Influence of medium formula and silver nitrate on in vitro plant regeneration of Zinnia cultivars

The effects of culture medium formula and silver nitrate (AgNO3) on plant regeneration were investigated in vitro using shoot tips of three zinnia cultivars namely Zinnia angustifolia cv. Starbright, Z. haageana cv. Percient Carpet and Z. elegans cv. Dreamland. Shoot organogenesis of all cultivars cultured on KS (Kohlenbach and Schmidt, 1975) medium was found superior to FK (Fukuda and Komamine, 1980), MS (Murashige and Skoog, 1962) and MS. Callus formation was found best on MS medium containing 0.1 M TDZ. The addition of 2 mg/l AgNO3 to KS medium resulted in improvement of the regeneration frequency in Zinnia angustifolia cv. Starbright but not in Z. haageana cv. Percient Carpet and Z. elegans cv. Dreamland. In contrast, callus formation was reduced in all three zinnias. These results indicate that the response to AgNO3 was genotype dependent.

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