Early biochemical changes in kerosene exposed rat lungs

Abstract The activities of enzymes, which detoxify oxygen free radical species directly or indirectly were studied in lungs of kerosene-treated rats after 1,4,8 and 16 days of exposure. In the kerosene-exposed animals, activities of glutathione peroxidase (GP), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PD) and glutathione-s-transferase (GST) were significantly increased; thiobarbituric acid (TBA) - reacting products of lipid peroxidation and reduced glutathione (GSH) content were also significantly increased in the lungs. The increase in reduced glutathione content and elevations in the activities of enzymes and products of lipid peroxidation are suggestive of oxidative stress in the lungs of kerosene exposed animals.

[1]  B. Mannervik,et al.  Purification and characterization of the flavoenzyme glutathione reductase from rat liver. , 1975, The Journal of biological chemistry.

[2]  P. McCray,et al.  Effect of glutathione peroxidase activity on lipid peroxidation in biological membranes. , 1976, Biochimica et biophysica acta.

[3]  J. Willerson,et al.  Evaluation of free radical effects and catecholamine alterations in adriamycin cardiotoxicity. , 1984, The American journal of pathology.

[4]  J. Remacle,et al.  Comparative study of the enzymatic defense systems against oxygen-derived free radicals: the key role of glutathione peroxidase. , 1987, Free radical biology & medicine.

[5]  W B Jakoby,et al.  Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. , 1974, The Journal of biological chemistry.

[6]  G. Duggin,et al.  Low activities of glutathione-related enzymes as factors in the genesis of urinary bladder cancer. , 1984, Cancer research.

[7]  J. Depierre,et al.  Preparation and characterization of total, rough and smooth microsomes from the lung of control and methylcholanthrene-treated rats. , 1977, Biochimica et biophysica acta.

[8]  J. Sedlák,et al.  Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. , 1968, Analytical biochemistry.

[9]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[10]  H. W. Gerarde.  Toxicological studies on hydrocarbons. V. Kerosine. , 1959, Toxicology and applied pharmacology.

[11]  C. Dillard,et al.  Glutathione peroxidase system and lysozyme in rats exposed to ozone or nitrogen dioxide , 1974 .

[12]  R. Melmed,et al.  Clinical and Therapeutic Aspects of Kerosene Poisoning: A Series of 200 Cases , 1964, British medical journal.

[13]  K. Tewari,et al.  EXPOSURE AND SOLUBILIZATION OF HEPATIC MITOCHONDRIAL SHUNT DEHYDROGENASES. , 1965, Archives of biochemistry and biophysics.

[14]  J. Crapo,et al.  Tolerance and cross-tolerance using NO2 and O2 II. Pulmonary morphology and morphometry. , 1978, Journal of applied physiology: respiratory, environmental and exercise physiology.

[15]  W. S. Thayer.  Evaluation of tissue indicators of oxidative stress in rats treated chronically with adriamycin. , 1988, Biochemical pharmacology.

[16]  M. Sagai,et al.  Studies on biochemical effects of nitrogen dioxide. III. Changes of the antioxidative protective systems in rat lungs and of lipid peroxidation by chronic exposure. , 1982, Toxicology and applied pharmacology.

[17]  D. Roy,et al.  Changes in activities of free radical detoxifying enzymes in kidneys of male Syrian hamsters treated with estradiol. , 1989, Cancer research.

[18]  S. Rabkin,et al.  Adriamycin cardiomyopathy: implications of cellular changes in a canine model with mild impairment of left ventricular function. , 1985, Biochemical pharmacology.

[19]  Rai Uc,et al.  Cardio-pulmonary changes in mongrel dogs after exposure to kerosene smoke. , 1980 .

[20]  S. Grisolía,et al.  Changes in glutathione in acute and chronic alcohol intoxication , 1980, Pharmacology Biochemistry and Behavior.