Phenotypic and molecular characterization of isolates of Ornithobacterium rhinotracheale from Peru.

Twenty-five isolates of Ornithobacterium rhinotracheale were examined by agar gel precipitation, immunoperoxidase assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western blot analysis, and a polymerase chain reaction. All of the isolates were identified as serotype A. Protein profiles of whole cell extracts were similar for all the isolates, and a polypeptide with a molecular weight of 33 kD was a major component, being present in all the isolates. In the main, proteins of 33, 42, 52, and 66 kD were recognized in immunoblots with sera from chickens naturally exposed to O. rhinotracheale. A modified polymerase chain reaction assay identified O. rhinotracheale DNA from all the isolates and tracheal swabs, producing amplicons of 784 bp, and distinguished O. rhinotracheale from bacterial agents causing similar clinical signs.

[1]  H. Hafez,et al.  Ornithobacterium rhinotracheale: A review. , 1999, Avian pathology : journal of the W.V.P.A.

[2]  H. Hafez,et al.  MODERN TECHNIQUES IN DIAGNOSIS OF POULTRY DISEASES : REVIEW , 1999 .

[3]  H. Hafez,et al.  Investigations on different Ornithobacterium rhinotracheale "ORT" isolates. , 1999, Avian diseases.

[4]  H. van den Bosch,et al.  Identification and serotyping of Ornithobacterium rhinotracheale , 1997, Journal of clinical microbiology.

[5]  H. Shivaprasad,et al.  Ornithobacterium rhinotracheale infection in turkey breeders. , 1996, Avian diseases.

[6]  D. Goovaerts,et al.  Experimental infection in turkeys and chickens with Ornithobacterium rhinotracheale. , 1996, Avian diseases.

[7]  A. Travers.  Concomitant Ornithobacterium rhinotracheale and Newcastle disease infection in broilers in South Africa. , 1996, Avian diseases.

[8]  M. Ryll,et al.  Acute exudative pneumonia and airsacculitis associated with Ornithobacterium rhinotracheale in turkeys , 1994, Veterinary Record.

[9]  P. Vandamme,et al.  Ornithobacterium rhinotracheale gen. nov., sp. nov., isolated from the avian respiratory tract. , 1994, International journal of systematic bacteriology.

[10]  C. Cardona,et al.  Preliminary Characterization of a Pleomorphic Gram-Negative Rod Associated with Avian Respiratory Disease , 1993, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[11]  P. Blackall,et al.  Whole-cell protein profiles of Haemophilus paragallinarum as detected by polyacrylamide gel electrophoresis. , 1989, Avian diseases.

[12]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.