Binding Study of Phenformin with Bovine Serum Album Using a Combined Technique of Equilibrium Dialysis with Flow-Injection Chemiluminescence Detection

ABSTRACT The interaction between phenformin hydrochloride and bovine serum albumin (BSA) was investigated by the methods of chemiluminescence combined with equilibrium dialysis technique. A novel N-bromosuccinimide (NBS)–eosin Y (EY) chemiluminescence (CL) method was established for the determination of phenformin. The mechanism of this chemiluminescence system was proposed. Optimization studies were performed to determine the phenformin. Under the optimal conditions, the CL intensity was linear for a phenformin concentration over the range of 4.6 × 10−8 to 5.0 × 10−5 g/mL. The detection limit was 1.5 × 10−8 g/mL. The data obtained by the present equilibrium dialysis–CL system were analyzed using the Klotz plot and the Scatchard analysis. The results showed that the Klotz plot and the Scatchard plot are linear with good correlation coefficient, indicating that the phenformin has only one type of binding site on BSA. The binding parameters were the number of the binding sites n (1.02) and the estimated association constant K (2.66 × 104 L/mol). The chemiluminescence system combined with equilibrium dialysis developed in this work demonstrated its use for determination of interaction between drug and protein by using relatively simple instrument.

[1]  Xiaoli Hu,et al.  Resonance Rayleigh scattering method for the determination of polyvinylpyrrolidone using eosin Y as the probe , 2010 .

[2]  Xiaoli Hu,et al.  Fluorescence Quenching Reaction of Polyvinylpyrrolidone-Eosin Y System for the Determination of Polyvinylpyrrolidone , 2010, Journal of Fluorescence.

[3]  M. Karlsson,et al.  Characterization of drug-protein binding process by employing equilibrium sampling through hollow-fiber supported liquid membrane and Bjerrum and Scatchard plots. , 2008, Journal of pharmaceutical and biomedical analysis.

[4]  Hanwen Sun,et al.  Flow-injection-enhanced chemiluminescence method for the determination of four anticancer drugs , 2008 .

[5]  Paul S. Francis,et al.  Acidic potassium permanganate as a chemiluminescence reagent--a review. , 2007, Analytica chimica acta.

[6]  A. Ishida,et al.  Estimation of the membrane permeability of liposomes via use of eosin Y chemiluminescence catalysed by peroxidase encapsulated in liposomes. , 2007, Luminescence : the journal of biological and chemical luminescence.

[7]  Zhengjun Gong,et al.  Coupling microdialysis with flow-injection chemiluminescence detection for a protein-drug interaction study. , 2006, Journal of pharmaceutical and biomedical analysis.

[8]  D. S. Hage,et al.  Quantitative analysis of allosteric drug-protein binding by biointeraction chromatography , 2004, Nature Biotechnology.

[9]  Xiao-Feng Yang,et al.  Flow-injection chemiluminescence determination of dihydralazine sulfate based on hexacyanoferrate(III) oxidation sensitized by eosin Y. , 2004, Talanta.

[10]  Zhouping Wang,et al.  N-Bromosuccinimide-Fluorescein Based Sensitive Flow-Injection Chemiluminescence Determination of Phenformin , 2004, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[11]  Zhouping Wang,et al.  A flow-injection ultrafiltration sampling chemiluminescence system for on-line determination of drug–protein interaction , 2003, Analytical and bioanalytical chemistry.

[12]  N. Alizadeh,et al.  Drug-selective electrode for ketamine determination in pharmaceutical preparations and electrochemical study of drug with BSA. , 2002, Journal of pharmaceutical and biomedical analysis.

[13]  Baoxin Li,et al.  Flow-injection chemiluminescence detection for studying protein binding for drug with ultrafiltration sampling , 2002 .

[14]  A. Kojło,et al.  Use of amino-carbonyl reaction and chemiluminescence detection to the flow-injection determination of some amino acids. , 2001, Talanta.

[15]  Y. Huang,et al.  Flow-injection analysis chemiluminescence detection combined with microdialysis sampling for studying protein binding of drug. , 2001, Talanta.

[16]  Y. Huang,et al.  A flow injection chemiluminescence system for the determination of isoniazid , 2000, Fresenius' journal of analytical chemistry.

[17]  D. S. Hage,et al.  High-performance affinity chromatography and immobilized serum albumin as probes for drug- and hormone-protein binding. , 2000, Journal of chromatography. B, Biomedical sciences and applications.

[18]  P. Houghton,et al.  Interaction of substituted phenoxazine chemosensitizers with bovine serum albumin. , 1999, Journal of pharmaceutical and biomedical analysis.

[19]  Yukihiro Kuroda,et al.  Development of high-performance frontal analysis and the application to the study of drug–plasma protein binding , 1999 .

[20]  S. Hassan,et al.  Determination of metformin in pharmaceutical preparations using potentiometry, spectrofluorimetry and UV-visible spectrophotometry , 1999 .

[21]  D. S. Hage,et al.  Recent advances in chromatographic and electrophoretic methods for the study of drug-protein interactions. , 1997, Journal of chromatography. B, Biomedical sciences and applications.