BACKGROUND
Platelet transfusion is accompanied by febrile nonhemolytic transfusion reactions. The generation of cytokines (like IL-1 beta, IL-6, IL-8, and TNF-alpha) in platelet concentrates by white cells is suggested to be responsible for febrile nonhemolytic transfusion reactions. The number of white cells in the platelet concentrates is crucial to cytokine generation.
METHODS
This study was performed to determine whether WBC reduction in platelet concentrates by prestorage leukodepletion filters or inactivation by gamma radiation reduced the levels of these cytokines during storage for 3 days. Each of the platelet concentrates (n = 54) was prepared from a single random donor by platelet-rich plasma. This was then divided into four groups: 1) unfiltered, nonirradiated random-donner platelet concentrates (n = 13); 2) unfiltered, gamma-irradiated random-donner platelet concentrates (n = 16); 3) filtered, nonirradiated random-donner platelet concentrates (n = 14); and 4) filtered, gamma-irradiated random-donner platelet concentrates (n = 11). Cytokine levels in platelet concentrates supernatants were measured by ELISA kits according to the manufacturer's recommendations.
RESULTS
Our results showed that IL-8 was detected in unfiltered, nonirradiated, and gamma-irradiated random-donner platelet concentrates but not in the filtered random-donner platelet concentrates. TNF-alpha was only detected in unfiltered, nonirradiated units. Compared with unfiltered platelet concentrates, prestorage filtration prevented a rise in the IL-8 and TNF-alpha on day 3 of storage. The concentration of IL-1 beta was lower than the minimum concentration value of the kit used for this purpose. IL-6 was detected only in 7 units of all filtered platelet concentrates on day 3.
CONCLUSION
These data indicate that gamma irradiation can not prevent generation of IL-8 in platelet concentrates during storage, but prestorage leukoreduction of platelet concentrates can prevent accumulation of IL-6, IL-8, and TNF-alpha during storage.
[1]
J. Bengtson,et al.
Complement activation in prestorage leucocyte‐filtered plasma
,
2004,
Transfusion medicine.
[2]
J. Kelton,et al.
Platelet transfusions in children: results of a randomized, prospective, crossover trial of plasma removal and a prospective audit of WBC reduction
,
2002,
Transfusion.
[3]
K. Thorpe,et al.
A randomized controlled trial comparing the frequency of acute reactions to plasma‐removed platelets and prestorage WBC‐reduced platelets
,
2002,
Transfusion.
[4]
C. Tzeng,et al.
Influence of gamma irradiation and storage on apheresis platelets.
,
2001,
Journal of the Formosan Medical Association = Taiwan yi zhi.
[5]
D. Musiani,et al.
White cell apoptosis in platelet concentrates
,
2000,
Transfusion.
[6]
P. Roux‐Lombard,et al.
Benefit of Prestorage Leukocyte Depletion of Single–Donor Platelet Concentrates
,
1999,
Vox Sanguinis.
[7]
P. Roux‐Lombard,et al.
Benefit of Prestorage Leukocyte Depletion of Single‐Donor Platelet Concentrates
,
1999,
Vox sanguinis.
[8]
N. Grunnet,et al.
Comparison of the level of cytokine mRNA in buffy coat‐derived platelet concentrates prepared with or without white cell reduction by filtration
,
1998,
Transfusion.
[9]
M. Fujihara,et al.
Generation of interleukin 8 in stored apheresis platelet concentrates and the preventive effect of prestorage ultraviolet B radiation
,
1997,
Transfusion.
[10]
A. Giulivi,et al.
Effect of filtration of platelet concentrates on the accumulation of cytokines and platelet release factors during storage
,
1995,
Transfusion.