Application ofaNovelRadioimmunoassay toIdentify Baculovirus Structural Proteins ThatShareInterspecies Antigenic Determinants

NPV(MNPV), single-nucleocapsid NPV (SNPV), andGV virion polypeptides wereshownto havesimilar antigenic determinants andthusbeimmunologically related. The molecular weights ofthevirion polypeptides withcross-reacting antigenic determinants wereidentified. Antisera prepared topurified A.californica andH.zea MNPV polyhedrin (the occlusion bodyprotein fromNPVs)recognized antigenic determinants onallthepolyhedrins andgranulins (occlusion bodyprotein from GVs)thatweretested. Noimmunological relationship wasdetected between A. californica MNPV polyhedrin andanyoftheA.californica MNPV virion structural polypeptides present oneither thevirus isolated fromocclusion bodies orA.californica MNPV extracellular virus frominfected-cell cultures. Baculoviruses, agroupofenveloped doublestranded DNA aniimal viruses inthefamily Baculoviridae (12), areetiologically involved innaturally occurring infections inmanyspecies spanningsevenorders ofinsects. Different baculovirusspecies havebeenclassified into oneofthree subgroups according tomorphological criteria. Subgroup A viruses, thenuclear polyhedrosis viruses (NPVs), havebeenisolated frommore than300insect species andfromcrustaceans. Thevirions inthissubgroup contain either a single nucleocapsid (SNPV) ormanynucleocapsids(MNPV)perenvelope. BothSNPVsand MNPVsformnuclear inclusions late ininfection inwhichmanyvirions areembedded perinclusionbody. Subgroup B viruses, thegranulosis viruses (GVs), havebeenisolated fromabout 50 species oflepidoptera. Theseviruses haveone nucleocapsid perenvelope andonevirion per inclusion body. Subgroup Cviruses, thenonoccluded viruses, arenotcompared inthis study. Nucleotide sequence homology studies and radioimmunoassays (RIAs) aretwoofthemore sensitive methods usedtodemonstrate genetic

[1]  K. Tweeten,et al.  Characterization of an Extremely Basic Protein Derived from Granulosis Virus Nucleocapsids , 1980, Journal of virology.

[2]  J. Vlak,et al.  On the origin of the polyhedral protein of Autographa californica nuclear polyhedrosis virus. Isolation, characterization, and translation of viral messenger RNA. , 1980, Virology.

[3]  M. Summers,et al.  Restriction Map of Rachiplusia ou and Rachiplusia ou-Autographa californica Baculovirus Recombinants , 1980, Journal of virology.

[4]  C. Payne,et al.  Comparison of Three Methods of ELISA for Baculoviruses , 1980 .

[5]  J. Vlak.  The proteins of nonoccluded Autographa californica nuclear polyhedrosis virus produced in an established cell line of Spodoptera frugiperda , 1979 .

[6]  W. J. McCarthy,et al.  Serological relationships among plusiinae baculoviruses , 1979 .

[7]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[8]  G. Stark,et al.  Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[9]  S. Y. Young,et al.  Serological relationships of five nuclear polyhedrosis viruses from lepidopterous species , 1979 .

[10]  J. D. Harper,et al.  Serological comparison of polyhedron protein and virions from four nuclear polyhedrosis viruses of plusiine larvae (Lepidoptera: Noctuidae) , 1979 .

[11]  M. Summers,et al.  Comparative peptide mapping of baculovirus polyhedrins , 1978 .

[12]  M. Summers,et al.  Analysis of baculovirus genomes with restriction endonucleases. , 1978, Virology.

[13]  M. Summers,et al.  Baculovirus structural polypeptides. , 1978, Virology.

[14]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[15]  K. Weber,et al.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. , 1969, The Journal of biological chemistry.