Endocytoscopy: novel endoscopic imaging technology for in-situ observation of bladder cancer cells.

BACKGROUND AND PURPOSE Recent advances in endoscopy have enabled microscopic imaging of live bladdercancer cells in situ. The newly developed Endocystoscopy system (Olympus Medical Systems Co., Tokyo, Japan) is a flexible digital endoscope that has more than a 450-fold magnifying power on a video monitor. PATIENTS AND METHODS This supermagnifying endoscope was used to evaluate bladder carcinoma in five patients through the working channel of a rigid cystoscope. RESULTS The cell structure and nuclear morphology were imaged adequately compared with conventional hematoxylin and eosin staining of a biopsy specimen. The tumors were graded correctly in four of five cases. CONCLUSION This novel technology appears to be useful for histologic diagnosis during endoscopic examinations, potentially allowing us to avoid a conventional biopsy.

[1]  Novel endoscopic imaging techniques toward in vivo observation of living cancer cells in the gastrointestinal tract. , 2005, Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association.

[2]  T. Kawano,et al.  Magnifying Endoscopy, Stereoscopic Microscopy, and the Microvascular Architecture of Superficial Esophageal Carcinoma , 2002, Endoscopy.

[3]  K. Chayama,et al.  Magnifying gastroendoscopy for diagnosis of histologic gastritis in the gastric antrum. , 2004, Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver.

[4]  P. Tan,et al.  Macro-microscopic fluorescence of human bladder cancer using hypericin fluorescence cystoscopy and laser confocal microscopy. , 2003, International journal of oncology.

[5]  Haruhiro Inoue,et al.  In vivo observation of living cancer cells in the esophagus, stomach, and colon using catheter-type contact endoscope, "Endo-Cytoscopy system". , 2004, Gastrointestinal endoscopy clinics of North America.

[6]  Yasushi Sano,et al.  Narrow band imaging: a new diagnostic approach to visualize angiogenesis in superficial neoplasia. , 2005, Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association.

[7]  Y. Sano,et al.  Chromoendoscopy using indigo carmine dye spraying with magnifying observation is the most reliable method for differential diagnosis between non-neoplastic and neoplastic colorectal lesions: a prospective study. , 2004, Endoscopy.

[8]  S. Kudo,et al.  Development of Virtual Histology and Virtual Biopsy Using Laser-scanning Confocal Microscopy , 2003, Scandinavian journal of gastroenterology. Supplement.