Solar-simulated skin adaptation and its effect on subsequent UV-induced epidermal DNA damage.

Repeated skin exposure to ultraviolet radiation leads to increased tolerance for erythema. Whether this tolerance is accompanied by a significant protection against epidermal DNA injury has never been thoroughly investigated. In a first set of experiments we irradiated 25 healthy volunteers three times a week for 3 wk using solar-simulating tanning lamps. In addition, all individuals were exposed to a (challenge) dose of three times the initial minimal erythema dose on a small area of skin before the first and after the final exposure. On both occasions, cyclobutane pyrimidine dimers were quantified in biopsies. As expected, repeated ultraviolet exposures resulted in increased epidermal pigmentation and thickness. The ultraviolet sensitivity for erythema decreased on average by 75%. The cyclobutane pyrimidine dimer formation was reduced on average by 60%. In a second set of experiments, with a group of 13 subjects, DNA repair kinetics were assessed. Within a period of 5 d after a single, slightly erythemal dose (1.2 minimal erythema dose), levels of cyclobutane pyrimidine dimer and p53-expressing cells were determined in skin biopsies. Both markers of DNA damage were elevated upon the single ultraviolet exposure and returned to background levels after 3-4 d. This information is important when trying to minimize the risk of DNA damage accumulation after repeated exposures during a tanning course.

[1]  A. Fleischer,et al.  A survey of attitudes, beliefs, and behavior regarding tanning bed use, sunbathing, and sunscreen use. , 1993, Journal of the American Academy of Dermatology.

[2]  R. Anderson,et al.  The optics of human skin. , 1981, The Journal of investigative dermatology.

[3]  D. Lane,et al.  p53, guardian of the genome , 1992, Nature.

[4]  R. Baan,et al.  Detection of photorepair of UV-induced thymine dimers in human epidermis by immunofluorescence microscopy. , 1990, The Journal of investigative dermatology.

[5]  Youn,et al.  A long‐term time course of colorimetric evaluation of ultraviolet light‐induced skin reactions , 1999, Clinical and experimental dermatology.

[6]  Faten Gad,et al.  Mechanisms and implications of the age‐associated decrease in DNA repair capacity , 2000, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[7]  D. Mitchell.  THE RELATIVE CYTOTOXICITY OF(6–4) PHOTOPRODUCTS AND CYCLOBUTANE DIMERS IN MAMMALIAN CELLS * , 1988, Photochemistry and photobiology.

[8]  M. Oren.  p53: the ultimate tumor suppressor gene? , 1992, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[9]  D. Brash,et al.  UV induces p21WAF1/CIP1 protein in keratinocytes without p53. , 1999, The Journal of investigative dermatology.

[10]  C. Potten,et al.  Tanning in Human Skin Types II and III Offers Modest Photoprotection Against Erythema , 1998, Photochemistry and photobiology.

[11]  A. Ziegler,et al.  Sunlight and sunburn in human skin cancer: p53, apoptosis, and tumor promotion. , 1996, The journal of investigative dermatology. Symposium proceedings.

[12]  V. Clarke,et al.  Suntanning and sun protection: a review of the psychological literature. , 1995, Social science & medicine.

[13]  J. Cuzick,et al.  Photoadaptation to ultraviolet (UV) radiation in vivo: photoproducts in epidermal cells following UVB therapy for psoriasis , 2000 .

[14]  H. Beitner,et al.  Sunbed use in relation to phenotype, erythema, sunscreen use and skin diseases. A questionnaire survey among Swedish adolescents , 1996, The British journal of dermatology.

[15]  T. Krieg,et al.  Adaptive antioxidant response of manganese-superoxide dismutase following repetitive UVA irradiation. , 1999, The Journal of investigative dermatology.

[16]  M. Steenwinkel,et al.  Melanin Offers Protection Against Induction of Cyclobutane Pyrimidine Dimers and 6–4 Photoproducts by UVB in Cultured Human Melanocytes¶ , 2001, Photochemistry and photobiology.

[17]  L. Roza,et al.  Assessment of the Effects of Various UV Sources on Inactivation and Photoproduct Induction in Phage T7 Dosimeter , 1998, Photochemistry and photobiology.

[18]  F. Gruijl,et al.  Induction, repair and accumulation of thymine dimers in the skin of UV-B-irradiated hairless mice. , 1991, Carcinogenesis.

[19]  P. Nasca,et al.  A population-based survey of sun lamp and tanning parlor use in New York State, 1990. , 1994, Journal of the American Academy of Dermatology.

[20]  C. Potten,et al.  The in situ repair kinetics of epidermal thymine dimers and 6-4 photoproducts in human skin types I and II. , 1996, The Journal of investigative dermatology.

[21]  R. Baan,et al.  DETECTION OF CYCLOBUTANE THYMINE DIMERS IN DNA OF HUMAN CELLS WITH MONOCLONAL ANTIBODIES RAISED AGAINST A THYMINE DIMER‐ CONTAINING TETRANUCLEOTIDE , 1988, Photochemistry and photobiology.

[22]  P. Strickland.  Photocarcinogenesis by near-ultraviolet (UVA) radiation in Sencar mice. , 1986, The Journal of investigative dermatology.

[23]  Mackie,et al.  Sunbeds in current use in Scotland: a survey of their output and patterns of use , 1998, The British journal of dermatology.

[24]  D. Barker,et al.  Comparison of the responses of human melanocytes with different melanin contents to ultraviolet B irradiation. , 1995, Cancer research.

[25]  G. Kelfkens,et al.  Tumorigenesis by short-wave ultraviolet A: papillomas versus squamous cell carcinomas. , 1991, Carcinogenesis.

[26]  P. Glazer,et al.  Induction of p53 in mouse cells decreases mutagenesis by UV radiation. , 1995, Carcinogenesis.

[27]  D. Mitchell,et al.  Attenuation of DNA Damage in the Dermis and Epidermis of the Albino Hairless Mouse by Chronic Exposure to Ultraviolet-A and -B Radiation¶ , 2001, Photochemistry and photobiology.

[28]  G. Li,et al.  p53‐Dependent DNA repair and apoptosis respond differently to high‐ and low‐dose ultraviolet radiation , 1998, The British journal of dermatology.

[29]  H. Wulf,et al.  Differences in narrow-band ultraviolet B and broad-spectrum ultraviolet photocarcinogenesis in lightly pigmented hairless mice. , 1994, Photodermatology, photoimmunology & photomedicine.

[30]  H Slaper,et al.  Wavelength dependence of skin cancer induction by ultraviolet irradiation of albino hairless mice. , 1993, Cancer research.

[31]  B. Sutherland,et al.  Comparative protection efficiency of UVA- and UVB-induced tans against erythema and formation of endonuclease-sensitive sites in DNA by UVB in human skin. , 1985, The Journal of investigative dermatology.

[32]  R. Baan,et al.  Removal of UV-induced DNA lesions in mouse epidermis soon after irradiation. , 1994, Journal of photochemistry and photobiology. B, Biology.

[33]  J. Cuzick,et al.  Photoadaptation to ultraviolet (UV) radiation in vivo: photoproducts in epidermal cells following UVB therapy for psoriasis. , 2000, The British journal of dermatology.

[34]  H. Friedman,et al.  Psychosocial factors in sunbathing and sunscreen use. , 1987, Health psychology : official journal of the Division of Health Psychology, American Psychological Association.