Determination of Physostigmine in Plasma by Liquid Chromatography with Dual Electrode Amperometric Detection

Abstract Physostigmine in plasma is determined after solid phase extraction. Ion-pair liquid chromatography is used for the separation and dual electrodes in a series mode are used for the detection. Physostigmine is first oxidized at +1.0 V and the reaction product formed is determined by reduction at +0.10 V versus Ag/AgCl. The solid phase extraction gives a recovery of 62 ± 6% (n=5) from plasma spiked with physostigmine to a concentration of 14 ng/mL. A detection limit of 0.5 ng/mL is obtained from a sample of 2 mL plasma.

[1]  E. Grushka,et al.  System peaks in liquid chromatography: their origin, formation, and importance , 1986 .

[2]  E. Kohlstaedt,et al.  Physostigmine effects in Alzheimer's disease: relationship to dementia severity. , 1986, Life sciences.

[3]  P. Hartvig,et al.  Pharmacokinetics of physostigmine after intravenous, intramuscular and subcutaneous administration in surgical patients , 1986, Acta anaesthesiologica Scandinavica.

[4]  A. Khalique,et al.  Determination of physostigmine in plasma and brain by HPLC. , 1985, Journal of analytical toxicology.

[5]  R. Weinberger,et al.  Selectivity in liquid chromatography with micellar mobile phases , 1982 .

[6]  W. C. Purdy,et al.  Electrochemical detectors in liquid chromatography. A short review of detector design , 1981 .

[7]  A. Porsius,et al.  Quantitative determination of the cholinesterase inhibitor physostigmine in brain tissue samples using reversed-phase high-performance liquid chromatography. , 1981, Journal of chromatography.

[8]  M. Daunderer.  Physostigmine salicylate as an antidote. , 1980, International journal of clinical pharmacology, therapy, and toxicology.

[9]  W. C. Purdy,et al.  The behaviour of an electrochemical detector used in liquid chromatography and continuous flow voltammetry: Part 2. Evaluation of low-temperature isotropic carbon for use as an electrode material , 1978 .

[10]  W. Groff,et al.  Quantitative assay of physostigmine in human whole blood. , 1977, Journal of pharmaceutical sciences.

[11]  B. Patterson,et al.  Colorimetric determination of physostigmine in pharmaceuticals. , 1971, Journal of Pharmacy and Science.

[12]  S. Borst,et al.  Quantitative determination of physostigmine from aqueous solution , 1969, The Journal of pharmacy and pharmacology.

[13]  I. Christenson.  The kinetics of hydrolysis of physostigmine salicylate. , 1969, Acta pharmaceutica Suecica.

[14]  P. Kissinger,et al.  Metabolism of physostigmine in mouse liver microsomal incubations studied by liquid chromatography with dual-electrode amperometric detection. , 1987, Journal of Chromatography A.

[15]  R. Whelpton,et al.  Sensitive liquid chromatographic method for physostigmine in biological fluids using dual-electrode electrochemical detection. , 1985, Journal of chromatography.

[16]  R. Whelpton.  Analysis of plasma physostigmine concentrations by liquid chromatography. , 1983, Journal of chromatography.

[17]  K. Davis,et al.  Rapid High-Performance Liquid Chromatographic Determination of Physostigmine in Plasma , 1982 .

[18]  A. R. Rogers,et al.  The determination of physostigmine by thin-layer chromatography and ultraviolet spectrophotometry. , 1973, Journal of chromatography.

[19]  F. H. Rodin.  Eserine; its history in the practice of ophthalmology (physostigmine; Physostigma venenosum (Balfour); Calabar bean). , 1947, American journal of ophthalmology-glaucoma.

[20]  A. Salway.  CI.—Researches on the constitution of physostigmine. Part I , 1912 .