Highly sensitive and selective erythromycin nanosensor employing fiber optic SPR/ERY imprinted nanostructure: Application in milk and honey.

An erythromycin (ERY) detection method is proposed using the fiber optic core decorated with the coatings of silver and an over layer of ERY imprinted nanoparticles. Synthesis of ERY imprinted nanoparticles is carried out using miniemulsion method. The operating range of the sensor is observed to be from 1.62×10-3 to 100µM while the sensor possesses the linear response for ERY concentration range from 0.1 to 5µM. The sensing method shows a maximum sensitivity of 205nm/µM near ERY concentration of 0.01µM. The detection limit and the quantification limit of the sensor are found to be 1.62×10-3µM and 6.14×10-3µM, respectively. The sensor's applicability in real samples is also examined and is found to be in good agreement for the industrial application. The sensor possesses numerous advantages like fast response time (<15s), simple, low cost, highly selective along with abilities towards online monitoring and remote sensing of analyte.

[1]  Jian Wang,et al.  Analyses of macrolide antibiotic residues in eggs, raw milk, and honey using both ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and high-performance liquid chromatography/tandem mass spectrometry. , 2007, Rapid communications in mass spectrometry : RCM.

[2]  L. Uzun,et al.  Magnetic Nanoparticles for Plasmid DNA Purification through Hydrophobic Interaction Chromatography , 2014 .

[3]  G. Palleschi,et al.  Analysis of erythromycin and tylosin in bovine muscle using disposable screen printed electrodes. , 2004, The Analyst.

[4]  Peter A. Lieberzeit,et al.  Molecularly imprinted polymer nanoparticles in chemical sensing – Synthesis, characterisation and application , 2015 .

[5]  S. Prasad,et al.  An Electrochemical Sensor for the Detection of Antibiotic Contaminants in Water UTD AUTHOR ( S ) : , 2014 .

[6]  Adil Denizli,et al.  Protein recognition via ion-coordinated molecularly imprinted supermacroporous cryogels. , 2008, Journal of chromatography. A.

[7]  Yan-Chun Feng,et al.  Construction of universal quantitative models for determination of roxithromycin and erythromycin ethylsuccinate in tablets from different manufacturers using near infrared reflectance spectroscopy. , 2006, Journal of pharmaceutical and biomedical analysis.

[8]  Jie Li,et al.  Electrochemical sensor based on gold nanoparticles fabricated molecularly imprinted polymer film at chitosan-platinum nanoparticles/graphene-gold nanoparticles double nanocomposites modified electrode for detection of erythromycin. , 2012, Biosensors & bioelectronics.

[9]  L. Aydın,et al.  Erythromycin residue in honey from the Southern Marmara region of Turkey , 2008, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[10]  B. D. Gupta,et al.  Simultaneous estimation of vitamin K1 and heparin with low limit of detection using cascaded channels fiber optic surface plasmon resonance. , 2016, Biosensors & bioelectronics.

[11]  Banshi D. Gupta,et al.  Surface plasmon resonance based fiber optic ethanol sensor using layers of silver/silicon/hydrogel entrapped with ADH/NAD , 2016 .

[12]  T. S. Thompson,et al.  Degradation of erythromycin in honey and selection of suitable marker residues for food safety analysis , 2012 .

[13]  E. Elkady,et al.  Liquid chromatographic and spectrophotometric methods for the determination of erythromycin stearate and trimethoprim in tablets , 2011 .

[14]  Banshi D. Gupta,et al.  Surface Plasmon Resonance-Based Fiber Optic Sensor for the Detection of Ascorbic Acid Utilizing Molecularly Imprinted Polyaniline Film , 2015, Plasmonics.

[15]  Ibrahim Abdulhalim,et al.  Electromagnetic fields distribution in multilayer thin film structures and the origin of sensitivity enhancement in surface plasmon resonance sensors , 2010 .

[16]  B. Zhang,et al.  Enhanced electrochemical detection of erythromycin based on acetylene black nanoparticles. , 2010, Colloids and surfaces. B, Biointerfaces.

[17]  Noriaki Hara,et al.  SPR sensor chip for detection of small molecules using molecularly imprinted polymer with embedded gold nanoparticles. , 2005, Analytical chemistry.

[18]  J. Plaizier-Vercammen,et al.  Investigation on the chemical stability of erythromycin in solutions using an optimization system , 1996, Pharmacy World and Science.

[19]  R. N. Shah,et al.  Characterization of molecularly imprinted polymers with the Langmuir-Freundlich isotherm. , 2001, Analytical chemistry.

[20]  A. Denizli,et al.  Preconcentration of copper using double-imprinted polymer via solid phase extraction , 2006 .

[21]  R. Carman,et al.  Antibiotics in the human food chain: establishing no effect levels of tetracycline, neomycin, and erythromycin using a chemostat model of the human colonic microflora. , 2005, Regulatory toxicology and pharmacology : RTP.

[22]  R. Gutiérrez,et al.  High-performance thin-layer chromatography-bioautography for multiple antibiotic residues in cow's milk. , 2003, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[23]  Banshi D. Gupta,et al.  Surface Plasmon Resonance-Based Fiber Optic Sensors Utilizing Molecular Imprinting , 2016, Sensors.

[24]  J. Homola.  Present and future of surface plasmon resonance biosensors , 2003, Analytical and bioanalytical chemistry.

[25]  Yong‐Ill Lee,et al.  Selective and sensitive determination of erythromycin in honey and dairy products by molecularly imprinted polymers based electrochemical sensor , 2014 .

[26]  A. Laganà,et al.  A simple and rapid confirmatory assay for analyzing antibiotic residues of the macrolide class and lincomycin in bovine milk and yoghurt: hot water extraction followed by liquid chromatography/tandem mass spectrometry. , 2007, Rapid communications in mass spectrometry : RCM.

[27]  Haijia Su,et al.  Preparation, characterization and adsorption performance of molecularly imprinted microspheres for erythromycin using suspension polymerization , 2012 .

[28]  Shoufang Xu,et al.  Recent advances in molecular imprinting technology: current status, challenges and highlighted applications. , 2011, Chemical Society reviews.

[29]  A. Denizli,et al.  Fabrication of surface plasmon resonance nanosensor for the selective determination of erythromycin via molecular imprinted nanoparticles. , 2016, Talanta.

[30]  Banshi D. Gupta,et al.  Surface Plasmon Resonance-Based Fiber Optic Sensors: Principle, Probe Designs, and Some Applications , 2009, J. Sensors.

[31]  P. Cormack,et al.  Molecularly imprinted polymers: synthesis and characterisation. , 2004, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[32]  Banshi D Gupta,et al.  A contemporary approach for design and characterization of fiber-optic-cortisol sensor tailoring LMR and ZnO/PPY molecularly imprinted film. , 2017, Biosensors & bioelectronics.

[33]  Adil Denizli,et al.  Quartz crystal microbalance based nanosensor for lysozyme detection with lysozyme imprinted nanoparticles. , 2010, Biosensors & bioelectronics.