Photosensitizing activity of hematoporphyrin on Staphylococcus aureus cells.

The photosensitizing action of hematoporphyrin (Hp) on two Staphylococcus aureus strains was investigated to determine if the photoprocess induces in vivo damage in DNA in addition to that occurring at the level of the cytoplasmic membrane. The results obtained demonstrate that the photokilling is dependent on the Hp dose even though the two strains, having a similar Hp-binding capacity, show different levels of photosensitivity. The electrophoretic analysis of cytoplasmic membrane proteins and DNA (chromosomal and plasmidial) suggests that the membrane represents the primary target of the photoprocess, while the DNA, that is damaged both in vivo and in vitro only at relatively long irradiation time, might be a secondary target. Moreover, the photoprocess results in mutagenesis for Salmonella typhimurium tester strains. This information is particularly important in view of the potential use of photodynamic therapy for the treatment of microbial infections.

[1]  G. Jori,et al.  HEMATOPORPHYRIN‐SENSITIZED PHOTOINACTIVATION OF Streptococcus faecalis , 1984, Photochemistry and photobiology.

[2]  J. Piette,et al.  Biological consequences associated with DNA oxidation mediated by singlet oxygen. , 1991, Journal of photochemistry and photobiology. B, Biology.

[3]  Z. Malik,et al.  In vivo effects of porphyrins on bacterial DNA. , 1991, Journal of photochemistry and photobiology. B, Biology.

[4]  E. Reddi,et al.  Factors influencing the haematoporphyrin-sensitized photoinactivation of Candida albicans. , 1989, Journal of general microbiology.

[5]  B. Ames,et al.  Revised methods for the Salmonella mutagenicity test. , 1983, Mutation research.

[6]  H. Abe,et al.  ANALYSIS OF VIRAL DNA, PROTEIN AND ENVELOPE DAMAGE AFTER METHYLENE BLUE, PHTHALOCYANINE DERIVATIVE OR MEROCYANINE 540 PHOTOSENSITIZATION , 1995, Photochemistry and photobiology.

[7]  G. Jori,et al.  Tumour photosensitizers: approaches to enhance the selectivity and efficiency of photodynamic therapy. , 1996, Journal of photochemistry and photobiology. B, Biology.

[8]  M. Vaara,et al.  Polycations as outer membrane-disorganizing agents , 1983, Antimicrobial Agents and Chemotherapy.

[9]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[10]  J. Ghuysen,et al.  Membrane-bound DD-carboxypeptidase and LD-transpeptidase of Streptococcus faecalis ATCC 9790. , 1974, European journal of biochemistry.

[11]  J. V. van Lier,et al.  Photosensitizing activity of water- and lipid-soluble phthalocyanines on Escherichia coli. , 1990, FEMS microbiology letters.

[12]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[13]  Z. Malik,et al.  INACTIVATION OF GRAM‐NEGATIVE BACTERIA BY PHOTOSENSITIZED PORPHYRINS , 1992, Photochemistry and photobiology.

[14]  J. V. van Lier,et al.  Photosensitizing activity of water- and lipid-soluble phthalocyanines on prokaryotic and eukaryotic microbial cells. , 1992, Microbios.

[15]  G. Jori,et al.  The photosensitizing activity of haematoporphyrin on mollicutes. , 1985, Journal of general microbiology.