Three-Domain Heme-c-Cu Nitrite Reductases

Nitrite reductases (NiRs) catalyze the first committed step in denitrification, requiring one electron and two protons for the reduction of nitrite to nitric oxide. NiRs containing two types of copper centers have been studied extensively from several organisms demonstrating that the three substrates (electron, proton, and nitrite) are gated during turnover. Recently structures of a new class of NiRs containing two types of copper centers and a c-type cytochrome have been determined providing new insight into coupling of donor protein (cytochrome) and Cu1-Cu2NiRs. 3D Structure Schematic representation of the trimeric assembly and the monomer structure for RpNiR showing the two types of copper centers and a heme c center (PDB code: 4AX3). All figures were done with PyMOL.1 Keywords: denitrification; nitrite reductase; electron transfer; protein–protein complexes; catalysis

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