Intracellular Acid-Base Regulation during Recovery from Locomotor Activity in the Blue Crab (Callinectes sapidus)

Thirty minutes of strenuous muscular exercise in the blue crab resulted in apronounced drop in pH (ΔpH = -0.2 to -0.47 units) and rise in lactate concentration (Δlactate = +4 to +44 mmol·L⁻¹) in hemolymph, light and dark levator, cheliped, and heart muscles. Muscle pHi was restored to control levels within 1 h, whereas restoration of hemolymph pH was not complete until 4 h postexercise. The lactate load in both hemolymph and levator muscles persisted for 4-8 h. Net H⁺ efflux to the environment was elevated sevenfold after exercise and is thought to be responsible for the rapid correction of muscle pHi. The pattern of lactate and H⁺ dynamics in the levator muscles suggests that the bulk of the lactate load was metabolized in situ.

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