Genome-wide analysis of rice and Arabidopsis identifies two glyoxalase genes that are highly expressed in abiotic stresses

Glyoxalase pathway, ubiquitously found in all organisms from prokaryotes to eukaryotes, consists of glyoxalase I (GLY I) and glyoxalase II (GLY II) enzymes, which detoxify a cytotoxic molecule, methylglyoxal (MG). Increase in MG has been correlated with various diseases in humans and different abiotic stresses in plants. We have previously shown that overproduction of GLY I and/or GLY II enzymes in transgenic plants provide tolerance towards salinity and heavy metal stresses. We have identified nineteen potential GLY I and four GLY II proteins in rice and twenty two GLY I and nine GLY II proteins in Arabidopsis. An analysis of complete set of genes coding for the glyoxalase proteins in these two genomes is presented, including classification and chromosomal distribution. Expression profiling of these genes has been performed in response to multiple abiotic stresses, in different tissues and during various stages of vegetative and reproductive development using publicly available databases (massively parallel signature sequencing and microarray). AtGLYI8, OsGLYI3, and OsGLYI10 expresses constitutively high in seeds while AtGLYI4, AtGLYI7, OsGLYI6, and OsGLYI11 are highly stress inducible. To complement this analyses, qRT-PCR is performed in two contrasting rice genotypes, i.e., IR64 and Pokkali where OsGLYI6 and OsGLYI11 are found to be highly stress inducible.

[1]  M. Ray,et al.  Methylglyoxal levels in plants under salinity stress are dependent on glyoxalase I and glutathione. , 2005, Biochemical and biophysical research communications.

[2]  Hongwei Li,et al.  Molecular cloning and characterization of a novel glyoxalase I gene TaGly I in wheat (Triticum aestivum L.) , 2010, Molecular Biology Reports.

[3]  T. Gauthier,et al.  Respiration of mitochondria isolated from differentiated and undifferentiated HT29 colon cancer cells in the presence of various substrates and ADP generating systems. , 1990, The International journal of biochemistry.

[4]  Paul J Thornalley.  The glyoxalase system: new developments towards functional characterization of a metabolic pathway fundamental to biological life. , 1990, The Biochemical journal.

[5]  Mukesh Jain,et al.  Genome Analysis F-Box Proteins in Rice . Genome-Wide Analysis , Classification , Temporal and Spatial Gene Expression during Panicle and Seed Development , and Regulation by Light and Abiotic Stress 1 [ W ] [ OA ] , 2007 .

[6]  A. Pareek,et al.  Histidine kinase and response regulator genes as they relate to salinity tolerance in rice , 2009, Functional & Integrative Genomics.

[7]  S. Sopory,et al.  Blue light stimulation of cell proliferation and glyoxalase I activity in callus cultures of Amaranthus paniculatus , 1998 .

[8]  C. Paulus,et al.  Physiological and biochemical characterization of glyoxalase I, a general marker for cell proliferation, from a soybean cell suspension , 2004, Planta.

[9]  M. Eliáš,et al.  Phosphatidic acid produced by phospholipase D is required for tobacco pollen tube growth , 2003, Planta.

[10]  D. Botstein,et al.  Cluster analysis and display of genome-wide expression patterns. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[11]  Veena,et al.  Glyoxalase I from Brassica juncea: molecular cloning, regulation and its over-expression confer tolerance in transgenic tobacco under stress. , 1999, The Plant journal : for cell and molecular biology.

[12]  S. Sopory,et al.  Genetic engineering of the glyoxalase pathway in tobacco leads to enhanced salinity tolerance , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[13]  K. Murata,et al.  Molecular cloning of the Pseudomonas putida glyoxalase I gene in Escherichia coli. , 1987, Biochemical and biophysical research communications.

[14]  Sneh L. Singla-Pareek,et al.  Transcriptome map for seedling stage specific salinity stress response indicates a specific set of genes as candidate for saline tolerance in Oryza sativa L. , 2009, Functional & Integrative Genomics.

[15]  I. Sánchez-Aguayo,et al.  Molecular characterization of glyoxalase-I from a higher plant; upregulation by stress , 1995, Plant Molecular Biology.

[16]  M. Kumar,et al.  Characterization and functional validation of glyoxalase II from rice. , 2007, Protein expression and purification.

