A method for evaluating cold tolerance in rice during reproductive growth stages under natural low-temperature conditions in tropical highlands in Kenya

ABSTRACT Cold tolerance of New Rice for Africa (NERICA) and their parent varieties was evaluated under natural air temperature conditions of Kenyan tropical highlands using the multiple-sown field evaluation method. Rice varieties were cultivated 10 times to expose them to a shift in temperature during 20-day periods before heading (critical period), measuring the average daily mean temperature during the critical period (Tmean) for each variety and filled grain ratio, which was used as an indicator of cold tolerance. Tmean of each variety was low in 6 out of 10 experiments. In the remaining four high-temperature experiments, there were no significant varietal differences in filled grain ratio. In the six low-temperature experiments, all varieties experienced 20.1−21.5°C Tmean. After comparison under virtually the same cold stress, NERICA 1, 3, 4, 7−10, 12, 17, 18, and WAB 56–104 were judged as cold-tolerant; NERICA 2, 5, 11, 13, and 14, and WAB 56–50 and 181–18 were moderately tolerant, and NERICA 6, 15, and 16 were cold-susceptible varieties. These evaluations generally corresponded with previous experimental results under temperature-controlled environments, showing the practicality of multiple-sown field cold tolerance evaluation in Kenya. It also indicated that NERICA varieties, which recorded different levels of cold tolerance and whose average number of days to heading in all experiments were 66−78, are suitable as a set of standard check varieties in this region to evaluate cold tolerance of rice varieties with this range of days to heading. Graphical Abstract

[1]  Mayumi Kikuta,et al.  Analysis of rice yield response to various cropping seasons to develop optimal cropping calendars in Mwea, Kenya , 2020 .

[2]  H. Kitano,et al.  Development of rice breeding and cultivation technology tailored for Kenya's environment , 2018 .

[3]  P. V. Oort.  Mapping abiotic stresses for rice in Africa: Drought, cold, iron toxicity, salinity and sodicity. , 2018 .

[4]  P. V. van Oort,et al.  Mapping abiotic stresses for rice in Africa: Drought, cold, iron toxicity, salinity and sodicity , 2018, Field crops research.

[5]  H. Kitano,et al.  Identification and validation of QTLs for cold tolerance at the booting stage and other agronomic traits in a rice cross of a Japanese tolerant variety, Hananomai, and a NERICA parent, WAB56-104 , 2018 .

[6]  Mayumi Kikuta,et al.  Development of a New Cultivation Technology for Cold Stress Escape through Flowering Time Manipulation by Water Management in the Highlands of East Africa , 2017 .

[7]  P. V. Oort,et al.  Intensification of an irrigated rice system in Senegal: Crop rotations, climate risks, sowing dates and varietal adaptation options , 2016 .

[8]  A. Sow,et al.  Agro-Morphological Evaluation of Rice (Oryza sativa L.) for Seasonal Adaptation in the Sahelian Environment , 2016 .

[9]  H. Kitano,et al.  Evaluation of Cold Tolerance in NERICAs Compared with Japanese Standard Rice Varieties at the Reproductive Stage , 2015 .

[10]  M. Dingkuhn,et al.  Field phenomics for response of a rice diversity panel to ten environments in Senegal and Madagascar. 2. Chilling-induced spikelet sterility , 2015 .

[11]  Xian-Jun Song,et al.  A mathematical model of phloem sucrose transport as a new tool for designing rice panicle structure for high grain yield. , 2015, Plant & cell physiology.

[12]  A. O. Onyango.  Exploring Options for Improving Rice Production to Reduce Hunger and Poverty in Kenya , 2014 .

[13]  R. A. Sperotto,et al.  Avoiding damage and achieving cold tolerance in rice plants , 2013 .

[14]  E. Scopel,et al.  Upland rice production under conservation agriculture cropping systems in cold conditions of tropical highlands , 2012 .

[15]  F. Asch,et al.  Phenological responses of upland rice grown along an altitudinal gradient , 2013 .

[16]  K. Bantte,et al.  Evaluation of Upland NERICA Rice (Oryza sativa L.) Genotypes for Grain Yield and Yield Components along an Altitude Gradient in Southwest Ethiopia , 2011 .

[17]  S. Fukai,et al.  Cold tolerance in rice varieties at different growth stages , 2009 .

[18]  I. Akintayo,et al.  Seed fertility of F1 hybrids between upland rice NERICA cultivars and Oryza sativa L. or O. glaberrima Steud. , 2009 .

[19]  R. L. Williams,et al.  Genotypic variation for cold tolerance during reproductive development in rice : Screening with cold air and cold water , 2006 .

[20]  S. Milach,et al.  Rice cold tolerance at the reproductive stage in a controlled environment , 2006 .

[21]  R. L. Williams,et al.  Minimising cold damage during reproductive development among temperate rice genotypes. II. Genotypic variation and flowering traits related to cold tolerance screening , 2006 .

[22]  M. Dingkuhn,et al.  Interspecific Oryza Sativa L. X O. Glaberrima Steud. progenies in upland rice improvement , 1997, Euphytica.

[23]  P.Monty Jones,et al.  Diversity and Potential of Oryza glaberrima Steud in Upland Rice Breeding , 1997 .

[24]  M. Dingkuhn,et al.  Climatic determinants of irrigated rice performance in the Sahel — I. Photothermal and micro-climatic responses of flowering , 1995 .

[25]  S. Yoshida.  Fundamentals of rice crop science , 1981 .

[26]  T. Satake.  Determination of the most sensitive stage to sterile-type cool injury in rice plants. , 1976 .

[27]  T. Satake.  Research on Cool Injury of Paddy Rice Plants in Japan , 1969 .