Construction and characterization of a YAC library with a low frequency of chimeric clones from flow-sorted human chromosome 9.

Human chromosome 9 DNA, flow-sorted from somatic cell hybrid PK-87-9, has been used to construct two complete digest YAC libraries. The combined representation of chromosome 9 in these libraries, estimated by hybridization of chromosome 9-specific sequences to YAC colony grids, is approximately 95%. The frequency of chimeric clones, analyzed by fluorescence in situ hybridization of chromosome 9 YACs to human metaphase chromosomes, was estimated to be approximately 4%. These libraries provide a resource for physical mapping and for moving from genetic markers to disease loci on chromosome 9.

[1]  R. Sikorski,et al.  A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. , 1989, Genetics.

[2]  C. L. Jackson,et al.  Generation and characterization of a human chromosome 9 cosmid library , 1992, Somatic cell and molecular genetics.

[3]  David R. Wolf,et al.  Base compositional structure of genomes. , 1992, Genomics.

[4]  C. E. Hildebrand,et al.  Construction of human chromosome-specific DNA libraries from flow-sorted chromosomes. , 1986, Cold Spring Harbor symposia on quantitative biology.

[5]  P. Sharp,et al.  Exon amplification: a strategy to isolate mammalian genes based on RNA splicing. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[6]  S. Antonarakis,et al.  Yeast artificial chromosome vectors for efficient clone manipulation and mapping. , 1991, Genomics.

[7]  S. Antonarakis,et al.  Construction of human chromosome 21-specific yeast artificial chromosomes. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[8]  N. Carter,et al.  Physical mapping within the tuberous sclerosis linkage group in region 9q32-q34. , 1993, Genomics.

[9]  J. Haines,et al.  A genetic linkage map of human chromosome 9q. , 1992, Genomics.

[10]  Ronald W. Davis,et al.  Mitotic stability of yeast chromosomes: A colony color assay that measures nondisjunction and chromosome loss , 1985, Cell.

[11]  J. Haines,et al.  Localization of one gene for tuberous sclerosis within 9q32-9q34, and further evidence for heterogeneity. , 1991, American journal of human genetics.

[12]  M. Ross,et al.  Selection of a human chromosome 21 enriched YAC sub–library using a chromosome–specific composite probe , 1992, Nature Genetics.

[13]  D. Paslier,et al.  Isolation of chromosome 21–specific yeast artificial chromosomes from a total human genome library , 1992, Nature Genetics.

[14]  E. Barillot,et al.  Theoretical analysis of library screening using a N-dimensional pooling strategy. , 1991, Nucleic acids research.

[15]  L. Deaven,et al.  Low-frequency chimeric yeast artificial chromosome libraries from flow-sorted human chromosomes 16 and 21. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[16]  D. Church,et al.  Identification of human chromosome 9 specific genes using exon amplification. , 1993, Human molecular genetics.

[17]  J. Sampson,et al.  Linkage investigation of three putative tuberous sclerosis determining loci on chromosomes 9q, 11q, and 12q. The Tuberous Sclerosis Collaborative Group. , 1992, Journal of medical genetics.

[18]  P. Burgers,et al.  Transformation of yeast spheroplasts without cell fusion. , 1987, Analytical biochemistry.

[19]  M. Olson,et al.  Cloning human telomeric DNA fragments into Saccharomyces cerevisiae using a yeast-artificial-chromosome vector. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[20]  S. Colowick,et al.  Methods in Enzymology , Vol , 1966 .

[21]  C. L. Jackson,et al.  Monochromosomal rodent-human hybrids from microcell fusion of human lymphoblastoid cells containing an inserted dominant selectable marker. , 1990, Genomics.

[22]  J. Gusella,et al.  Torsion dystonia genes in two populations confined to a small region on chromosome 9q32-34. , 1991, American journal of human genetics.

[23]  J. Beckmann,et al.  Study of large inbred Friedreich ataxia families reveals a recombination between D9S15 and the disease locus. , 1992, American journal of human genetics.

[24]  S. Antonarakis,et al.  Methods for cloning large DNA segments as artificial chromosomes in S. cerevisiae. , 1990 .

[25]  D. Schlessinger,et al.  Yeast artificial chromosomes containing human Xq24-Xq28 DNA: library construction and representation of probe sequences. , 1990, Genomics.

[26]  L. S. Cram,et al.  [19] Chromosome sorting by flow cytometry , 1987 .