DEGRADATION OF POLYCYCLIC AROMATIC HYDROCARBONS USING BACTERIAL ISOLATE FROM THE CONTAMINATED SOIL AND WHITE ROT FUNGUS PLEUROTUS OSTREATUS

The use of microbial consortia composed of bacteria and fungi presents higher rates of polycyclic aromatic hydrocarbon (PAH) degradation and is, therefore, essential for the remediation of contaminated soils. A microbial consortium compound comprising Ochrobactrum intermedium and white rot fungus Pleurotus ostreatus was found to be capable of degrading PAHs of crude oil in soil. This research studied the degradation capacity of a consortium composed of O. intermedium and P. ostreatus and the degradation capacity of both the bacteria and the fungus individually. The soil was artificially contaminated using two crude oil concentrations corresponding to 86,000 and 172,000 mg kg. The concentrations of PAHs extracted from the two concentrations of crude oil were 138.16 and 268.03 mg kg, respectively, with respective extraction resins of 78.3 and 73.5%. In total, the biodegradation of 10 PAHs were studied. Fluoranthene, indene[1,2,3-cd]pyrene and benzo[g,h,i]perylene were completely removed by the consortium after 50, 80 and 50 d of incubation, respectively. The rate at which anthracene, pyrene, chrysene and benzo[a]anthracene biodegraded in the presence of the microbial consortium were 96%, 86%, 98% and 98%, respectively, after 110 d of incubation. In this study, higher rates of degradation were obtained with the consortium than when the components were used individually, showing that consortia composed of fungi and bacteria are an efficient technology for degrading xenobiotics, as is the case with PAHs.

[1]  S. Vázquez,et al.  Phenanthrene Biodegradation in Soils Using an Antarctic Bacterial Consortium , 2006 .

[2]  H. Hong,et al.  Analysis of community structure of a microbial consortium capable of degrading benzo(a)pyrene by DGGE. , 2009, Marine pollution bulletin.

[3]  M. T. Moreira,et al.  Biodegradation of polycyclic aromatic hydrocarbons in forest and salt marsh soils by white-rot fungi , 2006 .

[4]  Hauke Harms,et al.  Principles of microbial PAH-degradation in soil. , 2005, Environmental pollution.

[5]  Hauke Harms,et al.  Taking the fungal highway: mobilization of pollutant-degrading bacteria by fungi. , 2005, Environmental science & technology.

[6]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[7]  Jean-François Ponge,et al.  Comparison of a bioremediation process of PAHs in a PAH-contaminated soil at field and laboratory scales. , 2012, Environmental pollution.

[8]  M. Vainstein,et al.  Biodegradation potential of oily sludge by pure and mixed bacterial cultures. , 2011, Bioresource technology.

[9]  Sheng-Shung Cheng,et al.  Bioremediation of petroleum hydrocarbon contaminated soil: Effects of strategies and microbial community shift , 2011 .

[10]  M. Sarrà,et al.  Effect of soil bacteria on the ability of polycyclic aromatic hydrocarbons (PAHs) removal by Trametes versicolor and Irpex lacteus from contaminated soil. , 2010 .

[11]  J. M. Park,et al.  Synergic degradation of phenanthrene by consortia of newly isolated bacterial strains. , 2009, Journal of biotechnology.

[12]  C. Inoue,et al.  Degradation potential and microbial community structure of heavy oil-enriched microbial consortia from mangrove sediments in Okinawa, Japan , 2013, Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering.

[13]  C. Inoue,et al.  Preferential degradation of aromatic hydrocarbons in kerosene by a microbial consortium , 2010 .

[14]  E. Aranda,et al.  Conversion of polycyclic aromatic hydrocarbons, methyl naphthalenes and dibenzofuran by two fungal peroxygenases , 2010, Biodegradation.

[15]  D. Springael,et al.  Microbial community structure of a heavy fuel oil-degrading marine consortium: linking microbial dynamics with polycyclic aromatic hydrocarbon utilization. , 2010, FEMS microbiology ecology.

