Monitoring the Hepatitis A Virus in Oyster from Korea

Hepatitis A virus (HAV) causes many cases of oyster- or clam-associated gastroenteritis in various countries. HAV was detected on oyster by RT-PCR in 19.6% (11/56) in Korea. The percentages of HAV-positive samples in 2011 and 2012 were 27.6% and 11.1%, respectively. Phylogenetic analysis revealed that several nucleotide sequences highly similar to those of HAVs isolated in this study. Phylogenetic analysis of the coding regions of the viral protein VP4/VP2 revealed that all amplicons were classified into IA genogroup. It will provide useful data that aids in our understanding circulating HAVs and may contribute to future control.

[1]  J. Vinjé,et al.  Rapid and Sensitive Detection of Noroviruses by Using TaqMan-Based One-Step Reverse Transcription-PCR Assays and Application to Naturally Contaminated Shellfish Samples , 2005, Applied and Environmental Microbiology.

[2]  A. Zanetti,et al.  Acute hepatitis A in Italy: incidence, risk factors and preventive measures , 2008, Journal of viral hepatitis.

[3]  K. Ishii,et al.  Epidemiological and genetic analyses of a diffuse outbreak of hepatitis A in Japan, 2010. , 2012, Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology.

[4]  H. Cheong.  Vaccination necessary for Korean adults , 2011 .

[5]  D. Lees,et al.  International Standardisation of a Method for Detection of Human Pathogenic Viruses in Molluscan Shellfish , 2010, Food and Environmental Virology.

[6]  Marion Koopmans,et al.  Foodborne viruses: an emerging problem , 2003, International Journal of Food Microbiology.

[7]  G. La Rosa,et al.  Hepatitis A and E Viruses in Wastewaters, in River Waters, and in Bivalve Molluscs in Italy , 2015, Food and Environmental Virology.

[8]  Sung‐Han Kim,et al.  Outbreaks of Gastroenteritis That Occurred during School Excursions in Korea Were Associated with Several Waterborne Strains of Norovirus , 2005, Journal of Clinical Microbiology.

[9]  C. Choi,et al.  The etiology of acute viral hepatitis for the last 3 years at a single institution in Seoul , 2005 .

[10]  Roger I Glass,et al.  Outbreaks of acute gastroenteritis on cruise ships and on land: identification of a predominant circulating strain of norovirus--United States, 2002. , 2004, The Journal of infectious diseases.

[11]  S. Husain,et al.  Hepatitis A viral genotypes and clinical relevance: Clinical and molecular characterization of hepatitis A virus isolates from northern India. , 2005, Hepatology research : the official journal of the Japan Society of Hepatology.

[12]  Moo-Sik Lee,et al.  The Immunogenicity of a Single Dose of Hepatitis A Virus Vaccines (Havrix® and Epaxal®) in Korean Young Adults , 2013, Yonsei medical journal.

[13]  E. Schreier,et al.  Epidemiology of Hepatitis A Virus Infections, Germany, 2007–2008 , 2009, Emerging infectious diseases.

[14]  A. Fiore,et al.  Hepatitis A transmitted by food. , 2004, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[15]  Hee Suk Park,et al.  Risk Factors for Acute Hepatitis A Infection in Korea in 2007 and , 2013 .

[16]  Hee Suk Park,et al.  Risk Factors for Acute Hepatitis A Infection in Korea in 2007 and 2009: A Case-Control Study , 2013, Journal of Korean medical science.

[17]  S. Okitsu,et al.  Detection of norovirus (GI, GII), Sapovirus and astrovirus in fecal samples using reverse transcription single-round multiplex PCR. , 2003, Journal of virological methods.

[18]  N. Neisi,et al.  Molecular epidemiology of hepatitis A virus in patients in the ahwaz region of Iran , 2012, Journal of medical virology.

[19]  R. Gunson,et al.  An outbreak of hepatitis A virus associated with a multi-national inner-city nursery in Glasgow, Scotland. , 2015, Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology.

[20]  A. Vantarakis,et al.  Shellfish-Borne Viral Outbreaks: A Systematic Review , 2012, Food and Environmental Virology.

[21]  M. Pommepuy,et al.  Three-Year Study To Assess Human Enteric Viruses in Shellfish , 2000, Applied and Environmental Microbiology.

