Embryos cultured in vitro with multinucleated blastomeres have poor implantation potential in human in-vitro fertilization and intracytoplasmic sperm injection.

The aim of the present study was to investigate pregnancy rates ensuing from transfer of embryos with multinucleated blastomeres. In our in-vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) programme, 1735 embryo transfers were performed from January 1, 1995 to August 31, 1996. In 136 of these transfers at least one embryo with one or more multinucleated blastomeres was present per transfer (study group). For each of these 136 transfers, two matched controls with transfer of exclusively mononucleated embryos were selected (control group). Matching was carried out according to age, method of fertilization (IVF or ICSI), number of transferred embryos and quality score of transferred embryos. In the study group, there were eight transfers of exclusively multinucleated embryos from which one pregnancy ensued and 128 transfers in which multinucleated and mononucleated embryos were transferred together leading to 23 pregnancies. The overall clinical pregnancy rate per transfer was 16.9% in the study group versus 28.7% in the control group (P = 0.01). The ongoing pregnancy rate per transfer was 13.2% in the study group versus 23.2% in the control group (P = 0.03). The implantation rate per transferred embryo was 6.0% in the study group versus 11.3% in the control group (P = 0.003). This study shows that embryos with one or more multinucleated blastomeres have a poorer implantation potential than embryos with mononucleated blastomeres. Transfer of embryos with multinucleated blastomeres should hence only be considered when insufficient numbers of embryos with only mononucleated blastomeres are present.

[1]  A. Trounson,et al.  Evaluation of Normal and Abnormal Human Embryo Development During Procedures In Vitro , 1983 .

[2]  H Balakier,et al.  The frequency and developmental capability of human embryos containing multinucleated blastomeres. , 1997, Human reproduction.

[3]  A. Handyside,et al.  Human preimplantation development in vitro is not adversely affected by biopsy at the 8-cell stage. , 1990, Human reproduction.

[4]  S J Pickering,et al.  The incidence of abnormal morphology and nucleocytoplasmic ratios in 2-, 3- and 5-day human pre-embryos. , 1991, Human reproduction.

[5]  A. T. Sumner,et al.  Post-fertilization polyploidy in human preimplantation embryos fertilized in-vitro. , 1987, Human reproduction.

[6]  S J Pickering,et al.  Transient cooling to room temperature can cause irreversible disruption of the meiotic spindle in the human oocyte. , 1990, Fertility and sterility.

[7]  H. Weier,et al.  Chromosome abnormalities in human arrested preimplantation embryos: a multiple-probe FISH study. , 1994, American journal of human genetics.

[8]  A. De Paepe,et al.  Triple colour fluorescent in-situ hybridization for chromosomes X,Y and 1 on spare human embryos. , 1997, Human reproduction.

[9]  J. Grifo,et al.  Embryo morphology, developmental rates, and maternal age are correlated with chromosome abnormalities. , 1995, Fertility and sterility.

[10]  I. Johnston,et al.  The Fine Structure of Normal and Abnormal Human Embryos Developed in Culture , 1983 .

[11]  S J Pickering,et al.  An analysis of multinucleated blastomere formation in human embryos. , 1995, Human reproduction.

[12]  N. Winston,et al.  Assessment of the cellular DNA content of whole mounted mouse and human oocytes and of blastomeres containing single or multiple nuclei , 1993, Zygote.

[13]  J Tesarík,et al.  Ultrastructural and autoradiographic observations on multinucleated blastomeres of human cleaving embryos obtained by in-vitro fertilization. , 1987, Human reproduction.

[14]  Z. Rosenwaks,et al.  Diagnosis of major chromosome aneuploidies in human preimplantation embryos. , 1993 .

[15]  H. M. Beier,et al.  Fertilization of the Human Egg In Vitro , 1983, Springer Berlin Heidelberg.

[16]  Z. Rosenwaks,et al.  Assessment of numeric abnormalities of X, Y, 18, and 16 chromosomes in preimplantation human embryos before transfer. , 1995, American journal of obstetrics and gynecology.

[17]  J Cohen,et al.  Unsuitability of multinucleated human blastomeres for preimplantation genetic diagnosis. , 1993, Human reproduction.

[18]  M. Alikani,et al.  The presence of multinucleated blastomeres in human embryos is correlated with chromosomal abnormalities. , 1996, Human reproduction.