First application of loop‐mediated isothermal amplification (LAMP) assays for rapid identification of mating type in the heterothallic fungus Aspergillus fumigatus

Loop‐mediated isothermal amplification (LAMP) assays, which operate at a single temperature and require no postreaction processing, have been described for rapid species‐specific detection of numerous fungi. The technology has much less commonly been applied to identification of other key genetic traits such as fungicide resistance, and has not yet been applied to mating‐type determination in any fungus.

[1]  Jesus Rodriguez-Manzano,et al.  Rapid and Sensitive Detection of Azole-Resistant Aspergillus fumigatus by Tandem Repeat Loop-Mediated Isothermal Amplification. , 2019, The Journal of molecular diagnostics : JMD.

[2]  P. Dyer,et al.  The novel Aspergillus fumigatus MAT1-2-4 mating-type gene is required for mating and cleistothecia formation. , 2017, Fungal genetics and biology : FG & B.

[3]  B. Zwaan,et al.  A Novel Environmental Azole Resistance Mutation in Aspergillus fumigatus and a Possible Role of Sexual Reproduction in Its Emergence , 2017, mBio.

[4]  Li Han,et al.  Development and Evaluation of a Loop-Mediated Isothermal Amplification Method for Rapid Detection of Aspergillus fumigatus , 2016, Journal of Clinical Microbiology.

[5]  P. Dyer,et al.  Discovery of a Sexual Cycle in Aspergillus lentulus, a Close Relative of A. fumigatus , 2013, Eukaryotic Cell.

[6]  Birgit Hoff,et al.  Sexual reproduction and mating-type–mediated strain development in the penicillin-producing fungus Penicillium chrysogenum , 2013, Proceedings of the National Academy of Sciences.

[7]  A. Gross,et al.  Reproductive mode and life cycle of the ash dieback pathogen Hymenoscyphus pseudoalbidus. , 2012, Fungal genetics and biology : FG & B.

[8]  N. Keller,et al.  Pathogenesis of Aspergillus fumigatus in Invasive Aspergillosis , 2009, Clinical Microbiology Reviews.

[9]  P. Dyer,et al.  Discovery of a sexual cycle in the opportunistic fungal pathogen Aspergillus fumigatus , 2009, Nature.

[10]  J. Latgé,et al.  Evidence for Sexuality in the Opportunistic Fungal Pathogen Aspergillus fumigatus , 2005, Current Biology.

[11]  G. Kema,et al.  Isolation and characterization of the mating-type idiomorphs from the wheat septoria leaf blotch fungus Mycosphaerella graminicola. , 2002, Fungal genetics and biology : FG & B.

[12]  T. Notomi,et al.  Loop-mediated isothermal amplification of DNA. , 2000, Nucleic acids research.

[13]  P. Inderbitzin,et al.  14 Mating-Type Structure, Function, Regulation and Evolution in the Pezizomycotina , 2016 .

[14]  B. McDonald,et al.  Pathogen population genetics, evolutionary potential, and durable resistance. , 2002, Annual review of phytopathology.