , 2016, International polymer processing : the journal of the Polymer Processing Society.

[4]  Lih-Sheng Turng,et al.  Approaches to Fabricating Multiple-Layered Vascular Scaffolds Using Hybrid Electrospinning and Thermally Induced Phase Separation Methods , 2016 .

[5]  Xiangfang Peng,et al.  Fabrication of triple-layered vascular scaffolds by combining electrospinning, braiding, and thermally induced phase separation , 2015 .

[6]  Sheila MacNeil,et al.  The Tissue-Engineered Vascular Graft—Past, Present, and Future , 2015, Tissue engineering. Part B, Reviews.

[7]  Xiangfang Peng,et al.  Properties and fibroblast cellular response of soft and hard thermoplastic polyurethane electrospun nanofibrous scaffolds. , 2015, Journal of biomedical materials research. Part B, Applied biomaterials.

[8]  Charanpreet Singh,et al.  Medical Textiles as Vascular Implants and Their Success to Mimic Natural Arteries , 2015, Journal of functional biomaterials.

[9]  Xiangfang Peng,et al.  Electrospinning thermoplastic polyurethane/graphene oxide scaffolds for small diameter vascular graft applications. , 2015, Materials science & engineering. C, Materials for biological applications.

[10]  L. Turng,et al.  Fabrication of porous synthetic polymer scaffolds for tissue engineering , 2015 .

[11]  Lih-Sheng Turng,et al.  Electrospinning of unidirectionally and orthogonally aligned thermoplastic polyurethane nanofibers: fiber orientation and cell migration. , 2015, Journal of biomedical materials research. Part A.

[12]  Silvia Farè,et al.  Vascular Tissue Engineering: Recent Advances in Small Diameter Blood Vessel Regeneration , 2014 .

[13]  Yong Huang,et al.  Electrospun tubular scaffold with circumferentially aligned nanofibers for regulating smooth muscle cell growth. , 2014, ACS applied materials & interfaces.

[14]  Marissa Nichole Rylander,et al.  The influence of electrospun scaffold topography on endothelial cell morphology, alignment, and adhesion in response to fluid flow , 2014, Biotechnology and bioengineering.

[15]  Diego Mantovani,et al.  Small-diameter vascular tissue engineering , 2013, Nature Reviews Cardiology.

[16]  V. Pillay,et al.  A Review of the Effect of Processing Variables on the Fabrication of Electrospun Nanofibers for Drug Delivery Applications , 2013 .

[17]  Wenjie Yuan,et al.  Co-electrospun blends of PU and PEG as potential biocompatible scaffolds for small-diameter vascular tissue engineering , 2012 .

[18]  M. Navidbakhsh,et al.  Comparison between mechanical properties of human saphenous vein and umbilical vein , 2012, Biomedical engineering online.

[19]  N. Miki,et al.  Solution parameters for the fabrication of thinner silicone fibers by electrospinning. , 2012, Physical review. E, Statistical, nonlinear, and soft matter physics.

[20]  Robert J Levy,et al.  Micropatterning of three-dimensional electrospun polyurethane vascular grafts. , 2010, Acta biomaterialia.

[21]  Jintu Fan,et al.  Electrospinning of small diameter 3-D nanofibrous tubular scaffolds with controllable nanofiber orientations for vascular grafts , 2010, Journal of materials science. Materials in medicine.

[22]  Diego Mantovani,et al.  Compliant electrospun silk fibroin tubes for small vessel bypass grafting. , 2010, Acta biomaterialia.

[23]  David G Simpson,et al.  A three-layered electrospun matrix to mimic native arterial architecture using polycaprolactone, elastin, and collagen: a preliminary study. , 2010, Acta biomaterialia.

[24]  S. Sano,et al.  Suture retention strength of expanded polytetrafluoroethylene (ePTFE) graft. , 2010, Acta medica Okayama.

[25]  M. Maaza,et al.  The influence of electrospinning parameters on the structural morphology and diameter of electrospun nanofibers , 2010 .

[26]  G. Bowlin,et al.  Electrospinning-aligned and random polydioxanone–polycaprolactone–silk fibroin-blended scaffolds: geometry for a vascular matrix , 2009, Biomedical materials.

[27]  Heinrich Schima,et al.  Electrospun polyurethane vascular grafts: in vitro mechanical behavior and endothelial adhesion molecule expression. , 2009, Journal of biomedical materials research. Part A.

[28]  Wei He,et al.  Tubular nanofiber scaffolds for tissue engineered small-diameter vascular grafts. , 2009, Journal of biomedical materials research. Part A.

