Fabrication and characterization of a biomimetic composite scaffold for bone defect repair.

For successful bone tissue engineering, scaffolds with tailored properties are a basic requirement. The combination of different available materials not only appears to be desirable but also very challenging. In this study, a composite material consisting of hydroxyapatite and collagen was produced by a biomimetic precipitation method and characterized by X-ray diffraction (XRD) and thermogravimetry (TGA). Subsequently, a suspension-quick-freezing and lyophilization method was used to incorporate the hydroxyapatite into a polymeric matrix consisting of collagen and chitosan. Before physicochemical characterization, the highly porous scaffolds were consolidated by a dehydrothermal treatment (DHT). The main attention was focused on the particle size of hydroxyapatite, which should be in the nanometer range. This is relevant to achieve a homogeneous resorption of the material by osteoclasts. Small-angle X-ray scattering (SAXS), atomic force microscopy (AFM), and environmental scanning electron microscopy (ESEM) were used to evaluate the outcome. The results suggest a successful polymeric embedding of nanoscaled hydroxyapatite particles into the matrix of the spongy construct. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010.

[1]  S. Ichinose,et al.  Self-organization mechanism in a bone-like hydroxyapatite/collagen nanocomposite synthesized in vitro and its biological reaction in vivo. , 2001, Biomaterials.

[2]  A. Persikov,et al.  Unstable molecules form stable tissues , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[3]  T. Chaki,et al.  Sintering behaviour and mechanical properties of hydroxyapatite and dicalcium phosphate , 1993 .

[4]  X. D. Zhu,et al.  Bone regeneration by using scaffold based on mineralized recombinant collagen. , 2008, Journal of biomedical materials research. Part B, Applied biomaterials.

[5]  S. Kalita,et al.  Nanocrystalline hydroxyapatite doped with magnesium and zinc: Synthesis and characterization , 2007 .

[6]  Zhi Ding,et al.  Porous chitosan-gelatin scaffold containing plasmid DNA encoding transforming growth factor-beta1 for chondrocytes proliferation. , 2006, Biomaterials.

[7]  D. Hulmes.  Collagen Diversity, Synthesis and Assembly , 2008 .

[8]  Z. Xiong,et al.  Development of a porous poly(L-lactic acid)/hydroxyapatite/collagen scaffold as a BMP delivery system and its use in healing canine segmental bone defect. , 2003, Journal of biomedical materials research. Part A.

[9]  J. Howard,et al.  Assembly of collagen into microribbons: effects of pH and electrolytes. , 2004, Journal of structural biology.

[10]  V. Podrazký,et al.  Densities of collagen dehydrated by some organic solvents , 1966, Experientia.

[11]  R. I. Burlakov,et al.  Range of equipment for the anesthesiologist working in the operating theater , 1981 .

[12]  J T Czernuszka,et al.  Collagen-hydroxyapatite composites for hard tissue repair. , 2006, European cells & materials.

[13]  Wonmuk Hwang,et al.  Region‐specific role of water in collagen unwinding and assembly , 2008, Proteins.

[14]  Elliot P. Douglas,et al.  Bone structure and formation: A new perspective , 2007 .

[15]  J. Koenig.  Fourier transform infrared spectroscopy of polymers , 1984 .

[16]  A. Domard,et al.  Collagen and its interactions with chitosan, III some biological and mechanical properties. , 1996, Biomaterials.

[17]  G. W. Marshall,et al.  Collagen Orientation and Crystallite Size in Human Dentin: A Small Angle X-ray Scattering Study , 2001, Calcified Tissue International.

[18]  Y. Tabata,et al.  Repairing of rabbit skull defect by TGF-β1-incorporated collagen sponges of different thickness , 2003 .

[19]  Y. Koyama,et al.  Implantation study of a novel hydroxyapatite/collagen (HAp/col) composite into weight-bearing sites of dogs. , 2002, Journal of biomedical materials research.

[20]  A. Ruys,et al.  Sintering effects on the strength of hydroxyapatite. , 1995, Biomaterials.

[21]  Linda G Griffith,et al.  Engineering principles of clinical cell-based tissue engineering. , 2004, The Journal of bone and joint surgery. American volume.

[22]  V. Aswal,et al.  Surface patch binding induced intermolecular complexation and phase separation in aqueous solutions of similarly charged gelatin-chitosan molecules. , 2007, Journal of Physical Chemistry B.

[23]  Licheng Sun,et al.  Determination of EDTA species in water by second-derivative square-wave voltammetry using a chitosan-coated glassy carbon electrode. , 2003, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[24]  Yan Li,et al.  Self-assembly of mineralized collagen composites , 2007 .

[25]  M. McKee,et al.  Molecular Regulation of Osteoblast Differentiation , 2004 .

[26]  M. Barbosa,et al.  Engineering endochondral bone: in vivo studies. , 2009, Tissue engineering. Part A.

[27]  C. Kennedy,et al.  Molecular interactions in collagen and chitosan blends. , 2004, Biomaterials.

[28]  Masanori Kikuchi,et al.  Biomimetic synthesis of bone-like nanocomposites using the self-organization mechanism of hydroxyapatite and collagen , 2004 .

[29]  P. Brown,et al.  Biodegradable hydroxyapatite - Polymer composites , 2001 .

[30]  K. Yao,et al.  Modulation of nano-hydroxyapatite size via formation on chitosan-gelatin network film in situ. , 2007, Biomaterials.

[31]  A. Domard,et al.  Relation between the physicochemical characteristics of collagen and its interactions with chitosan: I. , 1993, Biomaterials.

[32]  Jiawei Wang,et al.  Novel chitosan/collagen scaffold containing transforming growth factor-beta1 DNA for periodontal tissue engineering. , 2006, Biochemical and biophysical research communications.

[33]  Dhirendra S Katti,et al.  Growth factor-delivery systems for tissue engineering: a materials perspective , 2006, Expert review of medical devices.

[34]  Suming Li,et al.  Biodegradation of PLA/GA polymers: increasing complexity. , 1994, Biomaterials.

[35]  Seeram Ramakrishna,et al.  Aqueous mediated synthesis of bioresorbable nanocrystalline hydroxyapatite , 2005 .

[36]  A. Domard,et al.  Collagen and its interaction with chitosan. II. Influence of the physicochemical characteristics of collagen. , 1995, Biomaterials.

[37]  George E. Davis,et al.  Affinity of integrins for damaged extracellular matrix: αvβ3 binds to denatured collagen type I through RGD sites , 1992 .

[38]  K. Mäder,et al.  Characterizing molar mass distributions and molecule structures of different chitosans using asymmetrical flow field-flow fractionation combined with multi-angle light scattering. , 2008, International journal of pharmaceutics.

[39]  G. Michler,et al.  Nanocristalline Hydroxyapatite for Bone Repair , 2007 .

[40]  Yang-Jo Seol,et al.  Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials. , 2002, Journal of controlled release : official journal of the Controlled Release Society.

[41]  Daniel J. Muller,et al.  Creating ultrathin nanoscopic collagen matrices for biological and biotechnological applications. , 2007, Small.

[42]  J. Siepmann,et al.  PLGA-based drug delivery systems: importance of the type of drug and device geometry. , 2008, International journal of pharmaceutics.

[43]  F. Cui,et al.  Hierarchical Self-Assembly of Nano-Fibrils in Mineralized Collagen , 2003 .