Monolithically integrated bacteriorhodopsin/semiconductor opto-electronic integrated circuit for a bio-photoreceiver.

The light-sensitive protein, bacteriorhodopsin (BR), is monolithically integrated with an InP-based amplifier circuit to realize a novel opto-electronic integrated circuit (OEIC) which performs as a high-speed photoreceiver. The circuit is realized by epitaxial growth of the field-effect transistors, currently used semiconductor device and circuit fabrication techniques, and selective area BR electro-deposition. The integrated photoreceiver has a responsivity of 175 V/W and linear photoresponse, with a dynamic range of 16 dB, with 594 nm photoexcitation. The dynamics of the photochemical cycle of BR has also been modeled and a proposed equivalent circuit simulates the measured BR photoresponse with good agreement.

[1]  Photo-induced anisotropic photoelectric response in oriented bacteriorhodopsin films , 2003 .

[2]  D. Oesterhelt,et al.  Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane. , 1974, Methods in enzymology.

[3]  H Takei,et al.  Implementing receptive fields with excitatory and inhibitory optoelectrical responses of bacteriorhodopsin films. , 1991, Applied optics.

[4]  J. Czégé,et al.  Restriction of motion of protein side chains during the photocycle of bacteriorhodopsin. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[5]  K. Fukuzawa,et al.  Photoelectrical cell utilizing bacteriorhodopsin on a hole array fabricated by micromachining techniques , 1996 .

[6]  G. Varo,et al.  Kinetic and thermodynamic study of the bacteriorhodopsin photocycle over a wide pH range. , 1998, Biophysical journal.

[7]  A. V. Maximychev,et al.  Oriented purple-membrane films as a probe for studies of the mechanism of bacteriorhodopsin functioning. II. Photoelectric processes , 1987 .

[8]  G. Varo,et al.  The role of water in the extracellular half channel of bacteriorhodopsin. , 1997, Biophysical journal.

[9]  W. Stoeckenius,et al.  Bacteriorhodopsin and the purple membrane of halobacteria. , 1979, Biochimica et biophysica acta.

[10]  G. Varo,et al.  Electric signals during the bacteriorhodopsin photocycle, determined over a wide pH range. , 1998, Biophysical journal.

[11]  Pallab Bhattacharya,et al.  Monolithically integrated bacteriorhodopsin-GaAs field-effect transistor photoreceiver. , 2002, Optics letters.

[12]  H W Trissl,et al.  PHOTOELECTRIC MEASUREMENTS OF MEMBRANES , 1990, Photochemistry and photobiology.

[13]  W. Stoeckenius,et al.  Structure of the purple membrane. , 1971, Nature: New biology.

[14]  R. Birge.  Photophysics and molecular electronic applications of the rhodopsins. , 1990, Annual review of physical chemistry.

[15]  G. Groma,et al.  Study of the photocycle and charge motions of the bacteriorhodopsin mutant D96N. , 1993, Biophysical journal.

[16]  G Büldt,et al.  Reversible loss of crystallinity on photobleaching purple membrane in the presence of hydroxylamine. , 2000, Journal of molecular biology.

[17]  Felix T. Hong,et al.  Molecular sensors based on the photoelectric effect of bacteriorhodopsin: Origin of differential responsivity , 1997 .

[18]  R. Henderson,et al.  Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy. , 1990, Journal of molecular biology.

[19]  V A ́ R O ́ Gy,et al.  Dried oriented purple membrane samples. , 1981 .

[20]  T. Miyasaka,et al.  Quantum Conversion and Image Detection by a Bacteriorhodopsin-Based Artificial Photoreceptor , 1992, Science.

[21]  H. G. Khorana,et al.  Amino acid sequence of bacteriorhodopsin. , 1979, Proceedings of the National Academy of Sciences of the United States of America.