[16]  L. Cavalca,et al.  Bioremediation of polyaromatic hydrocarbon contaminated soils by native microflora and bioaugmentation with Sphingobium chlorophenolicum strain C3R: A feasibility study in solid- and slurry-phase microcosms , 2011 .

[17]  Y. Hadar,et al.  Initial Oxidation Products in the Metabolism of Pyrene, Anthracene, Fluorene, and Dibenzothiophene by the White Rot Fungus Pleurotus ostreatus , 1996, Applied and environmental microbiology.

[18]  S. Gavazza,et al.  Bioremediation of a tropical clay soil contaminated with diesel oil. , 2012, Journal of environmental management.

[19]  M. García-Martínez,et al.  Biodegradation of Oil Tank Bottom Sludge using Microbial Consortia , 2007, Biodegradation.

[20]  L. F. Bautista,et al.  Effect of surfactants on PAH biodegradation by a bacterial consortium and on the dynamics of the bacterial community during the process. , 2011, Bioresource technology.

[21]  S. Hasnain,et al.  Reduction of toxic hexavalent chromium by Ochrobactrum intermedium strain SDCr-5 stimulated by heavy metals. , 2007, Bioresource technology.

[22]  Jing Wang,et al.  Isolation and characteristics of a microbial consortium for effectively degrading phenanthrene , 2007 .

[23]  M. Petruccioli,et al.  Addition of allochthonous fungi to a historically contaminated soil affects both remediation efficiency and bacterial diversity , 2007, Applied Microbiology and Biotechnology.

[24]  J. González-López,et al.  Selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities. , 2006, Systematic and applied microbiology.

[25]  Mahiran Basri,et al.  Biodegradation of hydrocarbons in soil by microbial consortium , 2004 .

[26]  Removal of Polycyclic Aromatic Hydrocarbons by Pleurotus ostreatus sp . ATCC 38540 in Liquid Medium , 2016 .

[27]  O. Acevedo-Sandoval,et al.  Phylogeny and polycyclic aromatic hydrocarbons degradation potential of bacteria isolated from crude oil-contaminated site , 2017, Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering.

[28]  E. Bååth,et al.  Fungal growth and effects of different wood decomposing fungi on the indigenous bacterial community of polluted and unpolluted soils , 2003, Biology and Fertility of Soils.

[29]  L. F. Bautista,et al.  Isolation and genetic identification of PAH degrading bacteria from a microbial consortium , 2009, Biodegradation.

[30]  K. Kwon,et al.  PAHs contamination and bacterial communities in mangrove surface sediments of the Jiulong River Estuary, China. , 2008, Marine pollution bulletin.

[31]  J. E N N I F E,et al.  Comparative Quantitative Prevalence of Mycobacteria and Functionally Abundant nidA , nahAc , and nagAc Dioxygenase Genes in Coal Tar Contaminated Sediments , 2007 .

[32]  H. Lappin-Scott,et al.  Complications with remediation strategies involving the biodegradation and detoxification of recalcitrant contaminant aromatic hydrocarbons. , 2010, The Science of the total environment.

[33]  X. Santos,et al.  Morphological variability of the Lataste's viper (Vipera latastei) and the Atlas dwarf viper (Vipera monticola): patterns of biogeographical distribution and taxonomy , 2006 .

[34]  L. Boddy,et al.  Saprotrophic basidiomycete mycelia and their interspecific interactions affect the spatial distribution of extracellular enzymes in soil. , 2011, FEMS microbiology ecology.

[35]  E. Favela-Torres,et al.  Design of bacterial defined mixed cultures for biodegradation of specific crude oil fractions, using population dynamics analysis by DGGE ☆ , 2008 .

[36]  J. Mueller,et al.  Action of a Fluoranthene-Utilizing Bacterial Community on Polycyclic Aromatic Hydrocarbon Components of Creosote , 1989, Applied and environmental microbiology.

[37]  J. Hollender,et al.  Assessing the microbial activity of soil samples, its nutrient limitation and toxic effects of contaminants using a simple respiration test. , 2003, Chemosphere.

[38]  K. Jones,et al.  Mycelia promote active transport and spatial dispersion of polycyclic aromatic hydrocarbons. , 2012, Environmental science & technology.

