Neotetrazolium staining of three avian Mycoplasma serotypes for microagglutination test.

Mycoplasma meleagridis, M. gallisepticum, and M. synoviae antigens were stained with neotetrazolium chloride for use in the microagglutination test. Only the stained antigens of M. gallisepticum were acceptable in color intensity and test readability.