Automated data massaging, interpretation, and e-mailing modules for high throughput open access mass spectrometry

Hardware components and software modules were configured to enhance the automation, efficiency, and reliability of a commercial open access atmospheric pressure ionization mass spectrometry (API/MS) system for flow injection analysis. The data massaging module is a versatile package for data manipulation/reduction which is initialized upon detecting the end of data acquisition and can function in parallel during the data acquisition of the next sample. The data interpretation module compares the ions in the acquired mass spectrum with the predicted molecular adduct ions in different charge states, as well as the predicted isotopic distributions, possible artifact, polymer/cluster, byproduct/fragmentation ions, and then uses the results to score the quality of the spectrum. The e-mailing module transmits the spectrum and interpretation report to the desktop computer of the submitting chemist where the spectrum can be displayed and the report viewed. A scheme is also presented for the automated interpretation of an API mass spectrum for the determination of the most likely molecular weights of the components present in an “unknown” sample. Related flow diagrams, algorithms, and applications are illustrated.

[1]  E Görlach,et al.  High-throughput flow injection analysis mass spectroscopy with networked delivery of color-rendered results. 2. Three-dimensional spectral mapping of 96-well combinatorial chemistry racks. , 1998, Analytical chemistry.

[2]  J. Seifert,et al.  High-throughput flow injection analysis–mass spectrometry with networked delivery of colour rendered results: the characterisation of liquid chromatography fractions , 1999 .

[3]  M. Siegel,et al.  Evaluation of a dual electrospray ionization/atmospheric pressure chemical ionization source at low flow rates (∼50 µL/min) for the analysis of both highly and weakly polar compounds , 1998 .

[4]  Rapid methods for screening low molecular mass compounds non-covalently bound to proteins using size exclusion and mass spectrometry applied to inhibitors of human cytomegalovirus protease. , 1998, Journal of mass spectrometry : JMS.

[5]  K. Biemann,et al.  A computer-compatible digital data acquisition system for fast-scanning, single-focusing mass spectrometers , 1967 .

[6]  F. Pullen,et al.  Putting mass spectrometry in the hands of the end user , 1995, Journal of the American Society for Mass Spectrometry.

[7]  Frank H. Laukien,et al.  Automation of a Fourier transform ion cyclotron resonance mass spectrometer for acquisition, analysis, and e-mailing of high-resolution exact-mass electrospray ionization mass spectral data , 1999 .

[8]  L. Taylor,et al.  Open access atmospheric pressure chemical ionization Mass spectrometry for routine sample analysis , 1995, Journal of the American Society for Mass Spectrometry.