The immunologic release of constituents from neutrophil leukocytes. II. Mechanisms of release during phagocytosis, and adherence to nonphagocytosable surfaces.

In contrast, while phagocytosis of particulate complexes stimulated release of materials from the primary granules, alkaline phosphatase was released in only small amounts. A possible mechanism of enzyme release during phagocytosis of large particles was demonstrated to occur by two steps. Degranulation into the phagocytic vacuole was followed by opening of the vacuole during uptake of additional particles. Neutrophils were not lysed during either release process.