Rapid methods for deoxynivalenol and other trichothecenes.

Method development for deoxynivalenol (DON) and other trichothecenes in recent years was driven by the analytical necessities arising from its widespread (and increasing) occurrence in foods and feeds. This has resulted in the establishment of guideline levels for animal feed, tolerable daily intake (TDI) levels for humans, and most importantly, in the prospect of low-tolerance levels for these toxins in foods in the near future. In order to ensure reliable determination of the toxin content at the tolerance levels, routine analytical methods must have detection limits of less than the tolerance level. This paper intends to give an overview of current analytical developments of rapid testing for deoxynivalenol and other trichothecene mycotoxins, with a special focus on antibody-based techniques. This includes high-throughput instrumental analysis for the laboratory environment, as well as rapid visual tests for on-site testing. The applicability of rapid tests within an integrated detection system for mycotoxins in foods is discussed.

[1]  C. V. Van Peteghem,et al.  Development of a portable field immunoassay for the detection of aflatoxin M1 in milk. , 1999, International journal of food microbiology.

[2]  Liberty Sibanda,et al.  A collaborative study to validate novel field immunoassay kits for rapid mycotoxin detection. , 2002, International journal of food microbiology.

[3]  F. Chu,et al.  Production and Characterization of Antibody Against Deoxynivalenol Triacetate. , 1986, Journal of food protection.

[4]  C. Maragos,et al.  Fluorescence polarization as a means for determination of fumonisins in maize. , 2001, Journal of agricultural and food chemistry.

[5]  E. Märtlbauer,et al.  Two formats of enzyme immunoassay for 15-acetyldeoxynivalenol applied to wheat , 1993 .

[6]  R. Dietrich,et al.  Direct enzyme-linked immunosorbent assays for the detection of the 8-ketotrichothecene mycotoxins deoxynivalenol, 3-acetyldeoxynivalenol, and 15-acetyldeoxynivalenol in buffer solutions , 1991 .

[7]  E. Märtlbauer,et al.  Production and characterization of monoclonal antibodies to the macrocyclic trichothecene roridin A , 1988, Applied and environmental microbiology.

[8]  E. Märtlbauer,et al.  Preparation and characterization of polyclonal and monoclonal antibodies against fusarenon‐X , 1989 .

[9]  J. M. Fremy,et al.  Policy on characterization of antibodies used in immunochemical methods of analysis for mycotoxins and phycotoxins. , 2003, Journal of AOAC International.

[10]  S. Kruger,et al.  Quantification of deoxynivalenol in wheat using an immunoaffinity column and liquid chromatography. , 1999, Journal of chromatography. A.

[11]  Danfeng Yao,et al.  Surface plasmon field-enhanced fluorescence spectroscopy studies of the interaction between an antibody and its surface-coupled antigen. , 2003, Analytical chemistry.

[12]  E. Märtlbauer,et al.  Detection of acetylated deoxynivalenol by enzyme-linked immunosorbent assay. , 1996 .

[13]  R. Dietrich,et al.  Use of monoclonal antibodies for the analysis of mycotoxins. , 1995, Natural toxins.

[14]  C. V. Van Peteghem,et al.  Detection of T-2 toxin in different cereals by flow-through enzyme immunoassay with a simultaneous internal reference. , 2000, Journal of agricultural and food chemistry.

[15]  G. Christian,et al.  Flow injection renewable surface immunoassay for real time monitoring of biospecific interactions. , 1997, Analytical chemistry.

[16]  F. Chu,et al.  Enzyme-linked immunosorbent assay for deoxynivalenol in corn and wheat. , 1988, Journal - Association of Official Analytical Chemists.

[17]  J. Pestka,et al.  Enzyme-linked immunosorbent assay employing monoclonal antibody specific for deoxynivalenol (vomitoxin) and several analogues , 1988 .

[18]  C. Maragos,et al.  Fluorescence polarization as a tool for the determination of deoxynivalenol in wheat , 2002, Food additives and contaminants.

[19]  C. Miles,et al.  Production of a monoclonal antibody with specificity for deoxynivalenol, 3‐acetyldeoxynivalenol and 15‐acetyldeoxynivalenol , 1993 .

[20]  L. Stanker,et al.  Recent studies on immunoassays for mycotoxins. , 1996 .

[21]  R. Dietrich,et al.  Development and application of immunochromatographic tests for the detection of staphylococcal enterotoxin E , 1998 .

[22]  J Gilbert,et al.  Immunoaffinity column cleanup with liquid chromatography for determination of aflatoxin M1 in liquid milk: collaborative study. , 2001, Journal of AOAC International.

[23]  E. Anklam,et al.  Determination of aflatoxin B1 in baby food (infant formula) by immunoaffinity column cleanup liquid chromatography with postcolumn bromination: collaborative study. , 2001, Journal of AOAC International.

[24]  E. Lai,et al.  Immunoassay of fumonisins by a surface plasmon resonance biosensor. , 1998, Analytical biochemistry.

[25]  R. Dietrich,et al.  Detection of aflatoxins, trichothecenes, ochratoxin a and zearalenone by test strip enzyme immunoassay: A rapid method for screening cereals for mycotoxins , 1991 .

[26]  C. Maragos,et al.  Monoclonal Antibodies for the Mycotoxins Deoxynivalenol and 3-Acetyl-Deoxynivalenol , 2000 .

[27]  L. Stanker,et al.  Immunoassays for residue analysis: food safety , 1996 .

[28]  Ewald Usleber,et al.  Rapid Detection of Fumonisin B1 in Corn-Based Food by Competitive Direct Dipstick Enzyme Immunoassay/Enzyme-Linked Immunofiltration Assay with Integrated Negative Control Reaction , 1995 .

[29]  E. Märtlbauer,et al.  A monoclonal antibody‐based enzyme immunoassay for the detection of T‐2 toxin at picogram levels , 1989 .

[30]  E. Stigter,et al.  Rapid surface plasmon resonance-based inhibition assay of deoxynivalenol. , 2003, Journal of agricultural and food chemistry.

[31]  R. Vogel,et al.  A Biosensor-based Immunoassay for Rapid Screening of Deoxynivalenol Contamination in Wheat , 2002 .

[32]  D. Undersander,et al.  Immunoassays of Selected Mycotoxins in Hay, Silage and Mixed Feed , 1999 .

[33]  R. Krska,et al.  An enzyme linked immunoassay for the determination of deoxynivalenol in wheat based on chicken egg yolk antibodies , 2000, Fresenius' journal of analytical chemistry.

[34]  E. Mills,et al.  An enzyme‐linked immunosorbent assay for deoxynivalenol in wheat, utilizing novel hapten derivatization procedures , 1990 .

[35]  M. Savard,et al.  Production of monoclonal antibodies for the specific detection of deoxynivalenol and 15-acetyldeoxynivalenol by ELISA , 1995 .

[36]  R. Dietrich,et al.  Multimycotoxin dipstick enzyme immunoassay applied to wheat. , 1995, Food additives and contaminants.

[37]  M. Morgan,et al.  Development of surface plasmon resonance-based immunoassay for aflatoxin B(1). , 2000, Journal of agricultural and food chemistry.