CTCs detection by SACA chip and image analysis

Immunofluorescence is widely used in detection and characterization of circulating tumor cells (CTCs). The process of immunofluorescence staining on cells needs centrifugal force to wash residual fluorescence, which is not only time consuming but also cells harmful. To speed up the staining process, a three-dimensional (3D) microfluidic device is employed in this work. This study also reports the rapid selection of identified live CTCs from whole blood on SACA chip for further characterization by automatic imaging and in-situ cell capturing process. The whole process takes 120 minutes for examining 4 ml whole blood sample and the recognition sensitivity can approach 1/108 by using the integrated chip and automatic image system. All cells are remained highly viable after the rapid selection process for further applications.

[1]  Kenneth J. Pienta,et al.  Circulating Tumor Cells Predict Survival Benefit From Treatment in Metastatic Castration-Resistant Prostate Cancer Editorial Comment , 2009 .

[2]  K. Isselbacher,et al.  Isolation of circulating tumor cells using a microvortex-generating herringbone-chip , 2010, Proceedings of the National Academy of Sciences.

[3]  Kyung-A Hyun,et al.  Two-stage microfluidic chip for selective isolation of circulating tumor cells (CTCs). , 2015, Biosensors & bioelectronics.

[4]  D. Paulson,et al.  Isolation of a human prostate carcinoma cell line (DU 145) , 1978, International journal of cancer.

[5]  Jason P. Gleghorn,et al.  Capture of circulating tumor cells from whole blood of prostate cancer patients using geometrically enhanced differential immunocapture (GEDI) and a prostate-specific antibody. , 2010, Lab on a chip.

[6]  Yi-Kuen Lee,et al.  Highly efficient capture of circulating tumor cells by using nanostructured silicon substrates with integrated chaotic micromixers. , 2011, Angewandte Chemie.

[7]  D. Parkin,et al.  Global cancer statistics in the year 2000. , 2001, The Lancet. Oncology.

[8]  Inhak Choi,et al.  Circulating tumor cell microseparator based on lateral magnetophoresis and immunomagnetic nanobeads. , 2013, Analytical chemistry.

[9]  Shuang Hou,et al.  Nanostructure Embedded Microchips for Detection, Isolation, and Characterization of Circulating Tumor Cells , 2014, Accounts of chemical research.

[10]  Mehmet Toner,et al.  Inertial Focusing for Tumor Antigen–Dependent and –Independent Sorting of Rare Circulating Tumor Cells , 2013, Science Translational Medicine.

[11]  S Paget,et al.  THE DISTRIBUTION OF SECONDARY GROWTHS IN CANCER OF THE BREAST. , 1889 .

[12]  Michael Morse,et al.  Relationship of circulating tumor cells to tumor response, progression-free survival, and overall survival in patients with metastatic colorectal cancer. , 2008, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[13]  Fan-Gang Tseng,et al.  High-efficiency rare cell identification on a high-density self-assembled cell arrangement chip. , 2014, Biomicrofluidics.

[14]  Bin-Da Chan,et al.  Circulating tumor cell detection using a parallel flow micro-aperture chip system. , 2015, Lab on a chip.

[15]  Guojun Sheng,et al.  EMT in developmental morphogenesis. , 2013, Cancer letters.

[16]  I. Fidler,et al.  The role of the organ microenvironment in the biology and therapy of cancer metastasis , 2007, Journal of cellular biochemistry.

[17]  S. de wit,et al.  Detection of Circulating Tumor Cells , 2014, Scientifica.

[18]  Hellmut Samonigg,et al.  Comparison of two methods for enumerating circulating tumor cells in carcinoma patients , 2005, Cytometry. Part B, Clinical cytometry.

[19]  Maximilian Reichert,et al.  EMT and Dissemination Precede Pancreatic Tumor Formation , 2012, Cell.

[20]  Z. Werb,et al.  Circulating Tumor Cells , 2013, Science.

[21]  Alison Stopeck,et al.  Circulating tumor cells, disease progression, and survival in metastatic breast cancer. , 2004, The New England journal of medicine.

[22]  G. Doyle,et al.  Significance of Circulating Tumor Cells Detected by the CellSearch System in Patients with Metastatic Breast Colorectal and Prostate Cancer , 2009, Journal of oncology.