Enzyme entrapped polypyrrole modified electrode for flow-injection determination of glucose.

Immobilization of glucose oxidase in electropolymerized polypyrrole film on the surface of a platinum wire electrode, provides a convenient sensor for flow-injection glucose determination. An upper limit of linear response for 100 microliters injected sample volume was estimated as 20 mM, whereas a 500 microliters injected sample volume gave an estimated detection limit of 0.5 mM. A simple electrode preparation procedure allows quick electrode renewal before each series of measurements.

[1]  Y. Osajima,et al.  Simultaneous determination of glucose, fructose, and sucrose in mixtures by amperometric flow injection analysis with immobilized enzyme reactors. , 1988, Analytical chemistry.

[2]  G. Wallace,et al.  Deposition and electrochemical stripping of mercury ions on polypyrrole based modified electrodes , 1988 .

[3]  Jiri Janata,et al.  Electrochemical and ultraviolet−visible spectroelectrochemical investigation of selectivity of potentiometric gas sensors based on polypyrrole , 1987 .

[4]  L. Berlouis,et al.  Recent advances in electrochemical polymerisation for surface coating , 1986 .

[5]  A. Townshend,et al.  Determination of glucose in blood by flow injection analysis and an immobilized glucose oxidase column , 1985 .

[6]  S. Yamaguchi,et al.  Hydrogen ion selective microelectrode prepared by modifying an electrode with polymers , 1987 .

[7]  R. Murray,et al.  CHEMICALLY MODIFIED ELECTRODES , 1977 .

[8]  William R. Heineman,et al.  Polypyrrole electrode as a detector for electroinactive anions by flow injection analysis , 1986 .

[9]  M. Trojanowicz,et al.  Graphite paste-based enzymatic glucose electrode for flow injection analysis , 1988 .

[10]  A. M. Yacynych,et al.  Chemically modified reticulated vitreous carbon electrode with immobilized enzyme as a detector in flow-injection determination of glucose , 1984 .

[11]  S. Feldberg.  Reinterpretation of polypyrrole electrochemistry. Consideration of capacitive currents in redox switching of conducting polymers , 1984 .

[12]  M. Umaña,et al.  Protein-modified electrodes. The glucose oxidase/polypyrrole system , 1986 .

[13]  R. S. Lawton,et al.  Potentiometric response of graphite electrodes coated with modified polymer films , 1984 .

[14]  Christopher R. Lowe,et al.  Enzyme entrapment in electrically conducting polymers. Immobilisation of glucose oxidase in polypyrrole and its application in amperometric glucose sensors , 1986 .

[15]  R. Dams,et al.  Determination of total particulate mercury in air with the coleman mercury analyzer system , 1980 .

[16]  Horacio A. Mottola,et al.  Enzymic substrate determination in closed flow-through systems by sample injection and amperometric monitoring of dissolved oxygen levels , 1978 .