Effects of LncRNA-ATB on human peritoneal mesothelial cells phenotypic transition and proliferation induced by high glucose

[Abstract] (cid:542) Objective (cid:542) To explore the effect of long noncoding RNA-ATB (LncRNA-ATB) on phenotypic transition and proliferation of human peritoneal mesothelial cells (HPMCs) induced by high glucose. Methods (cid:542) HPMCs used in experiment were divided into three groups: control group, mannitol group and hypertonic glucose group. HPMCs in control group received no treatment, and in hypertonic glucose group and mannitol group were treated with 50mmol/L D-glucose and isotonic mannitol for 72 hours, respectively. Real-time PCR was employed to detect the mRNA expression of LncRNA-ATB, E-cadherin, (cid:160) -smooth muscle actin ( (cid:160) -SMA), connective tissue growth factor (CTGF), Cyclin D1, cyclin dependent kinase inhibitor 4 (CDK4), protein 27 (p27) and proliferating cell nuclear antigen (PCNA). Western blotting was performed to detect the proteins expression of E-cadherin, (cid:160) -SMA, CTGF, Cyclin D1, CDK4, p27 and PCNA, and flow cytometry was used to test the cell cycle. Lentivirus artifice was used to up- or down-regulate the expression of LncRNA-ATB in untreated HPMCs. Real-time PCR was employed to detect the mRNA expression of E-cadherin, (cid:160) -SMA and CTGF, Western blotting was performed to detect the proteins expression of E-cadherin, (cid:160) -SMA and CTGF, and flow cytometry was used to test the cell cycle. Results (cid:542) It is revealed by Real-time PCR, Western blotting and flow cytometry that the expressions increased of LncRNA-ATB, (cid:160) -SMA, CTGF, Cyclin D1, CDK4 and PCNA induced by hypertonic glucose, and decreased of E-cadherin and p27 ( P <0.05). Up-regulation of LncRNA-ATB promoted HPMCs phenotypic transition and proliferation, while down-regulation alleviated HPMCs phenotypic transition and proliferation. Conclusion (cid:542) Hypertonic glucose may accelerate HPMCs phenotypic transition and proliferation by up-regulating the expression of LncRNA-ATB.

[1]  Q. Qian,et al.  The MicroRNA-199a/214 Cluster Targets E-Cadherin and Claudin-2 and Promotes High Glucose-Induced Peritoneal Fibrosis. , 2017, Journal of the American Society of Nephrology : JASN.

[2]  David W. Johnson,et al.  Changes in the worldwide epidemiology of peritoneal dialysis , 2017, Nature Reviews Nephrology.

[3]  Hanmin Wang,et al.  The role of SRF- miRNA-143-KLF-4 signal pathway in the HPMC phenotypic transition induced via high glucose , 2016 .

[4]  A. Marchetti,et al.  Molecular Mechanisms Underlying Peritoneal EMT and Fibrosis , 2016, Stem cells international.

[5]  Lijie He,et al.  Twist contributes to proliferation and epithelial-to-mesenchymal transition-induced fibrosis by regulating YB-1 in human peritoneal mesothelial cells. , 2015, The American journal of pathology.

[6]  C. Xiao,et al.  Successful treatment of 54 patients with acute renal failure after cardiac surgery , 2015 .

[7]  Z. Dong,et al.  Transition of Mesothelial Cell to Fibroblast in Peritoneal Dialysis: EMT, Stem Cell or Bystander? , 2015, Peritoneal Dialysis International.

[8]  Shuhan Sun,et al.  A long noncoding RNA activated by TGF-β promotes the invasion-metastasis cascade in hepatocellular carcinoma. , 2014, Cancer cell.

[9]  M. Kitagawa,et al.  Cell cycle regulation by long non-coding RNAs , 2013, Cellular and Molecular Life Sciences.

[10]  D. Struijk,et al.  Peritoneal changes in patients on long-term peritoneal dialysis , 2013, Nature Reviews Nephrology.

[11]  K. Gomes,et al.  Inflammation, neoangiogenesis and fibrosis in peritoneal dialysis. , 2013, Clinica chimica acta; international journal of clinical chemistry.

[12]  Hanmin Wang,et al.  Mechanism of transcription factor Twist on promoting peritoneal fibrosis in patients undergoing peritoneal dialysis , 2013 .

[13]  D. Cacchiarelli,et al.  A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA , 2011, Cell.

[14]  O. Devuyst,et al.  The pathophysiology of the peritoneal membrane. , 2010, Journal of the American Society of Nephrology : JASN.

[15]  C. Ponting,et al.  Evolution and Functions of Long Noncoding RNAs , 2009, Cell.

[16]  Yong-Lim Kim.  Update on Mechanisms of Ultrafiltration Failure , 2009, Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis.

[17]  J. Jimenez-Heffernan,et al.  Peritoneal dialysis and epithelial-to-mesenchymal transition of mesothelial cells. , 2003, The New England journal of medicine.

[18]  P. Li,et al.  Current Challenges and Opportunities in PD. , 2017, Seminars in nephrology.

[19]  M. Bajo,et al.  Peritoneal Membrane Preservation. , 2017, Seminars in nephrology.

[20]  Zhang Xiangme.  Expression profiles of long non-coding RNA in HCC patients suffering hepatitis B liver cirrhosis , 2015 .