Comparison of the boundary-lubricating ability of bovine synovial fluid, lubricin, and Healon.

Purified human umbilical hyaluronate and a commercial preparation of rooster comb hyaluronate (Healon) intended for intra-articular viscosupplementation did not demonstrate the same degree of boundary-lubricating ability as bovine synovial fluid or its purified lubricating mucin, lubricin (p < 0.01). Boundary lubrication was measured in vitro in an arthrotripsometer oscillating natural latex against polished glass under a load of 0.35 MPa with an entraining velocity of 0.37 mm/s. The two hyaluronate solutions possessed the same hyaluronate concentration as synovial fluid, but Healon was 4.5 times more viscous. Present practice of viscosupplementation therapy for degenerative joint disease is limited and fails to implicate the important role of synovial mucin. Boundary lubrication provided by synovial mucin, independent of its viscosity, is not replicated by hyaluronate hydrogels.

[1]  T. Yamaguchi,et al.  The effect of additive hyaluronic acid on animal joints with experimentally reduced lubricating ability. , 1994, Journal of biomedical materials research.

[2]  F. C. Linn,et al.  Lubrication of animal joints. I. The arthrotripsometer. , 1967, The Journal of bone and joint surgery. American volume.

[3]  T BITTER,et al.  A modified uronic acid carbazole reaction. , 1962, Analytical biochemistry.

[4]  C. McCutchen.  Lubrication of Joints , 1964 .

[5]  W. Simon,et al.  Water content of equine articular cartilage: effects of enzymatic degradation and "artificial fibrillation". , 1982, Connective tissue research.

[6]  S. L. Lee,et al.  Boundary lubricating ability of synovial fluid in degenerative joint disease. , 1978, Arthritis and rheumatism.

[7]  E. Balazs,et al.  Preliminary clinical assessment of Na-hyaluronate injection into human arthritic joints. , 1974, Pathologie-biologie.

[8]  Aviad Ad,et al.  The molecular weight of therapeutic hyaluronan (sodium hyaluronate): how significant is it? , 1994 .

[9]  F. C. Linn,et al.  Lubrication of animal joints. II. The mechanism. , 1968, Journal of biomechanics.

[10]  I. Reimann,et al.  Demonstration of boundary lubrication by synovial fluid. , 1975, Acta orthopaedica Scandinavica.

[11]  D. Swann,et al.  The isolation and partial characterization of the major glycoprotein (LGP-I) from the articular lubricating fraction from bovine synovial fluid. , 1977, The Biochemical journal.

[12]  S. L. Lee,et al.  A Proposed Model of Boundary Lubrication by Synovial Fluid: Structuring of Boundary Water , 1979 .

[13]  J. Schurz,et al.  Rheology of synovial fluid. , 1987, Biorheology.

[14]  E. Balazs,et al.  Clinical studies of the intraarticular injection of HealonR (sodium hyaluronate) in the treatment of osteoarthritis of human knees , 1981 .

[15]  G. Jay,et al.  Characterization of a bovine synovial fluid lubricating factor. I. Chemical, surface activity and lubricating properties. , 1992, Connective tissue research.

[16]  W. Simon.  Wear properties of articular cartilage in vitro. , 1971, Journal of biomechanics.

[17]  McCutchen Cw.  Why did nature make synovial fluid slimy , 1969 .

[18]  S. L. Lee,et al.  Effect of aqueous cations on friction between rubber and glass , 1983 .

[19]  G. Jay,et al.  Characterization of a bovine synovial fluid lubricating factor. III. The interaction with hyaluronic acid. , 1992, Connective tissue research.

[20]  R W Mann,et al.  Cartilage stresses in the human hip joint. , 1994, Journal of biomechanical engineering.

[21]  D Dowson,et al.  Lubrication of synovial membrane. , 1976, Annals of the rheumatic diseases.

[22]  I. Paul,et al.  Lubrication of synovial membrane. , 1971, Annals of the rheumatic diseases.

[23]  F. C. Linn,et al.  Lubrication of animal joints. 3. The effect of certain chemical alterations of the cartilage and lubricant. , 1968, Arthritis and rheumatism.

[24]  S. L. Lee,et al.  Technical note: an improved McCutchen miniviscometer for connective tissue fluids. , 1978, Connective tissue research.

[25]  C. McCutchen.  The frictional properties of animal joints , 1962 .

[26]  G. Jay,et al.  Silver staining of extensively glycosylated proteins on sodium dodecyl sulfate-polyacrylamide gels: enhancement by carbohydrate-binding dyes. , 1990, Analytical biochemistry.

[27]  K. Tokuyasu,et al.  Effects of the molecular weight of hyaluronic acid and its action mechanisms on experimental joint pain in rats. , 1993, Annals of the rheumatic diseases.

[28]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[29]  Pinals Rs.  Pharmacologic treatment of osteoarthritis , 1992 .