Bone marrow mesenchymal cells for haemophilia A gene therapy using retroviral vectors with modified long‐terminal repeats

1. Van Damme, A, Chuah, MKL, Dell’accio, F et al. Bone marrow mesenchymal cells for haemophilia A gene therapy using retroviral vectors with modified longterminal repeats. Haemophilia 2003; 9: 94–103. Fig. 2. Differentiation potential of expanded BM mesenchymal cell population. Untransduced BM mesenchymal cells that were kept under normal cell culture conditions (magnification 710 ·) (A). Oil red O staining of cultures induced to differentiate into adipocytes (indicated by arrow) (magnification 710 ·) (B). Calcium deposit staining with alizirin red S solution of undifferentiated (top) and differentiated human BM mesenchymal cells (bottom) after induction of osteogenic differentiation (C). Alkaline phosphatase (ALP) activity normalized over total protein (TP) content of undifferentiated (top) and differentiated human BM mesenchymal cells (bottom) after induction of osteogenic differentiation (D). ALP-specific staining on human BM mesenchymal cells after osteogenic differentiation (magnification 175 ·) (E). Haemophilia (2003), 9, 345