Transient receptor potential channels in the inner ear: presence of transient receptor potential channel subfamily 1 and 4 in the guinea pig inner ear

Conclusion. The results of this study indicate that transient receptor potential subfamily 1 (TRPV1) may play a functional role in sensory cell physiology and that TRPV4 may be important for fluid homeostasis in the inner ear. Objective. To analyze the expression of TRPV1 and -4 in the normal guinea pig inner ear. Material and methods. Albino guinea pigs were used. The location of TRPV1 and -4 in the inner ear, i.e. cochlea, vestibular end organs and endolymphatic sac, was investigated by means of immunohistochemistry. Results. Immunohistochemistry revealed the presence of TRPV1 in the hair cells and supporting cells of the organ of Corti, in spiral ganglion cells, sensory cells of the vestibular end organs and vestibular ganglion cells. TRPV4 was found in the hair cells and supporting cells of the organ of Corti, in marginal cells of the stria vascularis, spiral ganglion cells, sensory cells, transitional cells, dark cells in the vestibular end organs, vestibular ganglion cells and epithelial cells of the endolymphatic sac.

[1]  K. Matsunaga,et al.  Immunoreactivity of VR1 on epidermal keratinocyte of human skin. , 2001, Biochemical and biophysical research communications.

[2]  L. Vyklický,et al.  Inflammatory mediators at acidic pH activate capsaicin receptors in cultured sensory neurons from newborn rats. , 1998, Journal of neurophysiology.

[3]  Simon C Watkins,et al.  Vanilloid receptor expression suggests a sensory role for urinary bladder epithelial cells , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[4]  A. Hudspeth,et al.  Vanilloid Receptor–Related Osmotically Activated Channel (VR-OAC), a Candidate Vertebrate Osmoreceptor , 2000, Cell.

[5]  Y. Cazals,et al.  Guinea pig vestibular type I hair cells can show reversible shortening. , 1991, Journal of vestibular research : equilibrium & orientation.

[6]  Craig Montell,et al.  A unified nomenclature for the superfamily of TRP cation channels. , 2002, Molecular cell.

[7]  C. Tohda,et al.  Existence of capsaicin‐sensitive glutamatergic terminals in rat hypothalamus , 1998, Neuroreport.

[8]  Jianxun Zhou,et al.  Type 1 vanilloid receptor expression by mammalian inner ear ganglion cells , 2003, Hearing Research.

[9]  Jiefu Zheng,et al.  Vanilloid receptors in hearing: altered cochlear sensitivity by vanilloids and expression of TRPV1 in the organ of corti. , 2003, Journal of neurophysiology.

[10]  B. Nilius,et al.  TRPs Make Sense , 2003, The Journal of Membrane Biology.

[11]  Rachel C Brown,et al.  The vanilloid receptor family of calcium-permeable channels: molecular integrators of microenvironmental stimuli. , 2003, News in physiological sciences : an international journal of physiology produced jointly by the International Union of Physiological Sciences and the American Physiological Society.

[12]  B. Minke,et al.  TRP channel proteins and signal transduction. , 2002, Physiological reviews.

[13]  S. Koizumi,et al.  Functional vanilloid receptors in cultured normal human epidermal keratinocytes. , 2002, Biochemical and biophysical research communications.

[14]  D. Julius,et al.  The capsaicin receptor: a heat-activated ion channel in the pain pathway , 1997, Nature.

[15]  F. Bari,et al.  Possible involvement of capsaicin-sensitive sensory nerves in the regulation of cochlear blood flow in the guinea pig. , 1994, Acta oto-laryngologica.

[16]  L. Vyklický,et al.  An interaction of inflammatory mediators and protons in small diameter dorsal root ganglion neurons of the rat , 1997, Neuroscience Letters.

[17]  J. Aran,et al.  Osmotically induced motility of outer hair cells: implications for Menière's disease , 2004, Archives of oto-rhino-laryngology.

[18]  Makoto Tominaga,et al.  Heat-Evoked Activation of the Ion Channel, TRPV4 , 2002, The Journal of Neuroscience.

[19]  A. Nuttall,et al.  Nitric oxide mediates capsaicin-induced increase in cochlear blood flow , 1996, Hearing Research.

[20]  M. Caterina,et al.  Altered urinary bladder function in mice lacking the vanilloid receptor TRPV1 , 2002, Nature Neuroscience.

[21]  P. Blumberg,et al.  Distribution of mRNA for vanilloid receptor subtype 1 (VR1), and VR1-like immunoreactivity, in the central nervous system of the rat and human. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[22]  P. Blumberg,et al.  Vanilloid (Capsaicin) receptors and mechanisms. , 1999, Pharmacological reviews.