[22]  Evaluation of Immunomagnetic Separation Method for the Recovery of Hepatitis A Virus and GI.1 and GII.4 Norovirus Strains Seeded on Oyster and Mussel , 2014, Food and Environmental Virology.

[23]  R. Glass,et al.  Norovirus and Foodborne Disease, United States, 1991–2000 , 2005, Emerging infectious diseases.

[24]  M. Sobsey,et al.  Human Enteric Pathogenic Viruses , 1994 .

[25]  S. M. Kim,et al.  [Clinical features of acute hepatitis A in the Western part of Daejeon and Chungnam province: single center experience]. , 2006, The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi.

[26]  M. Pierson,et al.  Environmental Indicators and Shellfish Safety , 1995 .

[27]  G. Belliot,et al.  Detection and genetic differentiation of human astroviruses: phylogenetic grouping varies by coding region , 1997, Archives of Virology.

[28]  T. Shin,et al.  Detection of hepatitis A virus in seeded oyster digestive tissue by ricin A-linked magnetic separation combined with reverse transcription PCR. , 2015, Journal of Food Protection.

[29]  Mini P. Singh,et al.  Hepatitis A outbreaks. , 2015, The Lancet. Infectious diseases.

[30]  WHO position paper on hepatitis A vaccines – June 2012. , 2012, Releve epidemiologique hebdomadaire.

[31]  Kyung-Yil Lee,et al.  Changing hepatitis A epidemiology and the need for vaccination in Korea. , 2004, Asian Pacific journal of allergy and immunology.

[32]  D. Sano,et al.  Analytical Methods for Virus Detection in Water and Food , 2011 .

[33]  M. Ki,et al.  The changing epidemiology of hepatitis A in children and the consideration of active immunization in Korea. , 2000, Yonsei medical journal.

[34]  A. Bosch,et al.  Human enteric viruses in Coquina clams associated with a large hepatitis A outbreak. , 2001, Water science and technology : a journal of the International Association on Water Pollution Research.

[35]  Y. Khudyakov,et al.  Molecular confirmation of oysters as the vector for hepatitis A in a 2005 multistate outbreak. , 2007, Journal of food protection.

[36]  S. Badur,et al.  Acute hepatitis A virus infection in Turkey , 2008, Journal of medical virology.

[37]  S. Y. Kim,et al.  Genetic analysis of HAV strains isolated from patients with acute hepatitis in Korea, 2005–2006 , 2008, Journal of medical virology.

[38]  S. Paik,et al.  Full-Length Genomic Sequence of Subgenotype IIIA Hepatitis A Virus Isolate in Republic of Korea , 2013, BioMed Research International.

[39]  D. De Medici,et al.  Detection of hepatitis A virus in mussels from different sources marketed in Puglia region (South Italy). , 2002, International journal of food microbiology.

[40]  K. Ishii,et al.  Epidemiological and genetic analyses of a diffuse outbreak of hepatitis A in Japan, 2010. , 2012, Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology.

[41]  D. Lees,et al.  Viruses and bivalve shellfish. , 2000, International journal of food microbiology.

[42]  A. Bosch,et al.  Standardized multiplex one-step qRT-PCR for hepatitis A virus, norovirus GI and GII quantification in bivalve mollusks and water. , 2014, Food microbiology.

[43]  R. Baric,et al.  Detection of norwalk-like virus in shellfish implicated in illness. , 2000, The Journal of infectious diseases.

[44]  S. Conaty,et al.  Hepatitis A in New South Wales, Australia, from consumption of oysters: the first reported outbreak , 2000, Epidemiology and Infection.

[45]  P. Puthavathana,et al.  Surveillance of hepatitis A and E viruses contamination in shellfish in Thailand , 2011, Letters in applied microbiology.

[46]  A. Bosch,et al.  Prevalence of enterovirus and hepatitis A virus in bivalve molluscs from Galicia (NW Spain): inadequacy of the EU standards of microbiological quality. , 2002, International journal of food microbiology.

[47]  D. De Medici,et al.  Detection of hepatitis A virus in shellfish by nested reverse transcription-PCR. , 1999, International journal of food microbiology.

[48]  M. Nei,et al.  MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. , 2007, Molecular biology and evolution.