Annealing and purification of fluorescently labeled DNA substrates for in vitro assays
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[1] S. Sharpe,et al. Phosphorylation of the DNA repair scaffold SLX4 drives folding of the SAP domain and activation of the MUS81-EME1 endonuclease. , 2022, Cell reports.
[2] J. Lietard,et al. Sequence-dependence of Cy3 and Cy5 dyes in 3ʹ terminally-labeled single-stranded DNA , 2022, Scientific Reports.
[3] J. Lietard,et al. Sequence-dependent quenching of fluorescein fluorescence on single-stranded and double-stranded DNA , 2022, RSC advances.
[4] B. Payliss,et al. Exploring the Structures and Functions of Macromolecular SLX4-Nuclease Complexes in Genome Stability , 2021, Frontiers in Genetics.
[5] OUP accepted manuscript , 2021, Nucleic Acids Research.
[6] P. Sung,et al. Cooperativity of Mus81 (cid:1) Mms4 with Rad54 in the Resolution of Recombination and Replication Intermediates * □ , 2020 .
[7] M. Nowotny,et al. Recognition and processing of branched DNA substrates by Slx1–Slx4 nuclease , 2019, Nucleic acids research.
[8] S. West,et al. The SMX DNA Repair Tri-nuclease , 2017, Molecular cell.
[9] Miguel G. Blanco,et al. A Mechanism for Controlled Breakage of Under-replicated Chromosomes during Mitosis. , 2016, Developmental cell.
[10] M. Somoza,et al. Sequence-Dependent Fluorescence of Cy3- and Cy5-Labeled Double-Stranded DNA , 2016, Bioconjugate chemistry.
[11] Keith Roberts,et al. Molecular Cloning A Laboratory Manual Fourth Edition , 2015 .
[12] J. Rouse,et al. Identification and characterization of MUS81 point mutations that abolish interaction with the SLX4 scaffold protein , 2014, DNA repair.
[13] K. J. Patel,et al. Mouse SLX4 Is a Tumor Suppressor that Stimulates the Activity of the Nuclease XPF-ERCC1 in DNA Crosslink Repair , 2014, Molecular cell.
[14] M. Somoza,et al. Comparison of the Sequence-Dependent Fluorescence of the Cyanine Dyes Cy3, Cy5, DyLight DY547 and DyLight DY647 on Single-Stranded DNA , 2014, PloS one.
[15] S. West,et al. Coordinated actions of SLX1-SLX4 and MUS81-EME1 for Holliday junction resolution in human cells. , 2013, Molecular cell.
[16] S. López-Gomollón,et al. Purification of DNA Oligos by denaturing polyacrylamide gel electrophoresis (PAGE). , 2013, Methods in enzymology.
[17] P. Burgers. It’s all about flaps , 2011, Cell cycle.
[18] Roberto F. Delgadillo,et al. Spectroscopic Properties of Fluorescein and Rhodamine Dyes Attached to DNA , 2010, Photochemistry and photobiology.
[19] P. Sung,et al. Cooperativity of Mus81·Mms4 with Rad54 in the Resolution of Recombination and Replication Intermediates*S⃞ , 2009, Journal of Biological Chemistry.
[20] S. West,et al. Identification of Holliday junction resolvases from humans and yeast , 2008, Nature.
[21] Warren A. Kibbe,et al. The issue of amalgams. , 1996, Nucleic Acids Res..
[22] David W. Russell,et al. Isolation of DNA fragments from polyacrylamide gels by the crush and soak method. , 2006, CSH protocols.
[23] S. West,et al. Synthetic junctions as tools to identify and characterize Holliday junction resolvases. , 2006, Methods in enzymology.
[24] J. Yates,et al. Slx1-Slx4 are subunits of a structure-specific endonuclease that maintains ribosomal DNA in fission yeast. , 2003, Molecular biology of the cell.
[25] S. Brill,et al. Slx1-Slx4 is a second structure-specific endonuclease functionally redundant with Sgs1-Top3. , 2003, Genes & development.
[26] S. West,et al. Holliday junction resolution in human cells: two junction endonucleases with distinct substrate specificities , 2002, The EMBO journal.
[27] I. Nazarenko,et al. Effect of primary and secondary structure of oligodeoxyribonucleotides on the fluorescent properties of conjugated dyes. , 2002, Nucleic acids research.
[28] J. Yates,et al. Mus81-Eme1 Are Essential Components of a Holliday Junction Resolvase , 2001, Cell.
[29] M. Kubista,et al. Characterization of fluorescein-oligonucleotide conjugates and measurement of local electrostatic potential. , 1998, Biopolymers.
[30] M. F. White,et al. The structure-selectivity and sequence-preference of the junction-resolving enzyme CCE1 of Saccharomyces cerevisiae. , 1996, Journal of molecular biology.
[31] R. G. Lloyd,et al. Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions. , 1994, The EMBO journal.
[32] H. Shinagawa,et al. Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure. , 1991, The EMBO journal.
[33] R. G. Lloyd,et al. Resolution of Holliday junctions in vitro requires the Escherichia coli ruvC gene product. , 1991, Proceedings of the National Academy of Sciences of the United States of America.
[34] S. West,et al. Genetic recombination in Escherichia coli: Holliday junctions made by RecA protein are resolved by fractionated cell-free extracts. , 1990, Proceedings of the National Academy of Sciences of the United States of America.
[35] L. Symington,et al. Partial purification of an enzyme from Saccharomyces cerevisiae that cleaves Holliday junctions. , 1985, Proceedings of the National Academy of Sciences of the United States of America.
[36] J. Sambrook,et al. Molecular Cloning: A Laboratory Manual , 2001 .