Isotype analysis of the anti-CENP-B anticentromere autoantibody: evidence for restricted clonality.

Utilizing the centromere B fusion protein (CENP-B) and specific, matched monoclonal antiisotype reagents in an enzyme-linked immunosorbent assay, we found that anti-CENP-B autoantibodies were skewed to the IgG1 isotype. The overall kappa:lambda light chain ratio was 2:1, although several individual sera showed a strong predominance of one of the light chains. Isoelectric focusing of light chain-skewed sera showed polyclonal patterns. Our findings are consistent with the anti-CENP-B autoantibody response being a chronic, antigen-driven response.

[1]  K. Elkon,et al.  Immunoblot analysis of IgG subclasses of multiple lupus autoantibodies. , 1988, Journal of immunology.

[2]  W. Earnshaw,et al.  Detection of anticentromere antibodies using cloned autoantigen CENP-B. , 1987, Arthritis and rheumatism.

[3]  R. Eisenberg,et al.  The subclass distribution of human IgG rheumatoid factor. , 1987, Journal of immunology.

[4]  W. Earnshaw,et al.  Analysis of anticentromere autoantibodies using cloned autoantigen CENP-B. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[5]  E. Chan,et al.  IgG subclasses of autoantibodies in systemic lupus erythematosus, Sjogren's syndrome, and drug-induced autoimmunity. , 1986, Journal of immunology.

[6]  W. Earnshaw,et al.  Three human chromosomal autoantigens are recognized by sera from patients with anti-centromere antibodies. , 1986, The Journal of clinical investigation.

[7]  R. Eisenberg,et al.  Subclass restriction and polyclonality of the systemic lupus erythematosus marker antibody anti-Sm. , 1985, The Journal of clinical investigation.

[8]  L. Staudt,et al.  Inter- and intraclonal diversity in the antibody response to influenza hemagglutinin , 1985, The Journal of experimental medicine.

[9]  R. Jefferis,et al.  IgG subclass distribution of autoantibodies to DNA and to nuclear ribonucleoproteins in autoimmune diseases. , 1984, Immunology.

[10]  G. Blobel,et al.  Synthesis and assembly of human small nuclear ribonucleoproteins generated by cell-free translation. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[11]  E. Tan,et al.  Autoantibody to centromere (kinetochore) in scleroderma sera. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[12]  Thomas L. Rothstein,et al.  The role of clonal selection and somatic mutation in autoimmunity , 1987, Nature.

[13]  R. Eisenberg,et al.  Subclass restriction of anti-SS-B (La) autoantibodies. , 1986, Clinical immunology and immunopathology.

[14]  R. Margolis,et al.  Kinetochore components recognized by human autoantibodies are present on mononucleosomes. , 1985, Molecular and cellular biology.