Integrative Technology for the Twenty‐First Century

Abstract: Presented is the concept of Integrative Technology, the intersection of the precision assembly of matter (nanotechnology), coupled with the functional building blocks of nature (biotechnology), and fused by the network flow of spatiotemporal information (informatics). The power of Integrative Technology is illuminated through an illustrative example; the engineering of nano‐sized excitable vesicles with the ability to intrinsically process information. The fusion of the tools of nanotechnology and biotechnology to produce excitable vesicles is described, as is the mechanics of information flow that ultimately lead to the manifestations of emergent higher‐order behavior. Finally, the potential of using systems engineered and produced from nanoscale components to create complex systems and materials that manifest embedded functional behavior is discussed.

[1]  Liang Liang,et al.  Templateless assembly of molecularly aligned conductive polymer nanowires: a new approach for oriented nanostructures. , 2003, Chemistry.

[2]  Loren L Looger,et al.  Control of a biomolecular motor-powered nanodevice with an engineered chemical switch , 2002, Nature materials.

[3]  Shoeb Ahmad,et al.  Post-translational integration and oligomerization of connexin 26 in plasma membranes and evidence of formation of membrane pores: implications for the assembly of gap junctions. , 2002, The Biochemical journal.

[4]  A. Graff,et al.  Virus-assisted loading of polymer nanocontainer , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[5]  P. Ciancaglini,et al.  Solubilization of Na,K-ATPase from rabbit kidney outer medulla using only C12E8. , 2002, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.

[6]  Mathias Winterhalter,et al.  Amphiphilic block copolymer nanocontainers as bioreactors , 2001 .

[7]  J J Hopfield,et al.  What is a moment? Transient synchrony as a collective mechanism for spatiotemporal integration. , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[8]  F. Bukauskas Inducing de novo formation of gap junction channels. , 2001, Methods in molecular biology.

[9]  Thomas Hirt,et al.  Polymerized ABA Triblock Copolymer Vesicles , 2000 .

[10]  C. H. George,et al.  Synthesis and assembly of connexins in vitro into homomeric and heteromeric functional gap junction hemichannels. , 1999, The Biochemical journal.

[11]  P. A. Pedersen,et al.  High Yield Fermentation of Pig α1β1 Na,K‐ATPase in Saccharomyces cerevisiae , 1997 .

[12]  D. G. Morris,et al.  Role of conducting polymeric interfaces in promoting biological electron transfer. , 1997, Biosensors & bioelectronics.

[13]  A. Villalobo,et al.  Reconstitution of rat liver gap junctions into liposomes. , 1994, Biochemical Society transactions.

[14]  E. Perozo,et al.  Purification and reconstitution of functional Shaker K+ channels assayed with a light-driven voltage-control system. , 1994, Biochemistry.

[15]  P. A. Pedersen,et al.  Expression of Na,K‐ATPase in Saccharomyces cerevisiae , 1992, Annals of the New York Academy of Sciences.

[16]  C. Y. Chen,et al.  Synthesis and assembly of functional mammalian Na,K-ATPase in yeast. , 1990, The Journal of biological chemistry.

[17]  W. Agnew,et al.  Affinity purification of the voltage-sensitive sodium channel from electroplax with resins selective for sialic acid. , 1989, Biochemistry.

[18]  B. Spooner,et al.  Reconstitution of cardiac gap junction channeling activity into liposomes: a functional assay for gap junctions. , 1988, Biochemical and biophysical research communications.

[19]  P. L. Jørgensen Purification of Na+,K+-ATPase: enzyme sources, preparative problems, and preparation from mammalian kidney. , 1988, Methods in enzymology.

[20]  F. Cornelius Incorporation of C12E8-solubilized Na+,K+-ATPase into liposomes: determination of sidedness and orientation. , 1988, Methods in enzymology.