Influence of Surface‐Imprinted Nanoparticles on Trypsin Activity

Here, the modulation of enzyme activity is presented by protein-imprinted nanoparticles produced using a solid-phase approach. Using trypsin as target, binding of the nanoparticles to the enzyme results in its inhibition or in stabilization, depending on the orientation of the immobilized enzyme used during imprinting.

[1]  Sergey A Piletsky,et al.  Advances in the manufacture of MIP nanoparticles. , 2010, Trends in biotechnology.

[2]  B. Ye,et al.  Different Strategies of Covalent Attachment of Oligonucleotide Probe onto Glass Beads and the Hybridization Properties , 2009, Applied biochemistry and biotechnology.

[3]  Karsten Haupt,et al.  Molecularly imprinted polymers: the next generation. , 2003, Analytical chemistry.

[4]  Hiroyuki Koide,et al.  Recognition, neutralization, and clearance of target peptides in the bloodstream of living mice by molecularly imprinted polymer nanoparticles: a plastic antibody. , 2010, Journal of the American Chemical Society.

[5]  R. A. Mashelkar,et al.  Creating a macromolecular receptor by affinity imprinting , 2001 .

[6]  U. Kolb,et al.  Soluble single-molecule nanogels of controlled structure as a matrix for efficient artificial enzymes. , 2006, Angewandte Chemie.

[7]  Klaus Mosbach,et al.  Drug assay using antibody mimics made by molecular imprinting , 1993, Nature.

[8]  Nicholas A Peppas,et al.  Critical review and perspective of macromolecularly imprinted polymers. , 2012, Acta biomaterialia.

[9]  H. Fritz,et al.  Biochemistry and applications of aprotinin, the kallikrein inhibitor from bovine organs. , 1983, Arzneimittel-Forschung.

[10]  B. Sellergren.  Molecularly imprinted polymers: shaping enzyme inhibitors. , 2010, Nature chemistry.

[11]  Y. Okahata,et al.  Peptide imprinted polymer nanoparticles: a plastic antibody. , 2008, Journal of the American Chemical Society.

[12]  H. Zou,et al.  Size-selective proteolysis on mesoporous silica-based trypsin nanoreactor for low-MW proteome analysis. , 2010, Chemical communications.

[13]  Sergey A. Piletsky,et al.  Solid‐Phase Synthesis of Molecularly Imprinted Polymer Nanoparticles with a Reusable Template–“Plastic Antibodies” , 2013, Advanced functional materials.

[14]  K. Shea,et al.  Selective protein capture by epitope imprinting. , 2006, Angewandte Chemie.

[15]  Karsten Haupt,et al.  Molecularly imprinted microgels as enzyme inhibitors. , 2009, Journal of the American Chemical Society.

[16]  S. Ambrosini,et al.  Solid-phase synthesis of molecularly imprinted nanoparticles for protein recognition. , 2013, Chemical communications.