Continuous butanol fermentation from xylose with high cell density by cell recycling system.

A continuous butanol production system with high-density Clostridium saccharoperbutylacetonicum N1-4 generated by cell recycling was established to examine the characteristics of butanol fermentation from xylose. In continuous culture without cell recycling, cell washout was avoided by maintaining pH>5.6 at a dilution rate of 0.26 h(-1), indicating pH control was critical to this experiment. Subsequently, continuous culture with cell recycling increased cell concentration to 17.4 g L(-1), which increased butanol productivity to 1.20 g L(-1) h(-1) at a dilution rate of 0.26 h(-1) from 0.529 g L(-1) h(-1) without cell recycling. The effect of dilution rates on butanol production was also investigated in continuous culture with cell recycling. Maximum butanol productivity (3.32 g L(-1) h(-1)) was observed at a dilution rate of 0.78 h(-1), approximately 6-fold higher than observed in continuous culture without cell recycling (0.529 g L(-1) h(-1)).

[1]  N. Qureshi,et al.  Continuous production of acetone-butanol-ethanol using immobilized cells of Clostridium acetobutylicum and integration with product removal by liquid-liquid extraction , 1995 .

[2]  Yukihiro Tashiro,et al.  Efficient conversion of lactic acid to butanol with pH-stat continuous lactic acid and glucose feeding method by Clostridium saccharoperbutylacetonicum , 2010, Applied Microbiology and Biotechnology.

[3]  Yujiu Ma,et al.  Continuous acetone–butanol–ethanol production by corn stalk immobilized cells , 2009, Journal of Industrial Microbiology & Biotechnology.

[4]  A. Afschar,et al.  Production of acetone and butanol from starch by continuous bioprocess , 1991 .

[5]  E. Green.  Fermentative production of butanol--the industrial perspective. , 2011, Current opinion in biotechnology.

[6]  J. M. Engasser,et al.  Continuous acetone-butanol fermentation with high productivity by cell ultrafiltration and recycling , 1986, Biotechnology Letters.

[7]  Thaddeus Chukwuemeka Ezeji,et al.  Butanol, ‘a superior biofuel’ production from agricultural residues (renewable biomass): recent progress in technology , 2008 .

[8]  B. Cheirsilp,et al.  Potential use of Bacillus subtilis in a co-culture with Clostridium butylicum for acetone-butanol-ethanol production from cassava starch. , 2010 .

[9]  G. Goma,et al.  Acetonobutylic fermentation: Improvement of performances by coupling continuous fermentation and ultrafiltration , 1986, Biotechnology and bioengineering.

[10]  Masahiro Okamoto,et al.  Kinetic study of substrate dependency for higher butanol production in acetone–butanol–ethanol fermentation , 2008 .

[11]  Kalyan Gayen,et al.  Developments in biobutanol production: New insights , 2011 .

[12]  Yukihiro Tashiro,et al.  High butanol production by Clostridium saccharoperbutylacetonicum N1-4 in fed-batch culture with pH-Stat continuous butyric acid and glucose feeding method. , 2004, Journal of bioscience and bioengineering.

[13]  Ludo Diels,et al.  Integrated bioprocess for long-term continuous cultivation of Clostridium acetobutylicum coupled to pervaporation with PDMS composite membranes. , 2012, Bioresource technology.

[14]  N. Qureshi,et al.  Continuous solvent production by Clostridium beijerinckii BA101 immobilized by adsorption onto brick , 2000 .

[15]  Karl Schügerl,et al.  Production of acetone and butanol by Clostridium acetobutylicum in continuous culture with cell recycle , 1985, Applied Microbiology and Biotechnology.

[16]  A. Heiningen,et al.  Continuous bio-catalytic conversion of sugar mixture to acetone–butanol–ethanol by immobilized Clostridium acetobutylicum DSM 792 , 2011, Applied Microbiology and Biotechnology.

[17]  R. Toledo,et al.  Continuous acetone–ethanol–butanol fermentation by immobilized cells of Clostridium acetobutylicum , 2001 .

[18]  K. Furukawa,et al.  Production of acetone, butanol and ethanol from palm oil waste by Clostridium saccharoperbutylacetonicum N1-4 , 1995, Biotechnology Letters.

[19]  I. Maddox,et al.  Production of solvents (ABE fermentation) from whey permeate by continuous fermentation in a membrane bioreactor , 1989 .

[20]  K. Okamoto,et al.  Genetic Engineering of Zymobacter palmae for Production of Ethanol from Xylose , 2007, Applied and Environmental Microbiology.

[21]  Y. Tashiro,et al.  Development of high-speed and highly efficient butanol production systems from butyric acid with high density of living cells of Clostridium saccharoperbutylacetonicum. , 2012, Journal of biotechnology.

[22]  T. Ezeji,et al.  Continuous butanol fermentation and feed starch retrogradation: butanol fermentation sustainability using Clostridium beijerinckii BA101. , 2005, Journal of biotechnology.

[23]  Y. Jang,et al.  Butanol production from renewable biomass by clostridia. , 2012, Bioresource technology.

[24]  J. Andrade,et al.  Continuous cultures of Clostridium acetobutylicum: culture stability and low-grade glycerol utilisation , 2004, Biotechnology Letters.

[25]  T. Ezeji,et al.  Production of acetone-butanol-ethanol (ABE) in a continuous flow bioreactor using degermed corn and Clostridium beijerinckii , 2007 .

[26]  A. Heiningen,et al.  Continuous production of isopropanol and butanol using Clostridium beijerinckii DSM 6423 , 2011, Applied Microbiology and Biotechnology.

[27]  Hong-Wei Yen,et al.  The effects of dilution rate and glucose concentration on continuous acetone–butanol–ethanol fermentation by Clostridium acetobutylicum immobilized on bricks , 2011 .

[28]  Yukihiro Tashiro,et al.  High production of acetone-butanol-ethanol with high cell density culture by cell-recycling and bleeding. , 2005, Journal of biotechnology.