Effects of different weaning times on the stress response and the intestinal microbiota composition of female forest musk deer (Moschus berezovskii) and their fawns

The effects of mother-infant separation (i.e., weaning) on the physiology, psychology and nutrition of mammalian infants have attracted much attention. Forest musk deer (FMD) is a first-class protected species in China and listed endangered in the IUCN Red List. The captive breeding population is not only an important source for restocking of wild resources, but also a necessary way to supply the market with legal musk. So far, there is no scientific basis for the appropriate separation time of FMD females and their infants. Therefore, we used metagenome sequencing and enzyme-linked immunosorbent assays to study changes in the fecal cortisol concentration, as well as the intestinal microbiome composition and function of females and fawns at three different separation times, i.e., after 80 days, 90 days and 100 days. The results showed that the increment of the cortisol concentration in female FMD increased with increasing lactation time. The increment of cortisol concentration in infant FMD was highest in the 80 days weaning group, but there was no significant difference between the 90 days and the 100 days separation time. Based on the annotation results of COG, KEGG and CAZy databases, the abundance of different functions annotated by the intestinal microbiome of mothers and fawns of the 90 days weaning group changed slightly after separation. Based on the above results, the separation of mother and infant FMD is recommended after 90 days, i.e., the separation time that triggered the lowest rate of weaning stress and that supported a relatively stable gastro-intestinal physiology.

[1]  D. Rubenstein,et al.  Characterization of intestinal microbiota and fecal cortisol, T3, and IgA in forest musk deer (Moschus berezovskii) from birth to weaning , 2021, Integrative zoology.

[2]  Qiye Li,et al.  Large-scale ruminant genome sequencing provides insights into their evolution and distinct traits , 2019, Science.

[3]  Zhengshun Wen,et al.  Weaning Stress Perturbs Gut Microbiome and Its Metabolic Profile in Piglets , 2018, Scientific Reports.

[4]  G. Bruschetta,et al.  Effects of partial versus complete separation after weaning on plasma serotonin, tryptophan and pituitary-adrenal pattern of Anglo-Arabian foals , 2017 .

[5]  Lijun La,et al.  Effect of weaning age on cortisol release in piglets. , 2016, Genetics and molecular research : GMR.

[6]  Xiaolong Wang,et al.  Rumen Bacterial Diversity of 80 to 110-Day-Old Goats Using 16S rRNA Sequencing , 2015, PloS one.

[7]  S. Tangphatsornruang,et al.  Comparative Analysis of Microbial Profiles in Cow Rumen Fed with Different Dietary Fiber by Tagged 16S rRNA Gene Pyrosequencing , 2013, Current Microbiology.

[8]  Xiaofeng Luan,et al.  Behavioral Aspects of Captive Alpine Musk Deer during Non-mating Season: Gender Differences and Monthly Patterns , 2011 .

[9]  B. Roe,et al.  Rumen Microbial Population Dynamics during Adaptation to a High-Grain Diet , 2010, Applied and Environmental Microbiology.

[10]  A. Blikslager,et al.  Early weaning stress impairs development of mucosal barrier function in the porcine intestine. , 2010, American journal of physiology. Gastrointestinal and liver physiology.

[11]  G. Perkins,et al.  Preliminary findings of behavioral patterns in captive alpine musk deer (Moschus sifanicus) and prospects for future conservation , 2010, Turkish Journal of Veterinary & Animal Sciences.

[12]  N. Waran,et al.  The effects of weaning on the domestic horse (Equus caballus) , 2008 .

[13]  Chris T. Tromborg,et al.  Sources of stress in captivity , 2007 .

[14]  J. Spears,et al.  The effect of early weaning on feedlot performance and measures of stress in beef calves. , 2005, Journal of animal science.

[15]  E. Douzery,et al.  Molecular and morphological phylogenies of ruminantia and the alternative position of the moschidae. , 2003, Systematic biology.

[16]  J. Lobato,et al.  Effect of gentle handling on the reactivity of zebu crossed calves to humans , 1997 .

[17]  Feng Xiao-la The Effect of Lactation Period on Reproductive Performance of Female Forest Musk Deer , 2015 .

[18]  K. Xu Research progress in forest musk deer , 2014 .

[19]  Hu Zhouqiang,et al.  The Effect of Weaning Ways on Behavior and Growth Performance of Young Forest Musk Deer , 2013 .

[20]  Xu Zhengqiang Population Characteristics and Fawn Survival in Musk Deer( Moshus moschiferus ) , 2003 .

[21]  C. Wemmer Deer: status survey and conservation action plan , 1998 .

[22]  V. D. Thompson Behavioral response of 12 ungulate species in captivity to the presence of humans , 1989 .