Secondary structure of acyl carrier protein as derived from two-dimensional 1H NMR spectroscopy.

Sequence-specific assignments of 1H NMR resonances were obtained for the backbone protons in acyl carrier protein (ACP) from Escherichia coli, a protein of 77 residues. The observations, in the NOESY spectra, of 1H-1H sequential and medium-range connectivities indicate the presence of three or four alpha-helical segments joined by short sequences of mixed conformations. The observations are used to refine a secondary structure model previously proposed on the basis of a Chou-Fasman algorithm [Rock, C. O., & Cronan, J. E., Jr. (1979) J. Biol. Chem. 254, 9778-9785].

[1]  K Wüthrich,et al.  Sequential resonance assignments as a basis for determination of spatial protein structures by high resolution proton nuclear magnetic resonance. , 1982, Journal of molecular biology.

[2]  G. Bodenhausen,et al.  Exploring nuclear spin systems by relayed magnetization transfer , 1982 .

[3]  Kurt Wüthrich,et al.  Homonuclear two-dimensional 1H NMR of proteins. Experimental procedures , 1984 .

[4]  D. States,et al.  A two-dimensional nuclear overhauser experiment with pure absorption phase in four quadrants☆ , 1982 .

[5]  Ad Bax,et al.  Investigation of complex networks of spin-spin coupling by two-dimensional NMR , 1981 .

[6]  Kurt Wüthrich,et al.  Systematic application of two-dimensional 1H nuclear-magnetic-resonance techniques for studies of proteins. 2. Combined use of correlated spectroscopy and nuclear Overhauser spectroscopy for sequential assignments of backbone resonances and elucidation of polypeptide secondary structures. , 1981, European journal of biochemistry.

[7]  Richard R. Ernst,et al.  Investigation of exchange processes by two‐dimensional NMR spectroscopy , 1979 .

[8]  K. Wüthrich,et al.  Improved spectral resolution in cosy 1H NMR spectra of proteins via double quantum filtering. , 1983, Biochemical and biophysical research communications.

[9]  G. Wider,et al.  Assignment of the 1H nuclear magnetic resonance spectrum of the proteinase inhibitor IIA from bull seminal plasma by two-dimensional nuclear magnetic resonance at 500 MHz. , 1983, Journal of molecular biology.

[10]  C. Dobson,et al.  Assignment of resonances in the 1H NMR spectrum of human lysozyme. , 1985, European journal of biochemistry.

[11]  D. Wemmer,et al.  Proton NMR studies of .lambda. cro repressor. 2. Sequential resonance assignments of the proton NMR spectrum , 1985 .

[12]  J. Richardson,et al.  Preliminary X-ray diffraction studies of acyl carrier protein from Escherichia coli. , 1985, Journal of molecular biology.

[13]  Kurt Wüthrich,et al.  Secondary structure of the α-amylase polypeptide inhibitor Tendamistat from Streptomyces tendae determined in solution by 1H nuclear magnetic resonance , 1985 .

[14]  Kurt Wüthrich,et al.  Experimental techniques of two-dimensional correlated spectroscopy , 1980 .

[15]  H. Schulz.  On the structure-function relationship of acyl carrier protein of Escherichia coli. , 1975, The Journal of biological chemistry.

[16]  K Wüthrich,et al.  Polypeptide secondary structure determination by nuclear magnetic resonance observation of short proton-proton distances. , 1984, Journal of molecular biology.

[17]  K. Wüthrich,et al.  Amide protein exchange and surface conformation of the basic pancreatic trypsin inhibitor in solution. Studies with two-dimensional nuclear magnetic resonance. , 1982, Journal of molecular biology.

[18]  J. Elovson,et al.  Acyl carrier protein. XI. The specificity of acyl carrier protein synthetase. , 1969, Journal of Biological Chemistry.

[19]  K Wüthrich,et al.  Systematic application of high-resolution, phase-sensitive two-dimensional 1H-NMR techniques for the identification of the amino-acid-proton spin systems in proteins. Rabbit metallothionein-2. , 1985, European journal of biochemistry.

[20]  K Wüthrich,et al.  A two-dimensional nuclear Overhauser enhancement (2D NOE) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromolecules. , 1980, Biochemical and biophysical research communications.

[21]  R. R. Ernst,et al.  Two‐dimensional spectroscopy. Application to nuclear magnetic resonance , 1976 .

[22]  C. Tanford,et al.  Gross conformation of Escherichia coli acyl carrier protein. , 1968, The Journal of biological chemistry.

[23]  K Wüthrich,et al.  Sequential resonance assignments in protein 1H nuclear magnetic resonance spectra. Basic pancreatic trypsin inhibitor. , 1982, Journal of molecular biology.

[24]  K. Wüthrich,et al.  Secondary structure of the lac repressor DNA-binding domain by two-dimensional 1H nuclear magnetic resonance in solution. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[25]  S. Wakil,et al.  The preparation of tryptic, peptic, thermolysin, and cyanogen bromide peptides from the acyl carrier protein of Escherichia coli. , 1968, The Journal of biological chemistry.

[26]  Prescott Dj,et al.  Acyl Carrier Protein , 1972 .

[27]  S. Wakil,et al.  The complete amino acid sequence of the acyl carrier protein of Escherichia coli. , 1968, Journal of Biological Chemistry.

[28]  K Wüthrich,et al.  Sequential individual resonance assignments in the 1H‐NMR spectra of polypeptides and proteins , 1983, Biopolymers.

[29]  J. Prestegard,et al.  Acyl carrier protein from Escherichia coli I. Aspects of the solution structure as evidenced by proton nuclear Overhauser experiments at 500 MHz. , 1983, Biochemistry.

[30]  S. Wakil,et al.  Fatty acid synthesis and its regulation. , 1983, Annual review of biochemistry.

[31]  K Wüthrich,et al.  Sequential resonance assignments in protein 1H nuclear magnetic resonance spectra. Computation of sterically allowed proton-proton distances and statistical analysis of proton-proton distances in single crystal protein conformations. , 1982, Journal of molecular biology.

[32]  A. Bax,et al.  Optimization of two-dimensional homonuclear relayed coherence transfer NMR spectroscopy , 1985 .

[33]  A. Wand,et al.  Two-dimensional 1H NMR studies of cytochrome c. , 1985, Biochemistry.

[34]  C. Rock,et al.  Re-evaluation of the solution structure of acyl carrier protein. , 1979, The Journal of biological chemistry.

[35]  K. Wüthrich,et al.  Sequence-specific resonance assignments in the 1H nuclear-magnetic-resonance spectrum of the lac repressor DNA-binding domain 1-51 from Escherichia coli by two-dimensional spectroscopy. , 1983, European journal of biochemistry.

[36]  P. Y. Chou,et al.  Empirical predictions of protein conformation. , 1978, Annual review of biochemistry.

[37]  C. Rock,et al.  Improved purification of acyl carrier protein. , 1980, Analytical biochemistry.

[38]  K Wüthrich,et al.  Secondary structure in the solution conformation of the proteinase inhibitor IIA from bull seminal plasma by nuclear magnetic resonance. , 1984, Journal of molecular biology.