N-TERMINAL AMINO ACID SEQUENCE OF A 28 kDa MAJOR SERUM HIGH DENSITY LIPOPROTEIN OF THE RAINBOW TROUT ONCORHYLIVCHUS MYKISS

As there are few data en primary structures of fish apolipoproteins (apo>, we determined the N-terminal sequence of a 28 kDa majer serum protein of rainbow trout, a possible homologue of apoAI protein in higher vertebrates, Rainbow trout serum was separated by two-dimensional electrophoresis. A spot of the 28 kDa protein (pl 5.45) was cut out and directly applied to a protein sequencer. We succeeded in sequencing 30 amino acids from the N-terminal. The sequence ef the 28 kDa protein shared a high similarity with that of apoAI of Atlantic salmon (Satmo salar), These results showed that the 28 kDa protein is apoAI ef rainbow trout,

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