[29]  Darrell H. Reneker,et al.  Electrospinning jets and polymer nanofibers , 2008 .

[30]  Matthew P. Brennan,et al.  Small-diameter biodegradable scaffolds for functional vascular tissue engineering in the mouse model. , 2008, Biomaterials.

[31]  Jan P Stegemann,et al.  Review: advances in vascular tissue engineering using protein-based biomaterials. , 2007, Tissue engineering.

[32]  Michael J Sherratt,et al.  Applying elastic fibre biology in vascular tissue engineering , 2007, Philosophical Transactions of the Royal Society B: Biological Sciences.

[33]  J. Lannutti,et al.  Electrospinning for tissue engineering scaffolds , 2007 .

[34]  Tzu-Wei Wang,et al.  Coculture of endothelial and smooth muscle cells on a collagen membrane in the development of a small-diameter vascular graft. , 2007, Biomaterials.

[35]  A. Mikos,et al.  Electrospinning of polymeric nanofibers for tissue engineering applications: a review. , 2006, Tissue engineering.

[36]  R. Black,et al.  PCL-PU composite vascular scaffold production for vascular tissue engineering: attachment, proliferation and bioactivity of human vascular endothelial cells. , 2006, Biomaterials.

[37]  Seung Jin Lee,et al.  Effect of solution properties on nanofibrous structure of electrospun poly(lactic‐co‐glycolic acid) , 2006 .

[38]  Gerhard Sommer,et al.  Determination of layer-specific mechanical properties of human coronary arteries with nonatherosclerotic intimal thickening and related constitutive modeling. , 2005, American journal of physiology. Heart and circulatory physiology.

[39]  F P T Baaijens,et al.  Design of scaffolds for blood vessel tissue engineering using a multi-layering electrospinning technique. , 2005, Acta biomaterialia.

[40]  Alexander M Seifalian,et al.  Current status of prosthetic bypass grafts: a review. , 2005, Journal of biomedical materials research. Part B, Applied biomaterials.

[41]  S. Andreadis,et al.  Engineering of fibrin-based functional and implantable small-diameter blood vessels. , 2005, American journal of physiology. Heart and circulatory physiology.

[42]  Debby Gawlitta,et al.  Properties of engineered vascular constructs made from collagen, fibrin, and collagen-fibrin mixtures. , 2004, Biomaterials.

[43]  Peter X. Ma,et al.  Scaffolds for tissue fabrication , 2004 .

[44]  M. Kotaki,et al.  A review on polymer nanofibers by electrospinning and their applications in nanocomposites , 2003 .

[45]  Cornelius Borst,et al.  Mechanical properties of porcine and human arteries: implications for coronary anastomotic connectors. , 2003, The Annals of thoracic surgery.

[46]  Bartley P Griffith,et al.  Effect of aneurysm on the tensile strength and biomechanical behavior of the ascending thoracic aorta. , 2003, The Annals of thoracic surgery.

[47]  Athanassios Sambanis,et al.  A biological hybrid model for collagen-based tissue engineered vascular constructs. , 2003, Biomaterials.

[48]  A. Seifalian,et al.  New Prostheses for Use in Bypass Grafts with Special Emphasis on Polyurethanes , 2002, Cardiovascular surgery.

[49]  Robert M. Nerem,et al.  Dynamic Mechanical Conditioning of Collagen-Gel Blood Vessel Constructs Induces Remodeling In Vitro , 2000, Annals of Biomedical Engineering.

[50]  Darrell H. Reneker,et al.  Electrospinning process and applications of electrospun fibers , 1993, Conference Record of the 1993 IEEE Industry Applications Conference Twenty-Eighth IAS Annual Meeting.

[51]  Ali Khademhosseini,et al.  Electrospun scaffolds for tissue engineering of vascular grafts. , 2014, Acta biomaterialia.

[52]  R. Gurny,et al.  Long term performance of polycaprolactone vascular grafts in a rat abdominal aorta replacement model. , 2012, Biomaterials.

[53]  Steven G Wise,et al.  A multilayered synthetic human elastin/polycaprolactone hybrid vascular graft with tailored mechanical properties. , 2011, Acta biomaterialia.

[54]  Dimitrios P Sokolis,et al.  Ascending thoracic aortic aneurysms are associated with compositional remodeling and vessel stiffening but not weakening in age-matched subjects. , 2009, The Journal of thoracic and cardiovascular surgery.

[55]  R Langer,et al.  Stabilized polyglycolic acid fibre-based tubes for tissue engineering. , 1996, Biomaterials.