LC / MS , GC / MS screening and in vivo anti-inflammatory activity of Malaysian Moringa oleifera Lam leaf extracts and fractions against carrageenan-induced paw oedema in rats

The anti-inflammatory activity of three extracts of Moringa leaf and the fractions of most active extracts was evaluated. Carrageenan-induced paw oedema in rats was used to evaluate the antiinflammatory activity and to determine the effective dose of M. oleifera leaf extract and its fractions. In addition to that, LC/MS and GC/MS analysis of most active fraction were used to identify the phytoconstituents. 95% ethanol extract, at dose of 250 mg/kg body weight in rat, and its dichloromethane fraction found to be the most active as anti-inflammatory. LC/MS identified 18 compounds and GC/MS identified 8 compounds including flavinoids, phenol glucosides, amino acids and vitamin. A more detailed studies, including in human studies, to identify the phytochemical(s) and to establish the mechanism of action responsible for antiinflammatory activity are highly recommended.

[1]  J. Nečas,et al.  Carrageenan: a review. , 2018 .

[2]  S Kumar,et al.  Anti-Inflammatory Potential of Ethyl Acetate Fraction of Moringa oleifera in Downregulating the NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated Macrophages , 2016, Molecules.

[3]  K. Doriya,et al.  Moringa oleifera: A review on nutritive importance and its medicinal application , 2016 .

[4]  R. M. Kumar,et al.  Moringa oleifera attenuates the release of pro-inflammatory cytokines in lipopolysaccharide stimulated human monocytic cell line , 2015 .

[5]  Sanjay Kumar,et al.  Traditional Indian Medicinal Plants as a potential Anti-inflammatory phytomedicine for Psoriasis control , 2015 .

[6]  A. Spada,et al.  Cultivation, Genetic, Ethnopharmacology, Phytochemistry and Pharmacology of Moringa oleifera Leaves: An Overview , 2015, International journal of molecular sciences.

[7]  Yitao Wang,et al.  Review for carrageenan-based pharmaceutical biomaterials: favourable physical features versus adverse biological effects. , 2015, Carbohydrate polymers.

[8]  L. Norling,et al.  The resolution of inflammation: Principles and challenges. , 2015, Seminars in immunology.

[9]  D. Gilroy,et al.  New insights into the resolution of inflammation. , 2015, Seminars in immunology.

[10]  G. Bruges,et al.  Systemic changes following carrageenan-induced paw inflammation in rats , 2015, Inflammation Research.

[11]  C. Jaramillo,et al.  Phytochemical and nutritional properties of dried leaf powder of Moringa oleifera Lam. from Machala El Oro province of Ecuador , 2014 .

[12]  N. El-Shitany,et al.  Ellagic acid protects against carrageenan-induced acute inflammation through inhibition of nuclear factor kappa B, inducible cyclooxygenase and proinflammatory cytokines and enhancement of interleukin-10 via an antioxidant mechanism. , 2014, International immunopharmacology.

[13]  A. Roy,et al.  An Overview on Phytochemistry and Pharmacological Explorations of Moringa oleifera , 2014 .

[14]  Z. Suleiman,et al.  Design, Formulation and Tableting Properties of Aqueous Leaf Extract of Moringa oleifera , 2014 .

[15]  J. Simon,et al.  Determination of flavonoids by LC/MS and anti-inflammatory activity in Moringa oleifera , 2013 .

[16]  S. Arora,et al.  Preparation and Characterization of Phytosomal-Phospholipid Complex of P. Amarus and its Tablet Formulation , 2013 .

[17]  A. Prasad,et al.  Systematic pharmacognostical, phytochemical and pharmacological review on an ethno medicinal plant, Basella alba L. , 2013 .

[18]  Poonam Sharma,et al.  Anti-Inflammatory Activity of Lactobacillus on Carrageenan-Induced Paw Edema in Male Wistar Rats , 2012, International journal of inflammation.

[19]  S. M. Asdaq,et al.  Immunomodulatory activity of methanolic leaf extract of Moringa oleifera in animals. , 2010, Indian journal of physiology and pharmacology.

[20]  Rjces,et al.  Phytoconstituents and Their Mode of Extractions: An Overview , 2016 .

[21]  P. Mathi,et al.  Analysis of Phytochemicals, Antibacterial and Antioxidant activities ofMoringa oleifera Lam. Leaf extract- an in vitro study , 2014 .

[22]  Hajduk Veljkova.  Definition of Inflammation, Causes of Inflammation and Possible Anti-inflammatory Strategies , 2012 .

[23]  R. Garg,et al.  ANTI - INFLAMMATORY EVALUATION OF LEAF EXTRACT OF MORINGA OLEIFERA , 2012 .

[24]  K. Kumari.  GC-MS determination of bioactive components of Canscora perfoliata Lam . ( Gentianaceae ) Thanga , 2012 .

[25]  Carl Nathan,et al.  Nonresolving Inflammation , 2010, Cell.

[26]  R. E. Renitta,et al.  Isolation, analysis and identification of phytochemicals of antimicrobial activity of Moringa oleifera Lam. , 2009 .