Detection of Salmonella typhimurium in fat free milk using a phage immobilized magnetoelastic sensor

Abstract A magnetoelastic sensor immobilized with bacteriophage for the detection of Salmonella typhimurium in fat free milk is described. The response of the magnetoelastic sensors can be measured in the absence of direct physical wire contacts to the sensor, making the possibility of real time and in vivo bio-detection systems more realistic. The sensor system reported in this article is a combination of two rapidly developing technologies: magnetoelastic materials as a transducer and filamentous bacteriophage as a replacement to traditional bioprobes such as antibodies. Upon exposure to the target pathogen, the response of the phage immobilized magnetoelastic sensor changed, which was quantified by the shift in the sensor's resonance frequency. These frequency changes were proportional to the amount of target antigen bound to the sensor. Response of the sensors to increasing concentrations (5 × 10 1 to 5 × 10 8  cfu/ml) of S. typhimurium in two different liquid media (water and fat-free milk) was studied on sensors with dimensions 2 mm × 0.4 mm × 0.015 mm. Scanning electron microscopy (SEM) micrographics of previously assayed biosensors were used for visual correlation of sensor response. A detection limit of 5 × 10 3  cfu/ml, with a sensitivity of 159 Hz/decade was obtained for the sensors tested in water samples, as compared to 118 Hz/decade in fat free milk. The dissociation constant K d and the binding valencies for S. typhimurium spiked water samples was 136, 2.4 and 149 and 2.5 cfu/ml for spiked fat free milk samples.

[1]  B. Chin,et al.  Rapid and sensitive biosensor for Salmonella. , 2000, Biosensors & bioelectronics.

[2]  G. Herzer.  Magnetoelastic Sensors for Electronic Article Surveillance , 2007 .

[3]  Dmitri Ivnitski,et al.  Biosensors for detection of pathogenic bacteria , 1999 .

[4]  B. Brooks,et al.  Evaluation of culture enrichment procedures for use with Salmonella detection immunoassay. , 1999, International journal of food microbiology.

[5]  S. Singh,et al.  Recognition specificity of monoclonal antibodies which protect mice against Salmonella typhimurium infection. , 1999, Research in microbiology.

[6]  Craig A. Grimes,et al.  Measurement of temperature and liquid viscosity using wireless magneto-acoustic/magneto-optical sensors , 2001 .

[7]  A. Naugle,et al.  Estimate of Illnesses from Salmonella Enteritidis in Eggs, United States, 2000 , 2005, Emerging infectious diseases.

[8]  E. Reynisson,et al.  Evaluation of probe chemistries and platforms to improve the detection limit of real-time PCR. , 2006, Journal of microbiological methods.

[9]  Craig A. Grimes,et al.  A salt-independent pH sensor , 2001 .

[10]  Craig A. Grimes,et al.  The frequency response of magnetoelastic sensors to stress and atmospheric pressure , 2000 .

[11]  Giyoung Kim,et al.  Antibody Immobilization on Waveguides Using a Flow–Through System Shows Improved Listeria monocytogenes Detection in an Automated Fiber Optic Biosensor: RAPTOR™ , 2006, Sensors (Basel, Switzerland).

[12]  M. Wagner,et al.  Real-time PCR for the detection of Salmonella spp. in food: An alternative approach to a conventional PCR system suggested by the FOOD-PCR project. , 2006, Journal of microbiological methods.

[13]  Shaohua Zhao,et al.  A PCR-ELISA for detecting Shiga toxin-producing Escherichia coli. , 2002, Microbes and infection.

[14]  Craig A. Grimes,et al.  Magnetoacoustic remote query temperature and humidity sensors , 2000 .

[15]  Valery A Petrenko,et al.  Detection of biological threats. A challenge for directed molecular evolution. , 2004, Journal of microbiological methods.

[16]  Kefeng Zeng,et al.  Magnetoelastic immunosensors: amplified mass immunosorbent assay for detection of Escherichia coli O157:H7. , 2003, Analytical chemistry.

[17]  C A Grimes,et al.  A remote query magnetostrictive viscosity sensor. , 2000, Sensors and actuators. A, Physical.

[18]  C A Grimes,et al.  Remote query measurement of pressure, fluid-flow velocity, and humidity using magnetoelastic thick-film sensors. , 2000, Sensors and actuators. A, Physical.

[19]  P. Ripka,et al.  Gastric motility and volume sensing by implanted magnetic sensors , 2007 .

[20]  I-Hsuan Chen,et al.  Landscape phage probes for Salmonella typhimurium. , 2005, Journal of microbiological methods.

[21]  K R Rogers,et al.  Recent advances in biosensor techniques for environmental monitoring. , 2006, Analytica chimica acta.

[22]  B. Chin,et al.  Specific and selective biosensor for Salmonella and its detection in the environment. , 2003, Journal of microbiological methods.

[23]  Craig A. Grimes,et al.  Remote Query Resonant-Circuit Sensors for Monitoring of Bacteria Growth: Application to Food Quality Control , 2002 .

[24]  Olivier Lazcka,et al.  Pathogen detection: a perspective of traditional methods and biosensors. , 2007, Biosensors & bioelectronics.

[25]  D. Lim,et al.  Rapid detection of Bacillus anthracis spores directly from powders with an evanescent wave fiber-optic biosensor. , 2004, Journal of microbiological methods.

[26]  Jong-Seok Kim,et al.  Simultaneous detection by PCR of Escherichia coli, Listeria monocytogenes and Salmonella typhimurium in artificially inoculated wheat grain. , 2006, International journal of food microbiology.

[27]  V. Petrenko,et al.  Thermostability of landscape phage probes , 2005, Analytical and bioanalytical chemistry.

[28]  S. Timoshenko,et al.  Theory of elasticity , 1975 .

[29]  G. P. Smith,et al.  A library of organic landscapes on filamentous phage. , 1996, Protein engineering.

[30]  M. Vázquez,et al.  Multifunctional Magnetoelastic Sensor Device Based in Multilayer Magnetic Microwires , 2007 .

[31]  Quantification of ricin concentrations in aqueous media , 2005 .

[32]  B. Chin,et al.  Magnetoelastic biosensor for the detection of Salmonella typhimurium in food products , 2007 .

[33]  R. Cernosek,et al.  Blood rheological characterization using the thickness-shear mode resonator. , 2004, Biosensors & bioelectronics.

[34]  Kefeng Zeng,et al.  A rapid highly-sensitive endotoxin detection system. , 2006, Biosensors & bioelectronics.

[35]  K G Ong,et al.  Monitoring of bacteria growth using a wireless, remote query resonant-circuit sensor: application to environmental sensing. , 2001, Biosensors & bioelectronics.

[36]  Valery A Petrenko,et al.  Phage display for detection of biological threat agents. , 2003, Journal of microbiological methods.

[37]  Valery A Petrenko,et al.  Phage as a molecular recognition element in biosensors immobilized by physical adsorption. , 2007, Biosensors & bioelectronics.

[38]  B. Chin,et al.  A magnetoelastic resonance biosensor immobilized with polyclonal antibody for the detection of Salmonella typhimurium. , 2007, Biosensors & bioelectronics.

[39]  Craig A. Grimes,et al.  The real-time, in situ monitoring of calcium oxalate and brushite precipitation using magnetoelastic sensors , 2005 .

[40]  Jiří Homola,et al.  Detection of foodborne pathogens using surface plasmon resonance biosensors , 2001 .