Nucleotide sequence and exon-intron structure of the bovine transition protein 1 gene.

The nucleotide sequence and exon-intron structure of the bovine transition protein 1 gene was determined. It consists of 2 exons (E1, 139 bp; E2, 29 bp) and a single intron (220 bp). The position of the transcription initiation site was determined 30 nucleotides upstream of ATG. TAAATA- and CAAT-boxes were found 60 and 121 bp upstream of the ATG-translation start point, respectively. It was observed that transition protein 1 is highly conserved in mammals at the nucleotide as well as at the amino acid level.