Phase shifting white light interferometry using colour CCD for optical metrology and bio-imaging applications

Phase shifting white light interferometry (PSWLI) has been widely used for optical metrology applications because of their precision, reliability, and versatility. White light interferometry using monochrome CCD makes the measurement process slow for metrology applications. WLI integrated with Red-Green-Blue (RGB) CCD camera is finding imaging applications in the fields optical metrology and bio-imaging. Wavelength dependent refractive index profiles of biological samples were computed from colour white light interferograms. In recent years, whole-filed refractive index profiles of red blood cells (RBCs), onion skin, fish cornea, etc. were measured from RGB interferograms. In this paper, we discuss the bio-imaging applications of colour CCD based white light interferometry. The approach makes the measurement faster, easier, cost-effective, and even dynamic by using single fringe analysis methods, for industrial applications.

[1]  W. Marsden.  I and J , 2012 .

[2]  Paul Kumar Upputuri,et al.  White light single-shot interferometry with colour CCD camera for optical inspection of microsystems , 2015, International Conference on Optical and Photonic Engineering.

[3]  Manojit Pramanik,et al.  Microsphere-aided Optical Microscopy and its Applications for Super-Resolution Imaging , 2017 .

[4]  Jean-Michel Desse,et al.  Oil-film interferometry skin-friction measurement under white light , 2003 .

[5]  Tsuyoshi Murata,et al.  {m , 1934, ACML.

[6]  Dalip Singh Mehta,et al.  High-resolution corneal topography and tomography of fish eye using wide-field white light interference microscopy , 2013 .

[7]  Paul Kumar Upputuri,et al.  White light interferometer with color CCD for 3D-surface profiling of microsystems , 2015 .

[8]  Katsuichi Kitagawa.  Single-shot surface profiling by multiwavelength interferometry without carrier fringe introduction , 2012, J. Electronic Imaging.

[9]  Dalip Singh Mehta,et al.  Single shot white light interference microscopy with colour fringe analysis for quantitative phase imaging of biological cells , 2013, Photonics West - Biomedical Optics.

[10]  Paul Kumar Upputuri,et al.  Measurement of discontinuous surfaces using multiple-wavelength interferometry , 2009 .

[11]  Paul Kumar Upputuri,et al.  Multi-colour microscopic interferometry for optical metrology and imaging applications , 2016 .

[12]  Mahendra P. Kothiyal,et al.  Red-Green-Blue wavelength interferometry and TV holography for surface metrology , 2011 .

[13]  Anand Asundi,et al.  Microscopic TV holography for MEMS deflection and 3-D surface profile characterization , 2008 .

[14]  M P Kothiyal,et al.  Microscopic TV shearography for characterization of microsystems. , 2009, Optics letters.

[15]  Anand Asundi,et al.  Deformation and shape measurement using multiple wavelength microscopic TV holography , 2009 .

[16]  Mahendra P. Kothiyal,et al.  Single frame digital fringe projection profilometry for 3-D surface shape measurement , 2013 .

[17]  Xiaoyuan He,et al.  Single-shot color fringe projection for three-dimensional shape measurement of objects with discontinuities. , 2012, Applied optics.

[18]  Qingming Luo,et al.  Dual-wavelength laser speckle imaging to simultaneously access blood flow, blood volume, and oxygenation using a color CCD camera. , 2013, Optics letters.

[19]  Johannes Schwider,et al.  Red-green-blue interferometer for the metrology of discontinuous structures. , 2003, Applied optics.

[20]  Katsuichi Kitagawa.  Fast Surface Profiling by Multi-Wavelength Single-Shot Interferometry , 2010 .

[21]  Wang Haifeng,et al.  White light interferometry for surface profiling with a colour CCD , 2012 .

[22]  Katherine Creath,et al.  Dynamic four-dimensional microscope system with automated background leveling , 2012, Other Conferences.

[23]  Manojit Pramanik,et al.  Measurement of large discontinuities using single white light interferogram. , 2014, Optics express.

[24]  Sanjit K Debnath,et al.  Experimental study of the phase-shift miscalibration error in phase-shifting interferometry: use of a spectrally resolved white-light interferometer. , 2007, Applied optics.

[25]  U. Sennhauser,et al.  Multiwavelength shearography for quantitative measurements of two-dimensional strain distributions. , 1999, Applied optics.

[26]  Pascal Picart,et al.  Sensor influence in digital 3λ holographic interferometry , 2011 .

[27]  B. Maniscalco,et al.  Thin film thickness measurements using Scanning White Light Interferometry , 2014 .

[28]  M. P. Kothiyal,et al.  Multiple wavelength interferometry for surface profiling , 2008, Optical Engineering + Applications.

[29]  Mahendra P. Kothiyal,et al.  Two-wavelength micro-interferometry for 3-D surface profiling , 2009 .

[30]  Dalip Singh Mehta,et al.  Quantitative phase imaging of human red blood cells using phase-shifting white light interference microscopy with colour fringe analysis , 2012 .

[31]  R. Stephenson.  A and V , 1962, The British journal of ophthalmology.

[32]  Mahendra P. Kothiyal,et al.  Measurement of static and vibrating microsystems using microscopicTV holography , 2011 .

[33]  Tan H. Nguyen,et al.  Diffraction phase microscopy: principles and applications in materials and life sciences , 2014 .

[34]  Leslie L. Deck,et al.  Surface Profiling by Analysis of White-light Interferograms in the Spatial Frequency Domain , 1995 .