[17]  S. Lutts,et al.  Changes in plant response to NaCl during development of rice (Oryza sativa L.) varieties differing in salinity resistance , 1995 .

[18]  M. Tester,et al.  Na+ tolerance and Na+ transport in higher plants. , 2003, Annals of botany.

[19]  Ashutosh Kumar Singh,et al.  MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress , 2007, BMC Genomics.

[20]  V. Talesa,et al.  Glyoxalase I and glyoxalase II from Aloe vera: purification, characterization and comparison with animal glyoxalases. , 1990, Biochemistry international.

[21]  Paul J Thornalley.  Dietary AGEs and ALEs and risk to human health by their interaction with the receptor for advanced glycation endproducts (RAGE)--an introduction. , 2007, Molecular nutrition & food research.

[22]  D. V. Vander Jagt,et al.  Methylglyoxal metabolism and diabetic complications: roles of aldose reductase, glyoxalase-I, betaine aldehyde dehydrogenase and 2-oxoaldehyde dehydrogenase. , 2003, Chemico-biological interactions.

[23]  J. Cock,et al.  Laboratory manual for physiological studies of rice , 1971 .

[24]  Stefan R. Henz,et al.  A gene expression map of Arabidopsis thaliana development , 2005, Nature Genetics.

[25]  Ashwani Pareek,et al.  Genome wide expression analysis of CBS domain containing proteins in Arabidopsis thaliana (L.) Heynh and Oryza sativa L. reveals their developmental and stress regulation , 2009, BMC Genomics.

[26]  A. Pareek,et al.  Enhancing salt tolerance in a crop plant by overexpression of glyoxalase II , 2008, Transgenic Research.

[27]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.

[28]  E. Racker.  The mechanism of action of glyoxalase. , 1951, The Journal of biological chemistry.

[29]  Hong Wang,et al.  Gene Expression Profiles during the Initial Phase of Salt Stress in Rice , 2001, Plant Cell.

[30]  S. Sopory,et al.  The glyoxalase system in higher plants: regulation in growth and differentiation. , 1993, Biochemical Society transactions.

[31]  Yuyuan Li,et al.  The structural modification of DNA nucleosides by nonenzymatic glycation: an in vitro study based on the reactions of glyoxal and methylglyoxal with 2′-deoxyguanosine , 2008, Analytical and bioanalytical chemistry.

[32]  M. Kumar,et al.  Whole-Genome Analysis of Oryza sativa Reveals Similar Architecture of Two-Component Signaling Machinery with Arabidopsis1[W] , 2006, Plant Physiology.

[33]  P. Kinnunen,et al.  Rapid Suppression of Mitochondrial Permeability Transition by Methylglyoxal , 2003, Journal of Biological Chemistry.

[34]  E. Bornberg-Bauer,et al.  The AtGenExpress global stress expression data set: protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses. , 2007, The Plant journal : for cell and molecular biology.

[35]  A. Pareek,et al.  Physiological responses among Brassica species under salinity stress show strong correlation with transcript abundance for SOS pathway-related genes. , 2009, Journal of plant physiology.

[36]  A. Basu,et al.  Control of cell proliferation and differentiation by regulating polyamine biosynthesis in cultures of Brassica and its correlation with glyoxalase-I activity , 1988 .

[37]  Hur-Song Chang,et al.  Expression Profile Matrix of Arabidopsis Transcription Factor Genes Suggests Their Putative Functions in Response to Environmental Stresses , 2002, The Plant Cell Online.

[38]  H. Kitano,et al.  Rice plant development: from zygote to spikelet. , 2005, Plant & cell physiology.

[39]  A. Pareek,et al.  Transgenic Tobacco Overexpressing Glyoxalase Pathway Enzymes Grow and Set Viable Seeds in Zinc-Spiked Soils1 , 2005, Plant Physiology.

[40]  K. Murata,et al.  Molecular cloning of the Pseudomonasputida glyoxalase I gene in Escherichiacoli , 1987 .

[41]  Mukesh Jain,et al.  Genome‐wide identification, classification, evolutionary expansion and expression analyses of homeobox genes in rice , 2008, The FEBS journal.

[42]  S. Yadav,et al.  Transgenic tobacco plants overexpressing glyoxalase enzymes resist an increase in methylglyoxal and maintain higher reduced glutathione levels under salinity stress , 2005, FEBS letters.