[39]  Xiangui Lin,et al.  Isolation of polycyclic aromatic hydrocarbons (PAHs)-degrading Mycobacterium spp. and the degradation in soil. , 2010, Journal of hazardous materials.

[40]  A. Johnsen,et al.  Evaluation of bacterial strategies to promote the bioavailability of polycyclic aromatic hydrocarbons , 2004, Applied Microbiology and Biotechnology.

[41]  N. Vasudevan,et al.  Role of a moderately halophilic bacterial consortium in the biodegradation of polyaromatic hydrocarbons. , 2009, Marine pollution bulletin.

[42]  A. Hatakka.  Lignin-modifying enzymes from selected white-rot fungi: production and role from in lignin degradation , 1994 .

[43]  Fátima Menezes Bento,et al.  Diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil. , 2005, Microbiology Research.

[44]  E. Chirwa,et al.  Application of biosurfactant produced by Ochrobactrum intermedium CN3 for enhancing petroleum sludge bioremediation , 2015 .

[45]  A. Venosa,et al.  Biodegradation of Crude Oil Contaminating Marine Shorelines and Freshwater Wetlands , 2003 .

[46]  D. Kekos,et al.  Biodegradation of Phenol by Acclimatized Pseudomonas putida Cells Using Glucose as an Added Growth Substrate , 2004, Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering.

[47]  M. Fulekar,et al.  Biodegradation of phenanthrene using adapted microbial consortium isolated from petrochemical contaminated environment. , 2011, Journal of hazardous materials.

[48]  F. Acevedo,et al.  Degradation of polycyclic aromatic hydrocarbons by the Chilean white-rot fungus Anthracophyllum discolor. , 2011, Journal of hazardous materials.

[49]  Jian Mao,et al.  Bioremediation of polycyclic aromatic hydrocarbon-contaminated soil by a bacterial consortium and associated microbial community changes , 2012 .

[50]  Panan Rerngsamran,et al.  Polycyclic aromatic hydrocarbons degradation by Agrocybe sp. CU-43 and its fluorene transformation. , 2009 .

[51]  Mark Tibbett,et al.  Sequential hydrocarbon biodegradation in a soil from arid coastal Australia, treated with oil under laboratory controlled conditions , 2008 .

[52]  Margaret L. Britz,et al.  Degradation and Mineralization of High-Molecular-Weight Polycyclic Aromatic Hydrocarbons by Defined Fungal-Bacterial Cocultures , 2000, Applied and Environmental Microbiology.

[53]  K. Pakshirajan,et al.  Bacterial Degradation of Aromatic Xenobiotic Compounds: An Overview on Metabolic Pathways and Molecular Approaches , 2012 .

[54]  L. F. Bautista,et al.  Effect of different non-ionic surfactants on the biodegradation of PAHs by diverse aerobic bacteria , 2009 .

[55]  J. Borneman,et al.  Molecular microbial diversity of an agricultural soil in Wisconsin , 1996, Applied and environmental microbiology.

[56]  M. Hofrichter,et al.  Degradation of polycyclic aromatic hydrocarbons by manganese peroxidase of Nematoloma frowardii. , 1997, FEMS microbiology letters.

[57]  Qi Li,et al.  Biodegradation of aged polycyclic aromatic hydrocarbons (PAHs) by microbial consortia in soil and slurry phases. , 2008, Journal of hazardous materials.

[58]  P. Baldrian.  Wood-inhabiting ligninolytic basidiomycetes in soils: Ecology and constraints for applicability in bioremediation , 2008 .

[59]  M. Bouchez,et al.  Efficiency of defined strains and of soil consortia in the biodegradation of polycyclic aromatic hydrocarbon (PAH) mixtures , 2004, Biodegradation.

[60]  O. Acevedo-Sandoval,et al.  DEGRADATION OF POLYCYCLIC AROMATIC HYDROCARBONS USING BACTERIAL ISOLATE FROM THE CONTAMINATED SOIL AND WHITE ROT FUNGUS PLEUROTUS OSTREATUS , 2018 .