Optimization and Scale Up of Cellulase-Free Xylanase Production in Solid State Fermentation on Wheat Bran by Cellulosimicrobium sp. MTCC 10645

The production of cellulase-free xylanase was investigated with a locally isolated and identified strain, Cellulosimicrobium sp. MTCC 10645 in solid state fermentation. Different fermentation conditions were standardised for the growth and xylanase activity, the optimum being 72 h growth at pH 7.0, cultivation temperature 40°C and substrate to moisture ratio of 1:1.8 (w/v). Among different lignocellulosic substrates wheat bran was found to be best substrate for xylanase production. The inoculum size of 10% resulted in maximum production of xylanase. The enzyme production was stimulated by the addition of yeast extract and casein. Addition of glucose, xylose and carboxy methyl cellulose repressed the production of xylanase. Under optimized conditions, xylanase production in solid state fermentation was 4,962 + 45.08 U/gds.

[1]  Isolation, purification and characterization of the xylanase produced by Arthrobacter sp. in solid-state cultivation , 2014 .

[2]  U. Gupta,et al.  Optimization and Scale up of Cellulase free Endo xylanase Production by Solid State Fermentation on Corn cob and by Immobilized Cells of a Thermotolerant Bacterial Isolate , 2008 .

[3]  R. C. Kuhad,et al.  Enhanced production of cellulase-free thermostable xylanase by Bacillus pumilus ASH and its potential application in paper industry , 2007 .

[4]  R. C. Kuhad,et al.  High-level xylanase production by alkaliphilic Bacillus pumilus ASH under solid-state fermentation , 2006 .

[5]  N. Bhosle,et al.  Isolation, purification and characterization of the xylanase produced by Arthrobacter sp. MTCC 5214 when grown in solid-state fermentation , 2006 .

[6]  Prince Sharma,et al.  Production of Cellulase-Free Xylanase from Bacillus megaterium by Solid State Fermentation for Biobleaching of Pulp , 2006, Current Microbiology.

[7]  J. A. Jorge,et al.  Xylanases from fungi: properties and industrial applications , 2005, Applied Microbiology and Biotechnology.

[8]  M. Ayub,et al.  Optimization of cellulase-free xylanase activity produced by Bacillus coagulans BL69 in solid-state cultivation , 2005 .

[9]  L. Ten,et al.  Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates. , 2004, Journal of microbiological methods.

[10]  M. Raimbault,et al.  Culture method to study fungal growth in solid fermentation , 1980, European journal of applied microbiology and biotechnology.

[11]  K. Sasaki,et al.  Thermostable and alkaline-tolerant microbial cellulase-free xylanases produced from agricultural wastes and the properties required for use in pulp bleaching bioprocesses: a review , 2003 .

[12]  Amare Gessesse,et al.  High-level xylanase production by an alkaliphilic Bacillus sp. by using solid-state fermentation , 1999 .

[13]  T. Satyanarayana,et al.  Xylanase production by thermophilic Bacillus licheniformis A99 in solid-state fermentation , 1997 .

[14]  R. Pérez,et al.  β‐Xylosidase and xylanase characterization and production by Streptomyces sp. CH‐M‐1035 , 1997 .

[15]  T. Satyanarayana,et al.  α-Amylase production by thermophilic Bacillus coagulans in solid state fermentation , 1995 .

[16]  D. Mitchell,et al.  Protease production by Rhizopus oligosporus in solid-state fermentation , 1994, World journal of microbiology & biotechnology.

[17]  R. Kellaway,et al.  Botanical composition and extent of lignification affecting digestibility of wheat and oat straw and paspalum hay , 1982 .

[18]  M. Moo-young,et al.  Interlaboratory testing of methods for assay of xylanase activity , 2002 .