Salmonella colonization and shedding patterns of hens inoculated via semen.

Two experiments were conducted to determine the effect of inseminating hens with semen contaminated with marker strains of Salmonella enteritidis and S. typhimurium. Eggs were collected and examined for salmonellae. In Expt. 1, 3/57 (5.3%) of eggs from S. enteritidis-challenged hens and 2/50 (4.0%) of eggs from S. typhimurium-challenged hens were contaminated with salmonellae. Salmonellae were recovered from only the shell. In Expt. 2, however, no eggs from S. typhimurium-challenged hens were contaminated; 2/73 (2.7%) of the eggs from S. enteritidis-challenged hens were contaminated. In Expt. 1, S. typhimurium was present in 30% of the oviducts and 20% of the ovaries; S. enteritidis was present in 20% of the oviducts and 20% of the ovaries. No salmonellae-containing oviducts or ovaries were found in either S. enteritidis- or S. typhimurium-challenged hens in Expt. 2. S. enteritidis and S. typhimurium were found in fecal samples taken 24 hr after insemination, but only S. enteritidis was fecally shed 7 days after insemination (20% in Expt. 1). Data indicate that semen may severe as a vehicle for transmission of salmonellae to the hen, which may result in sporadic production of contaminated eggs.

[1]  O. Pancorbo,et al.  Prevalence of Salmonella enteritidis and Other Serovars in Ovaries of Layer Hens at Time of Slaughter. , 1991, Journal of food protection.

[2]  N. Cox,et al.  Presence and impact of Salmonella contamination in commercial broiler hatcheries. , 1990, Poultry science.

[3]  R. Gast,et al.  Production of Salmonella enteritidis-contaminated eggs by experimentally infected hens. , 1990, Avian diseases.

[4]  W. Dubbert.  Assessment of Salmonella contamination in poultry--past, present, and future. , 1988, Poultry science.

[5]  J. Dunlop.  Salmonella infection in the community. , 1985, Public health.

[6]  G. R. McDaniel,et al.  Microbial flora observed within sections of the oviduct in naturally mated, artificially inseminated, and virgin hens. , 1978, Poultry science.

[7]  D. J. Fagerberg,et al.  Enrichment and plating methodology for Salmonella detection in food. A Review , 1976 .

[8]  N. Cox,et al.  Salmonella in the laying hen. 1. Salmonella recovery from viscera, feces and eggs following oral inoculation. , 1973, Poultry science.

[9]  R. Forsythe,et al.  Salmonellae recovery following gastro-intestinal and ovarian inoculation in the domestic fowl. , 1967, Poultry science.

[10]  F. H. Wilcox,et al.  The effect of antibiotics on bacteria in semen and on motility and fertilizing ability of chicken spermatozoa. , 1958, American journal of veterinary research.

[11]  N. Gibbons,et al.  A Note on Artificially Infected Fowl as Carriers of Salmonella , 1946 .

[12]  R. Axtell,et al.  ENVIRONMENTAL FACTORS CONTRIBUTING TO SALMONELLA COLONIZATION OF CHICKENS , 1991 .

[13]  M. Blaser,et al.  A review of human salmonellosis: III. Magnitude of Salmonella infection in the United States. , 1988, Reviews of infectious diseases.

[14]  W. R. Mitchell,et al.  An epidemiological study of Salmonellae in broiler chicken production. , 1980, Canadian journal of public health = Revue canadienne de sante publique.

[15]  E. Harry.  Some observations on the bacterial content of the ovary and oviduct of the fowl , 1963 .