Analysis of the formation of parallel arrays by BHK cells in vitro.

Abstract The contact behavior of BHK cells in tissue culture was studied in order to elucidate the mechanism for their formation of parallel arrays. It was found that when two arrays of cells meet at angles of less than 55 °, they merge and form a single array. At angles of contact greater than 55 °, these arrays crisscross. This same behavior was noted when two individual cells contacted each other. At angles up to 55 ° the contacting cell alters its direction of movement so that it comes to lie parallel to the contacted cell. At angles greater than 55 °, in contrast, crisscrossing occurs. Detailed analysis at high magnification showed that this behavior is dependent upon localized contact inhibition of cell movement.

[1]  M. Abercrombie,et al.  The locomotion of fibroblasts in culture. I. Movements of the leading edge. , 1970, Experimental cell research.

[2]  I. Macpherson,et al.  Syrian Hamster Fibroblast Cell Line BHK21 and its Derivatives , 1964, Nature.

[3]  T. F. Anderson,et al.  TECHNIQUES FOR THE PRESERVAATION OF THREE-DIMENSIONAL STRUCTURE IN PREPARING SPECIMENS FOR THE ELECTRON MICROSCOPE† , 1951 .

[4]  C. C. Speidel Studies of living nerves. II. Activities of ameboid growth cones, sheath cells, and myelin segments, as revealed by prolonged observation of individual nerve fibers in frog tadpoles , 1933 .

[5]  R. Dehaan,et al.  Migration patterns of the precardiac mesoderm in the early chick embrvo. , 1963, Experimental cell research.

[6]  T. Elsdale Parallel orientation of fibroblasts in vitro. , 1968, Experimental cell research.

[7]  M. Abercrombie,et al.  Adhesions of fibroblasts to substratum during contact inhibition observed by interference reflection microscopy. , 1975, Experimental cell research.

[8]  J. A. Weston,et al.  A radioautographic analysis of the migration and localization of trunk neural crest cells in the chick. , 1963, Developmental biology.

[9]  E. K. Hall Experimental modifications of muscle development in amblystoma punctatum , 1950 .

[10]  L. Hayflick,et al.  The serial cultivation of human diploid cell strains. , 1961, Experimental cell research.

[11]  G. Nicolson,et al.  The cell surface in animal embryogenesis and development , 1979 .

[12]  C. C. Speidel Studies of living nerves. I. The movements of individual sheath cells and nerve sprouts correlated with the process of myelin‐sheath formation in amphibian larvae , 1932 .

[13]  P. Weiss In vitro experiments on the factors determining the course of the outgrowing nerve fiber , 1934 .

[14]  A. Dipasquale Locomotion of epithelial cells. Factors involved in extension of the leading edge. , 1975, Experimental cell research.

[15]  M. Abercrombie,et al.  Invasive behavior between sarcoma and fibroblast populations in cell culture. , 1976, Journal of the National Cancer Institute.

[16]  J. Revel,et al.  Fine structure of the developing avian cornea. , 1969, Monographs in developmental biology.

[17]  J. Trinkaus,et al.  Local inhibition of ruffling during contact inhibition of cell movement. , 1971, Experimental cell research.

[18]  M. Abercrombie,et al.  Observations on the social behaviour of cells in tissue culture. II. Monolayering of fibroblasts. , 1954, Experimental cell research.

[19]  J. Bard,et al.  Cellular Interactions in Mass Cultures of Human Diploid Fibroblasts , 1972, Nature.

[20]  L. Weiss The adhesion of cells. , 1960, International review of cytology.

[21]  Kathryn W. Tosney The early migration of neural crest cells in the trunk region of the avian embryo: an electron microscopic study. , 1978, Developmental biology.