Electrochemotherapy potentiation of antitumour effect of bleomycin by local electric pulses.

In cell culture the cytotoxicity of some anticancer drugs, especially bleomycin, can be greatly enhanced by exposing cells to non-cytotoxic electric pulses. Nude or conventional mice bearing subcutaneous transplanted tumours were treated with intramuscular doses of bleomycin followed by local delivery of electric pulses similar to those used in vitro. Tumors were reduced and even eradicated after this electrochemotherapy. Thus the antitumour effects of bleomycin in mice can be considerably potentiated by local electric pulses.

[1]  Bradner Wt,et al.  Bleomycin, a review. , 1976 .

[2]  D. Fradelizi,et al.  An improved electrotransfection method using square shaped electric impulsions. , 1988, Biochemical and biophysical research communications.

[3]  S. Horwitz,et al.  Characterization of the association of radiolabeled bleomycin A2 with HeLa cells. , 1984, Cancer research.

[4]  J. Belehradek,et al.  Evolution of cell‐mediated antitumor immunity in mice bearing a syngeneic chemically induced tumor. Influence of tumor growth, surgical removal and treatment with irradiated tumor cells , 1972, International journal of cancer.

[5]  L. Mir,et al.  Introduction of definite amounts of nonpermeant molecules into living cells after electropermeabilization: direct access to the cytosol. , 1988, Experimental cell research.

[6]  J. Teissié,et al.  Electric field mediated transformation: isolation and characterization of a TK+ subclone. , 1985, Biochemical and biophysical research communications.

[7]  H. Eagle,et al.  Propagation in a Fluid Medium of a Human Epidermoid Carcinoma, Strain KB , 1955, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[8]  E. Neumann,et al.  Gene transfer into mouse lyoma cells by electroporation in high electric fields. , 1982, The EMBO journal.

[9]  L. Mir,et al.  Voltage-dependent introduction of a d[α]octothymidylate into electropermeabilized cells , 1989 .

[10]  L. Mir,et al.  Transient electropermeabilization of cells in culture. Increase of the cytotoxicity of anticancer drugs. , 1988, Biochemical pharmacology.

[11]  R. Auerbach,et al.  Regional differences in the incidence and growth of mouse tumors following intradermal or subcutaneous inoculation. , 1978, Cancer research.

[12]  T. Takeuchi,et al.  New antibiotics, bleomycin A and B. , 1966, The Journal of antibiotics.

[13]  M. Okino,et al.  Effects of a high-voltage electrical impulse and an anticancer drug on in vivo growing tumors. , 1987, Japanese journal of cancer research : Gann.